Repellency activity of vanillyl butyl ether is mediated by transient receptor potential vanilloid channels in the red flour beetle, Tribolium castaneum (Herbst)

Shimomura, Kenji; Oikawa, Hinoki; Kayukawa, Takumi; Asamizu, Satoshi; Suzuki, Nobuhiro; Yajima, Seishi; Tomizawa, M.

doi:10.1016/j.aspen.2019.07.015

Cited by 2 publications

(3 citation statements)

References 33 publications

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“…In the contact repellency system, the tarsal leg segments of the mosquito Aedes aegypti mediated DEET detection, but the chemoreceptor for this process remains unknown 11 . In our previous experiment, the RNAi treatment of TcNan and TcIav , members of the TRPV channels of T. castaneum did not affect the repellent activity of DEET in T. castaneum 40 . From these results, we used the same methodology for RNAi treatment of TcTRPM .…”

Section: Discussionmentioning

confidence: 91%

“…11 In our previous experiment, the RNAi treatment of TcNan and TcIav, members of the TRPV channels of T. castaneum did not affect the repellent activity of DEET in T. castaneum. 40 From these results, we used the same methodology for RNAi treatment of TcTRPM. We discovered that contact repellent activities against L-menthol and MPD were mediated specifically by the TcTRPM channel, which differed from DEET detection.…”

Section: Discussionmentioning

confidence: 99%

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Contact repellency by l‐menthol is mediated by TRPM channels in the red flour beetle Tribolium castaneum

Shimomura

Oikawa

Hasobe

et al. 2020

Pest Management Science

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BACKGROUND Among insects, beetles are one of the most destructive pests of agricultural and stored products. Researchers have been investigating alternatives to pesticides for more sustainable pest management. Here, we focused on insect transient receptor potential (TRP) channel‐targeted repellency. Among transient receptor potential melastatin (TRPM) channels, mammalian TRPM8 is activated by menthol and its derivatives, but few previous studies have reported on whether the insect TRPM channel is activated by chemical compounds. Here, we investigated whether the TRPM channel (TcTRPM) of the red flour beetle Tribolium castaneum (Herbst), a major stored‐products pest, mediated the repellent behavior of l‐menthol and its derivatives. RESULTS We initially investigated the repellent activity of l‐menthol and menthoxypropanediol (MPD) against T. castaneum. The laboratory bioassay revealed that the repellent activities of l‐menthol and MPD were dose dependent. RNA interference was used for transcriptional knockdown of TcTRPM and revealed that a reduced transcript level resulted in a significant decrease in l‐menthol and MPD repellent activities. However, no significant decrease was observed for N,N‐diethyl‐3‐methylbenzamide (DEET) repellency. The most abundant TcTRPM transcripts were observed in the antennae. However, antennae‐plucked beetles maintained their repellent behavior with l‐menthol. CONCLUSION The repellent activities of l‐menthol and MPD for T. castaneum are mediated by TcTRPM, and it was suggested that the olfactory response is not adequate for avoidance, but that contact repellency might be a more important repellant method. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Contact repellency by l‐menthol is mediated by TRPM channels in the red flour beetle Tribolium castaneum

Shimomura

Oikawa

Hasobe

et al. 2020

Pest Management Science

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“…DEETbased repellency involves multiple pathways and mechanisms; however, it remains an area of contention 30 . In our prior studies, T. castaneum showed repellency behavior for DEET 29,31 ; however, to the best of our knowledge, www.nature.com/scientificreports/ there are no studies that report that DEET activates insect TRP channels. We analyzed the area-preference test with TcTRPA1 dsRNA treatments for DEET repellency, and the repellent index of the TcTRPA1 dsRNA-treated beetles did not show a significant difference from that of the untreated beetles, implying the specific interaction of citronellal against TcTRPA1.…”

Section: Discussionmentioning

confidence: 94%

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Shimomura

Ino

Tamura

et al. 2022

Sci Rep

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The sensory perception of irritant chemicals results in escape and repellency behavioral patterns in insects. Transient receptor potential channels are cation channels that function as sensor proteins for several types of signals, such as light, sound, temperature, taste, as well as chemical and physical stimuli; among these, the TRPA channel is widely conserved and activated by irritant chemicals. Certain plant-derived essential oils (EOs), produced by secondary metabolism, are mixtures of volatile compounds, which are used as repellents because they contain environmentally sustainable ingredients. Citronellal, which is present in citronella EO from Cymbopogon species, is a potentially viable insect repellent; however, the repellency capability against coleopteran beetles remains limited. We investigated the citronellal-derived repellency behavior for the red flour beetle Tribolium castaneum, in which TcTRPA1 and odorant receptor co-receptor (Orco) expressions were mediated by RNA interference. Area-preference tests showed dose-dependent repellency behavior for citronellal; additionally, both TcTRPA1 and TcOrco double-strand RNA (dsRNA) micro-injection showed clear TcTRPA1 and TcOrco transcript reductions, and only TcTRPA1 dsRNA treatment significantly impaired repellency behavior. The relative expression level of the TcTRPA1 transcripts, evaluated by quantitative reverse-transcription PCR (qRT-PCR), revealed dominant expression in the antennae, indicating the antennae-expressed TcTRPA1-mediated repellency behavior.

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Repellency activity of vanillyl butyl ether is mediated by transient receptor potential vanilloid channels in the red flour beetle, Tribolium castaneum (Herbst)

Cited by 2 publications

References 33 publications

Contact repellency by l‐menthol is mediated by TRPM channels in the red flour beetle Tribolium castaneum

Contact repellency by l‐menthol is mediated by TRPM channels in the red flour beetle Tribolium castaneum

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

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