Repairing whole facial nerve defects with xenogeneic acellular nerve grafts in rhesus monkeys

Zhu, Guochen; Xiao, Dajiang; Zhu, Bi-Wen; Xiao, Yan

doi:10.4103/1673-5374.324853

Cited by 7 publications

(3 citation statements)

References 54 publications

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“…ANAs were prepared by chemical extraction of the Sondell's scheme and our slight modi cation from the tibial nerves of 5 adult rats [24,25]. In the ANA + cells group, 10 µL of CelTrac1000-labeled EPI-NCSCs with concentration of 5 × 10 7 cells/mL was micro-injected into the 10-mm-long ANAs from one end.…”

Section: Construction Of Nerve Transplantationmentioning

confidence: 99%

“…In the present study, CelTrac1000-labeled EPI-NCSCs were micro-injected into the acellular nerve allografts (ANAs) to bridge the 10-mm defect of the rat facial nerve [24,25]. Then, we traced CelTrac1000labeled EPI-NCSCs by NIR-II optical imaging system, and obtained a temporospatial map of the behavior of EPI-NCSCs in vivo for up to 14 weeks.…”

Section: Introductionmentioning

confidence: 99%

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Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Lv,

Zhu,

Zhang

et al. 2023

Preprint

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Background Tissue engineering based on stem cells has achieved satisfactory results in repairing facial nerve defects. However, the in vivo process of the transplanted cells has not been fully clear until now, although it is critical to understand the process and the underlying mechanism of regeneration for better therapeutic outcomes. Recently, second near-infrared window (NIR-II) fluorescence imaging has emerged as a rapidly evolving bio-imaging technique capable of visualizing and quantifying biological processes at the cellular level of living organisms. Methods Firstly, rat hair follicle epidermal neural crest stem cells (EPI-NCSCs) were isolated, cultured and identified by expression of SOX10 and Nestin, and then labeled with CelTrac1000. Rat acellular nerve allografts (ANAs) were prepared by chemical extraction. Secondly, 30 adult male rats were randomly and equally assigned into three groups: ANA + cells group, ANA group, and autograft group. The buccal branch of the facial nerve on right side was exposed and a 10-mm-long gap was bridged by ANA laden with CelTrac1000-labeled EPI-NCSCs, ANA laden with CelTrac1000 dye, and autologous nerve, respectively. Thirdly, CelTrac1000-labeled EPI-NCSCs were detected by NIR-II optical imaging system to visualize the behavior of the transplanted cells in vivo postoperatively. Finally, vibrissa movement, compound muscle action potentials (CMAPs) of vibrissal muscle, facial motoneurons retrotraced by Fluorogold, morphology and histology of the regenerated nerves in three groups were analyzed after surgery, respectively. Results Through 14 weeks of dynamic observation, we found that EPI-NCSCs successfully survived in the ANAs in vivo. Meanwhile, the region of the NIR-II fluorescence signals was gradually limited to be consistent with the route of the regenerative segment of the facial nerve. Furthermore, the degree of the vibrissa movement, the recovery value of the onset latency and amplitude of CMAPs, the number of Fluorogold-labeled cells, the CD31 positive area/total area, the mean gray value of S100 and β-tubulin III, the number and the diameter of the myelinated nerve fibers in the ANA group were lower than the other two groups (P < 0.05), and the other two groups had similar values (P > 0.05). Additionally, the thickness of the myelin sheaths was the thinnest in the ANA group, and the thickest in the autograft group (P< 0.05). Conclusions The migration map of local CelTrac1000-labeled EPI-NCSCs was successfully monitored by the NIR-II fluorescence imaging system when EPI-NCSCs within the ANAs were applied to treat rat facial nerve defects. Additionally, EPI-NCSCs promoted the ANAs to repair facial nerve defects in a small animal model.

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Section: Construction Of Nerve Transplantationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Lv,

Zhu,

Zhang

et al. 2023

Preprint

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“…In a monkey model, 8 months after facial nerve defect reconstruction, Zhu proved the advantage of using xenografts obtained from guinea pigs compared to autotransplants. The aforementioned experimental studies indicate the possibility of using xenografts in future work [19].…”

Section: B) Xenograftsmentioning

confidence: 99%

Application of Different Techniques in Facial Nerve Reconstruction on Experimental Models

et al. 2023

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The facial nerve is the main motor nerve that activates the mimic musculature of the face. Nerve tree injuries lead to paralysis of the same half of the face. In addition to functional damage in the form of difficulty in chewing, speaking and closing the eyes, paresis is the cause of pronounced emotional and social problems in patients. The etiology of facial nerve damage is diverse, including trauma, neurological, infectious, metabolic, vascular, neoplastic processes, as well as idiopathic and iatrogenic. Of special importance to us are iatrogenic injuries of the transcranial part of the facial nerve, which most often occur as part of surgical interventions in the area of the parotid lodge, where the nerve itself branches into its final branches that innervate facial muscles. A high degree of iatrogenic damage over 40% requires the timely application of adequate techniques and materials for the purpose of neuroregeneration. Considering the delicacy of the function of the facial nerve and the consequences of its damage, previous methods described in some studies, used in the regeneration process are based on the summarized results of experimental studies on different animal models. A prerequisite for the success of an experimental study is certainly the anatomical compatibility of experimental animals and humans. Based on the analyzed research, in the last decade rats were used the most, which can be linked to acceptable maintenance costs. Nevertheless, rabbits represent an ideal experimental model due to their anatomical and functional similarities with humans, in which it is possible to successfully simulate paralysis of the facial nerve and monitor the recovery of mimic musculature using the method of movement visualization and electromyography.

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Predicting in vivo therapeutic efficacy of CelTrac1000-labeled hair follicle epidermal neural crest stem cells in models of repairing rat facial nerve defects via second near-infrared fluorescence imaging

Lv,

Zhu,

et al. 2024

Life Sciences

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Repairing whole facial nerve defects with xenogeneic acellular nerve grafts in rhesus monkeys

Cited by 7 publications

References 54 publications

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Application of Different Techniques in Facial Nerve Reconstruction on Experimental Models

Predicting in vivo therapeutic efficacy of CelTrac1000-labeled hair follicle epidermal neural crest stem cells in models of repairing rat facial nerve defects via second near-infrared fluorescence imaging

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