Repeated superovulation and surgical recovery of embryos in the ewe

Abstract

Summary. Repeated superovulation using three treatments of pFSH at intervals of 45 to 55 days during the same breeding season was carried out in 18 Prdalpes ewes.The embryos were recovered by surgery after the first two treatments and by slaughter after the last one at Day 6. Three lots of control ewes In = 5 or 6) receiving the same superovulation treatment were slaughtered respectively at the same time.The first and third repeated superovulation treatments gave averages of 5.2 + 0.6 and 4.5 ± 0.6 corpora lutea. The second one gave 3.4 t 0.4 corpora lutea, which was significantly lower than with treatments 1 and 3, but adhesions did not permit a perfect view of the whole surface of the ovaries. Introduction.

Repeated superovulation treatments in Kivircik ewes during breeding and nonbreeding seasons

“…The mean numbers of unfertilized oocytes were not significantly different among the flushing treatments (P > 0.05). Repeated surgical recovery caused the development of adhesions in some ewes and thus hindered oocyte capture (16). In the present study, the increased number of unfertilized oocytes in the nonbreeding season treatment could be attributed to impaired sperm transport due to adhesions or to an increased number of immature oocytes.…”

In vivo embryo production in some native goat breeds in Turkey

“…The use of intraabdominal heparinized washing solutions is thought to have a positive effect on RR and TEs. In the NO group, the formation of postoperative adhesions may have decreased the number of TEs recovered, especially adhesions in the uterine lumen (27,28).…”

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

“…On the other hand, findings in small ruminants make clear that surgical embryo collection negatively affects future fertility [13][14]. Moreover, repeated surgical recovery reduced ovulation and fertilization rates as well as the number of embryos recovered in sheep and goats [15][16][17]. These findings indicate that the procedure for embryo collection contains elements that diminish ovulation, fertilization rates or numbers of embryos pursuing full development.…”

“…Surgery implies trauma, of various intensities, to the tip of the uterus (perforation of the wall) as well as manipulation for ovarian/salpingeal/uterine retrieval and exposure, which may cause bleeding and eventual adhesions post-surgery [12]. In small ruminants, post-operative adhesions have been associated with a reduced capture of oocytes, decreased ovulation rate and lower embryo recovery [15][16][17], all of which compromise future reproductive outcome for the donor. These findings, if extrapolated to pigs, could explain the lack of effect on farrowing rates and the reduced litter size obtained in our study for donor sows following surgical embryo collection.…”