Repeated sprint exercise in hypoxia stimulates HIF-1-dependent gene expression in skeletal muscle

Nava, Roberto; McKenna, Zachary; Fennel, Zachary; Berkemeier, Quint; Ducharme, Jeremy; Magalhães, Flávio de Castro; Amorim, Fabiano T.; Mermier, Christine M.

doi:10.1007/s00421-022-04909-3

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…Previous studies reported changes in the mRNA and protein levels of GLUT4 in skeletal muscle in response to hypobaric hypoxia [9,27], as well as in athletes who performed bouts of repeated sprint exercise in hypoxic conditions [14]. Our findings contradict these later results and suggest that hypoxia superimposed on supramaximal training may blunt GLUT4 gene expression.…”

Section: Discussioncontrasting

confidence: 88%

“…Overall, RSH may improve performance through a decrease in O 2 availability, leading to an increase in HIFs and target genes at the cellular level of skeletal muscles. Among these adaptations, RSH upregulated some genes involved in glucose metabolism and mitochondrial biogenesis, depending on the training protocol [12,14,15]. Previous studies have also examined the effects of hypoxic exercise training on muscle mitochondrial turnover and metabolism in mice [16,17].…”

Section: Introductionmentioning

confidence: 99%

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Moderate Effects of Hypoxic Training at Low and Supramaximal Intensities on Skeletal Muscle Metabolic Gene Expression in Mice

Drozdovska,

Zanou,

Lavier

et al. 2023

Metabolites

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The muscle molecular adaptations to different exercise intensities in combination with hypoxia are not well understood. This study investigated the effect of low- and supramaximal-intensity hypoxic training on muscle metabolic gene expression in mice. C57BL/6 mice were divided into two groups: sedentary and training. Training consisted of 4 weeks at low or supramaximal intensity, either in normoxia or hypoxia (FiO2 = 0.13). The expression levels of genes involved in the hypoxia signaling pathway (Hif1a and Vegfa), the metabolism of glucose (Gys1, Glut4, Hk2, Pfk, and Pkm1), lactate (Ldha, Mct1, Mct4, Pdh, and Pdk4) and lipid (Cd36, Fabp3, Ucp2, Hsl, and Mcad), and mitochondrial energy metabolism and biogenesis (mtNd1, mtNd6, CytC, CytB, Pgc1a, Pgc1β, Nrf1, Tfam, and Cs) were determined in the gastrocnemius muscle. No physical performance improvement was observed between groups. In normoxia, supramaximal intensity training caused upregulation of major genes involved in the transport of glucose and lactate, fatty acid oxidation, and mitochondrial biogenesis, while low intensity training had a minor effect. The exposure to hypoxia changed the expression of some genes in the sedentary mice but had a moderate effect in trained mice compared to respective normoxic mice. In hypoxic groups, low-intensity training increased the mRNA levels of Mcad and Cs, while supramaximal intensity training decreased the mRNA levels of Mct1 and Mct4. The results indicate that hypoxic training, regardless of exercise intensity, has a moderate effect on muscle metabolic gene expression in healthy mice.

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Section: Discussioncontrasting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Moderate Effects of Hypoxic Training at Low and Supramaximal Intensities on Skeletal Muscle Metabolic Gene Expression in Mice

Drozdovska,

Zanou,

Lavier

et al. 2023

Metabolites

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“…As expected, participants exhibited marked inter- and intra-group differences on SpO 2 during RSH, with individual values ranging from 78.2 to 94.5%, allowing assessment of the relationship between hypoxemia and gains in performance. Hypothesis was made that marked hypoxemia will be associated with physiological adaptations triggered by hypoxia-inducible factor 1, which has been shown to be increased in blood and skeletal muscle after a single or several sessions of repeated-sprint exercise in hypoxia but not in normoxia ( Faiss et al, 2013 ; Brocherie et al, 2018 ; Nava et al, 2022 ; Pramkratok et al, 2022 ). Indeed, hypoxia-inducible factor 1 is known to promote the transcription of glycolytic enzymes (reviewed in ( Favier et al, 2015 )).…”

Section: Discussionmentioning

confidence: 99%

Maximizing anaerobic performance with repeated-sprint training in hypoxia: In search of an optimal altitude based on pulse oxygen saturation monitoring

et al. 2022

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Purpose: Repeated-sprint training in hypoxia (RSH) leads to great improvements in anaerobic performance. However, there is no consensus about the optimal level of hypoxia that should be used during training to maximize subsequent performances. This study aimed to establish whether such an optimal altitude can be determined and whether pulse oxygen saturation during RSH is correlated with training-induced improvement in performance.Methods: Peak and mean power outputs of healthy young males [age (mean ± SD) 21.7 ± 1.4 years] were measured during a Wingate (30 s) and a repeated-sprint ability (RSA; 10 x 6-s sprint with 24-s recovery) test before and after RSH. Participants performed six cycling sessions comprising three sets of 8 x 6-s sprint with 24-s recovery in normobaric hypoxia at a simulated altitude of either 1,500 m, 2,100 m, or 3,200 m (n = 7 per group). Heart rate variability was assessed at rest and during recovery from Wingate test before and after RSH.Results: The subjective rating of perceived exertion and the relative exercise intensity during training sessions did not differ between the three groups, contrary to pulse oxygen saturation (p < 0.001 between each group). Mean and peak power outputs were significantly increased in all groups after training, except for the mean power in the RSA test for the 3200 m group. Change in mean power on RSA test (+8.1 ± 6.6%) was the only performance parameter significantly correlated with pulse oxygen saturation during hypoxic training (p < 0.05, r = 0.44). The increase in LnRMSSD during recovery from the Wingate test was enhanced after training in the 1,500 m (+22%) but not in the two other groups (≈– 6%). Moreover, the increase in resting heart rate with standing after training was negatively correlated with SpO2 (p < 0.01, r =–0.63) suggesting that hypoxemia level during training differentially altered autonomic nervous system activity.Conclusion: These data indicate that RSH performed as early as 1,500 m of altitude is effective in improving anaerobic performance in moderately trained subjects without strong association with pulse oxygen saturation monitoring during training.

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“…The effect of exercise in a hypoxic environment on the expression level of HIF‐1α was strongly associated with increased exercise capacity. It has been shown that repetitive sprint training in a hypoxic environment increases the levels of HIF‐1α protein and mRNA, as well as the expression of a number of hypoxia‐sensitive genes downstream of HIF‐1, which is more significant for the athletes' sprint performance compared to the same training conditions under normoxic conditions 13,14 . However, there is still more controversy about whether exercise under hypoxic conditions has beneficial or detrimental effects on cardiac function and exercise capacity, thus necessitating deeper research 15 .…”

Section: Introductionmentioning

confidence: 99%

Effects of hypoxic compound exercise to promote HIF‐1α expression on cardiac pumping function, sleep activity behavior, and exercise capacity in Drosophila

Ping,

Li,

Ding

et al. 2024

The FASEB Journal

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Alteration of HIF‐1α expression levels under hypoxic conditions affects the sequence of its downstream target genes thereby producing different effects. In order to investigate whether the effect of hypoxic compound exercise (HE) on HIF‐1α expression alters cardiac pumping function, myocardial structure, and exercise capacity, we developed a suitable model of hypoxic exercise using Drosophila, a model organism, and additionally investigated the effect of hypoxic compound exercise on nocturnal sleep and activity behavior. The results showed that hypoxic compound exercise at 6% oxygen concentration for five consecutive days, lasting 1 h per day, significantly improved the cardiac stress resistance of Drosophila. The hypoxic complex exercise promoted the whole‐body HIF‐1α expression in Drosophila, and improved the jumping ability, climbing ability, moving speed, and moving distance. The expression of HIF‐1α in the heart was increased after hypoxic exercise, which made a closer arrangement of myofilaments, an increase in the diameter of cardiac tubules, and an increase in the pumping function of the heart. The hypoxic compound exercise improved the sleep quality of Drosophila by increasing its nocturnal sleep time, the number of deep sleeps, and decreasing its nocturnal awakenings and activities. Therefore, we conclude that hypoxic compound exercise promoted the expression of HIF‐1α to enhance the exercise capacity and heart pumping function of Drosophila, and improved the quality of sleep.

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Repeated sprint exercise in hypoxia stimulates HIF-1-dependent gene expression in skeletal muscle

Cited by 18 publications

References 38 publications

Moderate Effects of Hypoxic Training at Low and Supramaximal Intensities on Skeletal Muscle Metabolic Gene Expression in Mice

Moderate Effects of Hypoxic Training at Low and Supramaximal Intensities on Skeletal Muscle Metabolic Gene Expression in Mice

Maximizing anaerobic performance with repeated-sprint training in hypoxia: In search of an optimal altitude based on pulse oxygen saturation monitoring

Effects of hypoxic compound exercise to promote HIF‐1α expression on cardiac pumping function, sleep activity behavior, and exercise capacity in Drosophila

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