Repeated maternal separation of male Wistar rats alters glutamate receptor expression in the hippocampus but not the prefrontal cortex

Pickering, Chris; Gustafsson, Lisa; Cebere, Aleta; Nylander, Ingrid; Liljequist, Sture

doi:10.1016/j.brainres.2006.04.136

Cited by 107 publications

(74 citation statements)

References 39 publications

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“…Other consequences of maternal separation in the adult brain include elevated ACTH levels in response to a stressor (35), altered CRF binding sites in several brain areas (92,133,172), dysregulated glucocorticoid and mineralocorticoid receptor expression (92,137,179), and alterations in the serotonergic (35,162,173) and glutamatergic systems (129). In a model of repeated maternal deprivation, it has been shown that alterations of brain lipid peroxidation and the antioxidant enzymes activities are age-and sex-dependent (170).…”

Section: Schiavone Et Almentioning

confidence: 99%

Severe Life Stress and Oxidative Stress in the Brain: From Animal Models to Human Pathology

Schiavone

Jaquet

Trabace

et al. 2013

Antioxidants & Redox Signaling

289

211

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Significance: Severe life stress (SLS), as opposed to trivial everyday stress, is defined as a serious psychosocial event with the potential of causing an impacting psychological traumatism. Recent Advances: Numerous studies have attempted to understand how the central nervous system (CNS) responds to SLS. This response includes a variety of morphological and neurochemical modifications; among them, oxidative stress is almost invariably observed. Oxidative stress is defined as disequilibrium between oxidant generation and the antioxidant response. Critical Issues: In this review, we discuss how SLS leads to oxidative stress in the CNS, and how the latter impacts pathophysiological outcomes. We also critically discuss experimental methods that measure oxidative stress in the CNS. The review covers animal models and human observations. Animal models of SLS include sleep deprivation, maternal separation, and social isolation in rodents, and the establishment of hierarchy in non-human primates. In humans, SLS, which is caused by traumatic events such as child abuse, war, and divorce, is also accompanied by oxidative stress in the CNS. Future Directions: The outcome of SLS in humans ranges from resilience, over post-traumatic stress disorder, to development of chronic mental disorders. Defining the sources of oxidative stress in SLS might in the long run provide new therapeutic avenues.

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Section: Schiavone Et Almentioning

confidence: 99%

Severe Life Stress and Oxidative Stress in the Brain: From Animal Models to Human Pathology

Schiavone

Jaquet

Trabace

et al. 2013

Antioxidants & Redox Signaling

289

211

View full text Add to dashboard Cite

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“…The Hes-1 primers were purchased from QIAGEN (catalog #QTO1081094) for the purpose of SYBR Green real-time PCR. The primers for GluR1 were designed according to that of Pickering et al (2006). The sequence for the forward primer was as follows: 5Ј-GCTTCATGGACATTGACTTA-3Ј, and that for the reverse primer was as follows: 5Ј-ATCTCAAGTCGGTAGGAG TA-3Ј.…”

Section: Methodsmentioning

confidence: 99%

JNK1 Inhibits GluR1 Expression and GluR1-Mediated Calcium Influx through Phosphorylation and Stabilization of Hes-1

Lin

Lee²

2012

J. Neurosci.

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The GluR1 subunit of the AMPA receptor plays an important role in excitatory synaptic transmission and synaptic plasticity in the brain, but the regulation mechanism for GluR1 expression is largely unknown. Hairy and enhancer of split 1 (Hes-1) is a mammalian transcription repressor that regulates neuronal differentiation and development, but the role of Hes-1 in differentiated neurons is also less known. Here, we examined the molecular mechanism in regulation of GluR1 expression in rat cultured cortical neurons. We found that Hes-1 suppressed GluR1 promoter activity and decreased GluR1 expression through direct binding to the N-box and through preventing Mash1/E47 from binding to the E-box of GluR1 promoter. We also found that Hes-1 could be regulated by c-Jun N-terminal kinase (JNK1). JNK1 directly phosphorylates Hes-1 at Ser-263. Furthermore, JNK1 phosphorylation of Hes-1 stabilized the Hes-1 protein and enhanced the suppressing effect of Hes-1 on GluR1 expression. These effects were demonstrated both in the soma and at the synapse. Moreover, this JNK1-mediated signaling pathway was found to inhibit AMPA-evoked calcium influx in cortical neurons and this regulation mechanism is Notch independent. Here, we provided the first evidence that Hes-1 plays an important role in synaptic function in differentiated neurons. We also identified a novel JNK1-Hes-1 signaling pathway that regulates GluR1 expression involved in synaptic function in rat cortical neurons.

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“…Çalışmalarda genel olarak doğum sonrası 9. günde 24 saat süreyle anneden ayırma ya da doğum sonrası 2-14. günler arasında 3 saat süreyle ayırma yöntemi kullanılmaktadır. Literatürde anneden ayırma yöntemlerinde bir çok farklılıklar bulunmaktadır [3][4][5] . Çalışmalarda, anne yoksunluğunun süresi, anneden ayrılma uygulanan günlerin zamanı, ayrılan yavruların cinsiyeti, mevcut yuvadan annenin uzaklaştırılması veya yavruların uzaklaştırılması yöntemleri farklılık göstermektdir.…”

Section: Anne Yoksunluğunun Deneysel Modelleriunclassified

“…Çünkü strese karşı endokrin yanıtsızlık döneminde, hipokampusta dentat girus bölgesinde hızlı hücre ölümü, oluşumu, çoğalması ve göçü arasındaki hassas dengeye düşük kortikosteron düzeylerinin aracılık ettiği ileri sürülmektedir 13 . Birçok çalışmada anne bakımının yavruda strese karşı yanıtsızlık dönemi ve HPA eksenin düzenlenmesinde kritik rol oynadığı gösterilmiştir 3,8,14 . Erken gelişim döneminde 24 saat süreyle annelerinden ayrılan yavrularda hipotalamustan CRH salınımında artma ve ardından ACTH salınımında artış olduğu, artan ACTH uyarılarının ise adrenal bezlerden glukokortikoid salınımına neden olduğu gösterilmiştir 14 .…”

Section: Anne Yoksunluğu Ve Hpa Eksen Arasındaki İlişkiunclassified

Deneysel Modellerde Anne Yoksunluğunun Beyin ve Davranışlar Üzerine Etkisi

Akıllıoğlu¹,

Karadepe²,

Binokay³

2014

Arşiv Kaynak Tarama Dergisi

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Adverse experiences in early developmental periods have significant effects on the adulthood behaviors. Maternal deprivation can be defined as prolonged loss of the care of the mother to the infant which may have intense and/or long continued even irreversible effects on the child's personality development. Maternal deprivation lead to structural, functional, neurochemical and behavioural alterations. The aim of this review is to examine the effects of maternal deprivation on the central nervous system and behavior in the light of current studies. Key words: Maternal deprivation, behaviour, central nervous system, neonatal. ÖZETErken gelişim döneminde yaşanan olumsuz deneyimler yetişkinlik dönemi davranışlarını etkilemektedir. Anne yoksunluğu, uzun süreli anne bakımından yoksun kalma sonucunda çocuğun kişilik gelişiminde geri dönüşümsüz ve şiddetli etkilerin olması şeklinde tanımlanmaktadır. Anne yoksunluğu, merkezi sinir sisteminde yapısal, hormonal, nörokimyasal ve davranışsal bir takım değişikliklere yol açmaktadır. Buyazıda, güncel çalışmalar ışığında, anne yoksunluğunun merkezi sinir sistemi ve davranışlar üzerindeki etkileri incelenmiştir. Anahtar kelimeler: Anne yoksunluğu, davranış, merkezi sinir sistemi, yenidoğan.

show abstract

Repeated maternal separation of male Wistar rats alters glutamate receptor expression in the hippocampus but not the prefrontal cortex

Cited by 107 publications

References 39 publications

Severe Life Stress and Oxidative Stress in the Brain: From Animal Models to Human Pathology

Severe Life Stress and Oxidative Stress in the Brain: From Animal Models to Human Pathology

JNK1 Inhibits GluR1 Expression and GluR1-Mediated Calcium Influx through Phosphorylation and Stabilization of Hes-1

Deneysel Modellerde Anne Yoksunluğunun Beyin ve Davranışlar Üzerine Etkisi

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