Repair of tendons treated with peracetic acid–ethanol and gamma irradiation by EDC combined with NHS: a morphological, biochemical and biomechanical study in vitro

Ma, Rong-Xing; Li, Ruifeng; Xiaoqiang, Deng; Qiao, Rui‐qi; Li, Ji-kai; Song, Kun-xiu; Ji, Shao-lin; Hu, Yongcheng

doi:10.1007/s10561-023-10080-6

Cited by 1 publication

(1 citation statement)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Speci cally, as α-chymotrypsin only degrades collagen bers with compromised triple helix structures, enzymolysis resistance testing effectively re ects the stability of collagen and the degree of consequent damage. As damaged tendons tend to release a higher content of free hydroxyproline when digested by α-chymotrypsin [55], this important structural support factor for trihelix proteins, particularly when located at the X-position, appears to play a crucial role in stabilizing the triple helix structure. Moreover, the Y-position enhances the thermal stability of this structure [56].…”

Section: Biomechanical Testmentioning

confidence: 99%

Temperature-based protection of biochemical and biomechanical characteristics during 25 kGy 60Co irradiation for allograft tendons

Wan,

Liu,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Nowadays, there is a lack of effective treatment for reducing damage to the collagen proteins in tendons during the gamma irradiation process. We aim to investigate different temperatures on the protection of allogeneic tendons during 25kGy 60Co gamma irradiation. Methods: Based on different temperatures during irradiation, twenty-four allogeneic tendons were divided into groups of -70 °C, 0 °C, and room temperature (RT). The arrangement and spatial structure of collagen fibers were observed by HE and Masson staining, polarizing microscope after Sirius Red staining was used to observe collagen curl periods, damaged collagen protein was assessed using free hydroxyproline tests. Tensile fracture and cyclic loading tests were performed, then calculated the elastic modulus, maximum stress, maximum strain, strain energy density, and cyclic creep strain. Results: In the -70 °C group, the structure and morphology of collagen fibers were significantly better than in the 0 °C group. At -70 ºC the damaged collagen was the lowest, with 46.27 and 50.03% improvement compared to that at 0ºC (P = 0.016) and RT (P < 0.004). The maximum stress of -70 °C (68.19 ± 30.11 MPa) is superior to that at 0 °C (36.58 ± 12.00 MPa) and RT (41.27 ± 16.34 MPa). Conclusions: Cryogenic temperature has a protective effect on allograft tendons at 25kGy gamma radiation, and -70 °C provides better protection than 0 °C

show abstract

Section: Biomechanical Testmentioning

confidence: 99%