Repeated hormonal treatment and laparoscopic ovum pick-up followed by in vitro embryo production in goats raised in the tropics

Sanchez, Deisy Johana Diaz; Melo, Cynthia de Freitas; Souza-Fabjan, J. M. G.; Sousa, Francisco Carlos de; Rocha, Amanda Albuquerque; Campelo, Iana S.; Teixeira, Dárcio Ítalo Alves; Pereira, Alexsandra Fernandes; Melo, Luciana Magalhães; Freitas, V. J. F.

doi:10.1016/j.livsci.2014.04.012

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…The incidence of injuries caused to the ovary after successive LOPU sessions has been under study. In general, most of the reports did not found severe ovarian lesions that interfere with follicular aspiration, oocyte retrieval or embryo yield after 3 to 7 repeated hormonal treatments and LOPU sessions (Pierson et al, 2004;Gibbons et al, 2007;Teixeira et al, 2011;Sanchez et al, 2014). Other authors reported the presence of small adhesions in around 30% of sheep after repeated LOPU over a 10-week period (McEvoy et al, 2006).…”

Section: Follicular Aspiration By Laparoscopymentioning

confidence: 99%

Advances and limitations of in vitro embryo production in sheep and goats

Menchaca¹,

Barrera²,

Santos³

et al. 2016

Anim Reprod

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This review summarizes the latest advances and main limitations for the implementation of in vitro embryo production programs in sheep and goats. We describe the laparoscopic assisted technique for oocyte retrieval and propose new insights for follicular manipulation to improve oocyte quality. Further description of the routine conducted in our laboratory for the in vitro process of oocyte maturation, fertilization and embryo culture is presented, with emphasis in the main issues for the success of the technique. Protocols for fixed time embryo transfer (FTET) are proposed and the optimal number of in vitro produced (IVP) embryos to be transferred per female is discussed. In addition, we present pregnancy outcomes and birth rates recently obtained with FTET with IVP embryos cryopreserved by vitrification with new minimum volume methods. In summary, due to important refinements for in vitro embryo production in sheep and goats achieved in the recent years, this technology is now available for its implementation in commercial programs for genetic improvement, for the production of genetically engineered sheep and goats, and for basic research in reproduction.

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Section: Follicular Aspiration By Laparoscopymentioning

confidence: 99%

Advances and limitations of in vitro embryo production in sheep and goats

Menchaca¹,

Barrera²,

Santos³

et al. 2016

Anim Reprod

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“…Many authors have reported an association between LOPU repetition and increased variability in the individual response of donors [9,35]. On the other hand, Sanchez et al [10] did not find any decrease in the oocyte recovery rate in repeatedly stimulated goats with collection at every 2 weeks. In our experiment, five animals showed omental and ovarian adhesions in the third LOPU session.…”

Section: Discussionmentioning

confidence: 99%

“…However, some recent studies have shown contrary results, showing no negative effect of repeated ovarian stimulation followed by LOPU on the quantity and quality of oocytes recovered with porcine FSH (pFSH) [4]. Sanchez et al [10] reported that goats raised in the tropics, hormonally stimulated with pFSH, and then subjected to LOPU, using 7 repetitions every 2–3 weeks, maintained good ovarian response and embryo production. Additionally, Gibbons et al [5], aiming to maximize IVP in goats and sheep, observed that stimulation using the one-shot regimen (FSH plus eCG) on every four day followed by LOPU, totaling three sessions, maintained the same pattern of ovarian response and embryo production.…”

Section: Introductionmentioning

confidence: 99%

Effect of Continuous Administration of Enalapril Maleate on the Oocyte Quality and In Vitro Production of Parthenote Embryos in Nulliparous and Multiparous Goats Undergoing Serial Laparoscopic Ovum Pick-Up

Bravo

Moreno

Fernandes

et al. 2019

Animals

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Simple SummaryOne of the main obstacles to the in vitro production of embryos in goats is the low ovarian response to hormonal treatments and low oocyte quality. Thus, several strategies have been performed to improve the reproductive performance of goats, including the development of new hormonal protocols as well as the use of other drugs that act directly or indirectly on reproductive function. In this experiment, we tested the use of a hormonal protocol aimed at maximizing the ovarian response and, in parallel, an angiotensin-converting enzyme (ACE) inhibitor was administered daily as an adjuvant. In recent years, the renin-angiotensin system has been shown to play an important role in reproductive function, especially in follicular development. We found that administration of the ACE inhibitor affected the ovarian response in multiparous goats, with more visible follicles, and had no effect on oocyte quality or during embryonic development, thus being a possible alternative to improve goat reproductive response.AbstractThe aim of this work was to determine the effect of enalapril maleate administration, during oocyte recovery by serial laparoscopic ovum pick-up (LOPU), on the ovarian response and in vitro embryo production (IVP). Twenty cross-bred goats were allocated equally into two groups: Nulliparous and Multiparous. In each group, five animals were selected to receive daily doses of enalapril maleate during the hormonal protocol. Estrus was synchronized by a PGF2α analog, followed 48 h later by insertion of an intravaginal device with progesterone. Forty-eight hours after, a single dose of FSH/eCG was administered. The FSH/eCG doses were repeated three times, on every four day. Oocytes were recovered by LOPU 24 h after each FSH/eCG dose. Viable oocytes were matured in vitro, to be parthenogenetically activated and cultured for 72 h to the cleavage stage. The drug treatment increased the proportion of total follicles observed at LOPU (p < 0.01) in multiparous goats. In both parity groups, enalapril administration had no effect on the proportion or quality of oocytes recovered. Furthermore, the number of embryos cleaved was similar between the groups. Thus, enalapril maleate affected the ovarian response in multiparous animals only and had no effect on the oocyte quality or IVP.

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“…Table 3. Punctured follicles, recovered cumulus-oocyte complexes (COCs), and recovery rate in goats hormonally treated for seven times per session [25]. The values are mean (±SD) and percentages.…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

The Use of Reproductive Technologies to Produce Transgenic Goats

Freitas¹,

Melo²,

Teixeira³

et al. 2016

Insights From Animal Reproduction

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Recombinant DN" technology has revolutionized the production of therapeutic proteins. Thus, genes of a great number of human proteins have already been identified and cloned. The use of farm animals as bioreactors may be the better choice to produce recombinant therapeutic proteins. For this activity, the term pharming was created, referring to the use of genetic engineering to obtain a transgenic or genetically modified animal. Considering the advantages and disadvantages of livestock species, goats appear as a very good model. In addition, the first human commercially approved biological drug antithrombin "T was produced from the milk of transgenic goats. The aim of this chapter is to present various reproductive technologies used to obtain transgenic goats secreting recombinant proteins in milk. Initially, this chapter presents the methods for embryo production in vivo and in vitro to realize the DN" microinjection in pronuclear embryos. Thus, the techniques of superovulation of donors in vivo embryo production and ovarian stimulation for oocyte recovery in vitro embryo production are described. "lso, the methods for DN" microinjection and embryo transfer are detailed in this chapter. Finally, this chapter describes the reproductive procedures used for obtaining transgenic goats by cloning.

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Repeated hormonal treatment and laparoscopic ovum pick-up followed by in vitro embryo production in goats raised in the tropics

Cited by 7 publications

References 13 publications

Advances and limitations of in vitro embryo production in sheep and goats

Advances and limitations of in vitro embryo production in sheep and goats

Effect of Continuous Administration of Enalapril Maleate on the Oocyte Quality and In Vitro Production of Parthenote Embryos in Nulliparous and Multiparous Goats Undergoing Serial Laparoscopic Ovum Pick-Up

The Use of Reproductive Technologies to Produce Transgenic Goats

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