Repair of full-thickness articular cartilage defects using injectable type II collagen gel embedded with cultured chondrocytes in a rabbit model

Funayama, Atsushi; Niki, Yasuo; Matsumoto, Hideo; Maeno, Shinichi; Yatabe, Taku; Morioka, Hideo; Yanagimoto, Shigeru; Taguchi, Tetsushi; Tanaka, Junzo; Toyama, Yoshiaki

doi:10.1007/s00776-008-1220-z

Cited by 91 publications

(60 citation statements)

References 20 publications

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“…The mean overall outcome evaluated with the modified O'Driscoll score was about 20 points (17), as in the present study.…”

Section: Discussionsupporting

confidence: 81%

“…In fact, injections of type II collagen induced arthritis in rats (12), primates (13) and mice (14), and antibodies to type II collagen were shown playa major role in the initiation of the arthritic process (15) and their presence was detected in rheumatoid arthritis patients (16). Although type II collagen scaffolds have been tested in animal models without apparent adverse responses, none of these studies have been specifically designed to look for immunological reactions (8,10,11,17) and in particular the proliferation of synovial cells and the infiltration of leukocytes in the synovial membrane that are fundamental events in the development of joint inflammation (18).…”

mentioning

confidence: 99%

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Mixed Type I and Type II Collagen Scaffold for Cartilage Repair: Ultrastructural Study of Synovial Membrane Response and Healing Potential versus Microfractures (A Pilot Study)

Enea

Guerra

Roggiani

et al. 2013

Int J Immunopathol Pharmacol

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The first two authors contributed equally to the manuscriptThe association between microfracture ofthe subchondral plate and a coverage scaffold has emerged as a promising strategy to treat cartilage lesions in a one-step procedure. Between different types of scaffolds (e.g, collagen, hyaluronic acid, polyglycolic acid) currently studied, type I collagen scaffold is the most used for this purpose, and is currently adopted for humans. The aim of this study was to test a novel scaffold made of mixed type I and II collagen (I-IICS) in order to define the immunological reaction of the synovial tissue and the repair capabilities induced by the collagen membrane when associated with microfracture. Eight New Zealand White rabbits, aged 180 days, were operated on bilaterally on the medial femoral condyle. A circular cartilage lesion was performed up to the calcified layer of the medial femoral condyle, and the centre of the lesion was microfractured. Randomly, one of the two lesions was covered with the I-IICS ( treated), and the other was left uncovered (control). The synovial membrane reaction and the quality of the cartilage tissue repair were investigated at 2, 90,180 and 270 days macroscopically, histomorphologically and ultrastructurally. Expression oftumor necrosis factor-alpha (TNF-a) in synovial tissue by immunocytochemistry analyses was also investigated. In the control group, at 2 days gold particles were localized mainly on synoviocyte type A, less on synoviocytes type B and on collagen bundles; in the treated group the reaction is more intense in cells in the matrix, but at 180 days controls and treated joints were very similar. The synovial membranes of the joints receiving the I-IICS did not reveal significant changes compared to the age-matched controls. Signs of inflammation were present at the 90-day time-point, and became less evident at afterwards. The degradation of the scaffolds was already evident at the 90-day time-point. The quality of the cartilage repair of the rabbits treated with the I-IICS was slightly better in 5 cases out of 6 in comparison to the controls. However, a statistically significant difference was not detected (p=0.06). Scaffolds made of mixed type I and II collagen exhibited good biocompatibility properties in vivo and favored cartilage restoration when associated with microfracture, as shown in this pilot study.

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“…The mean overall outcome evaluated with the modified O'Driscoll score was about 20 points (17), as in the present study.…”

Section: Discussionsupporting

confidence: 81%

mentioning

confidence: 99%

Mixed Type I and Type II Collagen Scaffold for Cartilage Repair: Ultrastructural Study of Synovial Membrane Response and Healing Potential versus Microfractures (A Pilot Study)

Enea

Guerra

Roggiani

et al. 2013

Int J Immunopathol Pharmacol

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“…Collagen sponge expands to form a colloid-like absorbent material with excellent plasticity, lubricity and stability. It had been reported that collagen, collagen gel and collagen sponge could be used in repairing cartilage defects, while a combination of collagen gel and cartilage cells effectively could repaired the cartilage defects effectively [13][14][15][16]. Nevertheless, neither collagen gel alone nor combined with cartilage cells achieved the desired clinical effect due to such problems as the incapabcity of proliferation of mature cartilage cells in vivo.…”

Section: Construction Of Tissue-engineered Cartilage Composed Of Hpdsmentioning

confidence: 91%

Construction of tissue-engineered cartilage using human placenta-derived stem cells

Liu

Hui

Chai

et al. 2010

Sci. China Life Sci.

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Human placenta-derived stem cells (hPDSCs) were isolated by trypsinization and further induced into cartilage cells in vitro. The engineered cartilage was constructed by combining hPDSCs with collagen sponge and the cartilage formation was observed by implantation into nude mice. Results showed that hPDSCs featured mesenchymal stem cells and maintained proliferation in vitro for over 30 passages while remaining undifferentiated. All results indicated that hPDSCs have the potential to differentiate into functional cartilage cells in vitro when combined with collagen sponge, which provided experimental evidence for prospective clinical application.

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“…Para a avaliação macroscópica, foi utilizado o teste de Goodman para contrastes entre e dentro de populações binomiais (Goodman, 1964;1965). Todas as comparações foram realizadas considerando-se 5% de significância (Zar, 1999).…”

Section: Methodsunclassified

Reparação de defeitos osteocondrais de cães com implante de cultura de condrócitos homólogos e membrana biossintética de celulose: avaliação clínica, ultrassonográfica e macroscópica

Iamaguti¹,

Brandão²,

Mota³

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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Avaliou-se o implante de condrócitos homólogos em lesões osteocondrais, utilizando a membrana biossintética à base de celulose (MBC) como revestimento. Dez cães adultos e clinicamente sadios foram submetidos à artrotomia das articulações fêmoro-tíbio-patelares. Defeitos de quatro milímetros de diâmetro por quatro milímetros de profundidade foram induzidos na tróclea femoral de ambos os membros. A MBC foi aplicada na base e superfície das lesões. Os defeitos do membro direito foram preenchidos com condrócitos homólogos cultivados e formaram o grupo tratado (GT); e os defeitos do membro esquerdo, sem implante celular, formaram o grupo controle (GC). Os animais foram avaliados clínica e ultrassonograficamente aos 30 e 60 dias. A evolução pós-operatória dos cães foi analisada com especial interesse nos processos de reparação da lesão, por meio de macroscopia. Não houve diferença clínica e ultrassonográfica entre os grupos. Entretanto, à macroscopia, ocorreu maior prevalência de formação de tecido cicatricial esbranquiçado no GT. O tecido neoformado apresentou melhor qualidade associado ao implante homólogo de condrócitos, mas não promoveu reparação por cartilagem hialina.

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Repair of full-thickness articular cartilage defects using injectable type II collagen gel embedded with cultured chondrocytes in a rabbit model

Cited by 91 publications

References 20 publications

Mixed Type I and Type II Collagen Scaffold for Cartilage Repair: Ultrastructural Study of Synovial Membrane Response and Healing Potential versus Microfractures (A Pilot Study)

Mixed Type I and Type II Collagen Scaffold for Cartilage Repair: Ultrastructural Study of Synovial Membrane Response and Healing Potential versus Microfractures (A Pilot Study)

Construction of tissue-engineered cartilage using human placenta-derived stem cells

Reparação de defeitos osteocondrais de cães com implante de cultura de condrócitos homólogos e membrana biossintética de celulose: avaliação clínica, ultrassonográfica e macroscópica

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