Repair of articular cartilage defects treated by microfracture and a three-dimensional collagen matrix

Dorotka, Ronald; Windberger, Ursula; Macfelda, Karin; Bindreiter, U.; Toma, Cyril D.; Nehrer, Stefan

doi:10.1016/j.biomaterials.2004.09.034

Cited by 206 publications

(164 citation statements)

References 28 publications

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“…However, a hyaline-like cartilage repair tissue formed after microfracture and covering of the defects with the collagen matrix augmented with chondrocytes. 42,43 Obviously, covering of cartilage defects after microfracture is advantageous for the healing sequence, and may enrich the amount of progenitor cells with chondrogenic differentiation potential at the defect site and may enhance the formation of cartilaginous repair tissue. Consequently, covering of cartilage defects pretreated with microfracture with a cell-free collagen matrix combined with fibrin glue and autologous serum is suggested to be a promising treatment option for cartilage defects.…”

Section: Cell-free Polymer-based Repair In Cartilage Defect Modelmentioning

confidence: 99%

Formation of cartilage repair tissue in articular cartilage defects pretreated with microfracture and covered with cell‐free polymer‐based implants

Erggelet

Endres

Neumann

et al. 2009

Journal Orthopaedic Research

161

118

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The aim of our study was to evaluate the mid-term outcome of a cell-free polymer-based cartilage repair approach in a sheep cartilage defect model in comparison to microfracture treatment. Cell-free, freeze-dried implants (chondrotissue 1 ) made of a poly-glycolic acid (PGA) scaffold and hyaluronan were immersed in autologous serum and used for covering microfractured full-thickness articular cartilage defects of the sheep (n ¼ 4). Defects treated with microfracture only served as controls (n ¼ 4). Six months after implantation, cartilage implants and controls were analyzed by immunohistochemical staining of type II collagen, histological staining of proteoglycans, and histological scoring. Histological analysis showed the formation of a cartilaginous repair tissue rich in proteoglycans. Histological scoring documented significant improvement of repair tissue formation when the defects were covered with the cell-free implant, compared to controls treated with microfracture. Immunohistochemistry showed that the cell-free implant induced cartilaginous repair tissue and type II collagen. Controls treated with microfracture showed marginal formation of a mixed-type repair tissue consisting of cartilaginous tissue and fibro-cartilage. Covering of microfractured defects with the cell-free polymer-based cartilage implant is suggested to be a promising treatment option for cartilage defects and improves the regeneration of articular cartilage. ß

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Section: Cell-free Polymer-based Repair In Cartilage Defect Modelmentioning

confidence: 99%

Formation of cartilage repair tissue in articular cartilage defects pretreated with microfracture and covered with cell‐free polymer‐based implants

Erggelet

Endres

Neumann

et al. 2009

Journal Orthopaedic Research

161

118

View full text Add to dashboard Cite

show abstract

“…Recently a huge expansion in biomaterial technologies, scaffolds, cell sources, and molecular and genetic manipulations took place to create functional tissue replacements to treat cartilage injuries or osteoarthritis [38][39][40] . A new generation of materials is being developed and it is influenced by the knowledge of the anatomical and structural complexity of articular cartilage.…”

Section: Biomaterialsmentioning

confidence: 99%

New perspectives for articular cartilage repair treatment through tissue engineering: A contemporary review

Musumeci

Castrogiovanni

Leonardi

et al. 2014

WJO

133

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“…For each sample five randomly chosen areas were assessed in 40x magnification. Cell morphology was graded as spherical (length:width ratio smaller 2:1), elongated (length:width ratio 2:1), or unassigned, as previously described [16][17][18]. The spherical shape is associated with chondrocytic differentiation; the elongated shape is associated with fibroblastic differentiation.…”

Section: Histological and Immunohistochemical Analysismentioning

confidence: 99%

Polyethylene terephthalate (PET) enhances chondrogenic differentiation of ovine meniscocytes in a hyaluronic acid/polycaprolactone scaffold in vitro

Koller

Nehrer

Vavken

et al. 2012

International Orthopaedics (SICOT)

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Purpose The purpose of this study was to assess the effect of polyethylene terephthalate (PET) on proliferation, differentiation, and attachment of ovine meniscocytes seeded in a hyaluronic acid/polycaprolactone biomaterial (BF-1) Methods BF-1 (30 % hyaluronic acid and 70 % polycaprolactone) cylinders with PET (CO-PET) or without PET, were seeded with 2x10 6 ovine meniscus cells. The specimens were harvested in triplets at 12 hours, seven, 14, 21 and 28 days. DNA content was measured to test proliferation, histological analysis for cell morphology, and biochemical assessment of glycosaminoglycan content and RT-PCR for type I and II collagen were used to assess differentiation, with immunohistochemistry as post-translational control. Attachment was evaluated by electronic microscopy at 28 days. Results DNA content was consistent and equal across groups, suggesting no effect of PET on cell proliferation. However, the BF-1 CO-PET showed a higher percentage of cells with spherical morphology which is typical for a chondrocytic phenotype. This biomaterial with PET also showed a higher type II collagen mRNA expression and an eightfold higher GAG-content than the material without PET. Small amounts of type I collagen mRNA expression were present on both materials at all time points. PCR results were confirmed by immunohistochemistry. Conclusion Addition of PET to a hyaluronic acid/polycaprolactone biomaterial enhances a cartilaginous phenotype, increased type II collagen mRNA expression and a higher GAG production in ovine mensicocytes.

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Repair of articular cartilage defects treated by microfracture and a three-dimensional collagen matrix

Cited by 206 publications

References 28 publications

Formation of cartilage repair tissue in articular cartilage defects pretreated with microfracture and covered with cell‐free polymer‐based implants

Formation of cartilage repair tissue in articular cartilage defects pretreated with microfracture and covered with cell‐free polymer‐based implants

New perspectives for articular cartilage repair treatment through tissue engineering: A contemporary review

Polyethylene terephthalate (PET) enhances chondrogenic differentiation of ovine meniscocytes in a hyaluronic acid/polycaprolactone scaffold in vitro

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