Repair of injured spinal cord using platelet-rich plasma- and endometrial stem cells-loaded chitosan scaffolds

Ai, Jafar; Farzin, Ali; Zamiri, Sina; Hadjighassem, Mahmoudreza; Ebrahimi‐Barough, Somayeh; Ai, Armin; Mohandesnezhad, Sanam; Karampour, Ahmad; Farahani, Morteza Sagharjoghi; Goodarzi, Arash

doi:10.1080/00914037.2020.1772257

Cited by 7 publications

(6 citation statements)

References 50 publications

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“…Also, HUCB-PRP dramatically increased MAG expression and decreased GSK3β, caspase3 and CSF-tau levels compared to the injury group. Previous studies showed that PRP promoted or/and help axonal regeneration and sprouting of axons in the SCI models (14,(26)(27)(28)(29)(30)69). Similar to the results from Salarinia's study (29) we showed that PRP injection reduced apoptosis which resulted in an increased in the MAG level and reduction in the destruction of oligodendrocytes.…”

Section: Discussionsupporting

confidence: 88%

“…It has previously mentioned that PRP delivered from umbilical cord has richer growth factors than adult PRP (31,40). A few studies have examined the effect of PRP on axonal regeneration and remyelination of the spinal cord injury models (14,(26)(27)(28)(29)(30).…”

Section: Discussionmentioning

confidence: 99%

“…Also in 2020, a study stated that in chitosan scaffolds lled with PRP and human endometrial stem cells (hEnSCs), PRP increased the survival of hEnSCs cells and prevented the formation of scare tissue. It also was responsible for angiogenesis and regeneration of the nerve bers (28).…”

Section: Discussionmentioning

confidence: 99%

“…It has also been indicated to have bene cial effects on the growth, neuroprotection, anti-apoptosis and regeneration of peripheral nerves such as the sciatic nerve (20)(21)(22), facial nerve (23) and the cavernous nerve (24,25). The studies have examined the effect of PRP in peripheral blood on axonal regeneration and remyelination of the SCI models (14,(26)(27)(28)(29)(30). Studies indicated that the existence of numerous growth factors such as FGF, EGF, VEGF, IGF-1, interleukins and interferons in PRP is responsible for inducing many of its bene cial effects (31).…”

Section: Introductionmentioning

confidence: 99%

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Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Behroozi

Ramezani

Nasirinezhad

2021

Preprint

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Background: There are complex mechanisms for reducing intrinsic repair ability and neuronal regeneration following spinal cord injury (SCI). Platelet-rich plasma (PRP) is a rich source of growth factors and has been used to stimulate regeneration of peripheral nerves in degenerationtive diseases. However, only a few studies have investigated the effects of PRP on the SCI models. We examined whether PRP derived from human umbilical cord blood (HUCB-PRP) could recover motor function in animals with spinal cord injury. We also investigate the role of Wnt signaling pathway.Methods: Ault male Wistar rats were randomly divided into 6 groups (n=60) as control, sham, SCI, vehicle (SCI+platelet-poor plasma), PRP2day (SCI+injection 2 days after SCI) and PRP14day (SCI+injection 14 days after SCI). SCI was performed at the T12-T13 level. BBB tests were done weekly after injury for six weeks. caspase3 expression was determined using the Immunohistochemistry technique. The expression of GSK3β, Tau and MAG were determined using the Western blot technique. Data were analyzed by PRISM & SPSS software. Results: PRP injected animals showed a higher locomotor function recovery than those in the SCI group (p<0.0001). The level of caspase3, GSK3β and CSF- Tau reduced and MAG level in the spinal cord increased by injection of HUCB-PRP in animals with spinal cord injury. Conclusions: Injection of HUCB-PRP enhanced hind limb locomotor performance by modulation of caspase3, GSK3β, tau and MAG expression. Using HUCB-PRP could be a new therapeutic option for recovering the motor function and axonal regeneration after spinal cord injury.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Behroozi

Ramezani

Nasirinezhad

2021

Preprint

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“…In some cases, they are used as drug-releasing agents to enhance the regenerative processes [ 92 ]. Structurally, the scaffolds must be three-dimensional, with a good porosity for exchanging nutrients and cellular waste material with the surrounding medium [ 93 ].…”

Section: Scaffolds For Cardiac Tissue Regenerationmentioning

confidence: 99%

Chitosan-Based Scaffolds for the Treatment of Myocardial Infarction: A Systematic Review

2023

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Cardiovascular diseases (CVD), such as myocardial infarction (MI), constitute one of the world’s leading causes of annual deaths. This cardiomyopathy generates a tissue scar with poor anatomical properties and cell necrosis that can lead to heart failure. Necrotic tissue repair is required through pharmaceutical or surgical treatments to avoid such loss, which has associated adverse collateral effects. However, to recover the infarcted myocardial tissue, biopolymer-based scaffolds are used as safer alternative treatments with fewer side effects due to their biocompatibility, chemical adaptability and biodegradability. For this reason, a systematic review of the literature from the last five years on the production and application of chitosan scaffolds for the reconstructive engineering of myocardial tissue was carried out. Seventy-five records were included for review using the “preferred reporting items for systematic reviews and meta-analyses” data collection strategy. It was observed that the chitosan scaffolds have a remarkable capacity for restoring the essential functions of the heart through the mimicry of its physiological environment and with a controlled porosity that allows for the exchange of nutrients, the improvement of the electrical conductivity and the stimulation of cell differentiation of the stem cells. In addition, the chitosan scaffolds can significantly improve angiogenesis in the infarcted tissue by stimulating the production of the glycoprotein receptors of the vascular endothelial growth factor (VEGF) family. Therefore, the possible mechanisms of action of the chitosan scaffolds on cardiomyocytes and stem cells were analyzed. For all the advantages observed, it is considered that the treatment of MI with the chitosan scaffolds is promising, showing multiple advantages within the regenerative therapies of CVD.

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Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells

Kim

Choi

et al. 2020

Macromol. Res.

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Repair of injured spinal cord using platelet-rich plasma- and endometrial stem cells-loaded chitosan scaffolds

Cited by 7 publications

References 50 publications

Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Chitosan-Based Scaffolds for the Treatment of Myocardial Infarction: A Systematic Review

Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells

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