Replacement of Chloroform Throughout Glycosphingolipid Isolation

Properties, analysis and purification of milk polar lipids

“…The disadvantage is, however, that coextraction of non-lipid contaminants can occur, which can give rise to erroneous results, for example, when calculating the gravimetric difference after evaporation of the solvent, or when measuring total inorganic phosphorous. Evaluations, modifications and alternatives of several methods for the extraction of polar lipids were done by Theodet and Gandemer (1991), Shaikh (1994), Vaghela and Kilara (1995a) and Heitmann, Lissel, Kempken, and Muthing (1996). Prior to analysis, a preconcentration step is often performed using open column chromatography or small solid-phase extraction (SPE) cartridges.…”

Tandem Mass Spectrometry of Sphingolipids: Application in Metabolic Studies and Diagnosis of Inherited Disorders of Sphingolipid Metabolism

“…Cells and tissues are homogenized, and aliquots of homogenate are usually taken for protein determination. Many extraction procedures have been introduced over the years, most of which have been based on chloroform-methanol mixtures (Bligh & Dyer, 1959;Folch, et al, 1957) or less harmful solvents such as 2-propanol, ethylacetate, hexane or tetrahydrofuran (Heitmann, et al, 1996). The first approach remains the most popular and efficient.…”

Affinity Enrichment of Bovine Lactoferrin in Whey

“…11 The sample was centrifuged at 11,000 x g and the supernatant was collected and evaporated under nitrogen gas. The total amount of gangliosides purified was determined by the periodate-resorcinol method using sialic acid as standard according to Jourdian et al 12 …”

“…17 Gangliosides were purified from freeze-dried lactose-free buttermilk powder using a mixture consisting of chloroform, methanol, and water. 11 The gangliosides were soluble in the organic phase and the proteins and residual lactose were removed with the water phase. The organic extraction of buttermilk decreased the gangliosides content by 72%, but also decreased the protein level to 1 mg/L and the lactose to 6.6 mg/L.…”