Replacement of Muller-Kauffmann's tetrathionate brilliant green bile broth by Rappaport-Vassiliadis' magnesium chloride malachite green broth in the standard method for the detection of salmonellae

Beckers, H. J.; Roberts, Diane; Pietzsch, O.; Schothorst, M. van; Vassiliadis, P.; Kampelmacher, E. H.

doi:10.1016/0168-1605(87)90059-6

Cited by 23 publications

(4 citation statements)

References 8 publications

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“…1981). Van Schotthorst et al (1987) recommended enrichment in Rappaport-Vassiliadis (RV) broth at 43"C, and Beckers et al (1987b) proposed replacing the Tetrathionate method with the RV method in the IS0 standard (Anon. 1990).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of different enrichment and isolation procedures for the detection of Salmonella serotypes in edible offal

Arroyo¹,

Arroyo

1995

Journal of Applied Bacteriology

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Rapid detection systems for Salmonella in foodstuffs are currently being developed. However, existing standards still call for application of traditional methods employing pre-enrichment followed by selective enrichment and isolation. The efficacy of various methods was tested using 264 chicken and lamb organ meats. Pre-enrichment was carried out in Tryptone Soy Broth (TSB) and enrichment in Tetrathionate Brilliant Green Broth (TTB) at 37 degrees C, Selenite Broth with Brilliant Green and Sulphapyridine at 37 degrees C and 43 degrees C, and Rappaport-Vassiliadis Broth (RV 10) at 42 degrees C. The isolation media were Brilliant Green Agar (BGA), Deoxycholate Citrate Agar, Hektoen Enteric Agar (HEA) and Salmonella-Shigella Agar. Enrichment in RV/42 degrees C followed by isolation on BGA as recommended by ISO standard no. 6579 and enrichment in TTB/37 degrees C followed by isolation in HEA, no longer recommended by that standard, produced the best results. Low percentages of positive samples and difficulties in detecting Salmonella are the result of interference by competing organisms (Enterobacteriaceae) and the number of salmonellas present after enrichment. A total of 528 samples (TSB, eggs, lamb liver and chicken liver) were inoculated with Salm. enteritidis, Salm. kapemba and Salm. virchow, and the preceding experiment was repeated. All the TSB and egg samples tested positive, but the percentage of positive samples from the lamb and chicken liver was only 81-92%. Recovery of the salmonellas did not depend upon the method employed or the serotype inoculated but instead on interference by competing flora and the numbers of Salmonella present in the samples.

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Section: Introductionmentioning

confidence: 99%

Efficiency of different enrichment and isolation procedures for the detection of Salmonella serotypes in edible offal

Arroyo¹,

Arroyo

1995

Journal of Applied Bacteriology

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“…In general, each of these phases takes 24 h to perform, giving a test time of 72-96 h. There are alternative methods, which have been designed to reduce this time, most of which use an alternative to the isolation of colonies on selective plates (Fitts et al 1983;Beckers et al 1987;Easter and Gibson 1985;Lee et al 1990). However, whilst these methods are useful in screening out negative samples, positive results require a return to conventional cultural isolation for confirmation.…”

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confidence: 99%

Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection

Mansfield

Forsythe

1993

Lett Appl Microbiol

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One hundred and twenty foodstuffs were tested for the enrichment of Salmonella species by immunoseparation. The foodstuffs covered six groups: raw chicken, prawns, skimmed milk powder, herbs and spices, cocoa powder and animal feed. Half of the food samples were spiked with one Salmonella species: Salm. ealing, Salm. enteritidis, Salm. give, Salm. typhimurium or Salm. virchow. Comparison of Salmonella recovery with standard methods (selenite cystine broth, tetrathionate broth and Rappaport‐Vassiliadis broth) was carried out. Immunoseparation gave similar numbers of true positives to the standard enrichment methods in a short time period. Only immunoseparation isolated Salmonella species from spiked garlic granules demonstrating the possible recovery of sublethally injured cells.

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“…The results also show that the Rappaport Vassiliadis (RV) medium (11) was more efficient than the TBB. This means that the isolation method must be improved further (5). Table 4 summarises the strategy recommended for standardisation of microbiological methods ensuing from 20 years experience with Salmonella isolation procedures.…”

Section: Minced Meat Studiesmentioning

confidence: 99%

Standardisation of microbiological methods

Schothorst

1987

Veterinary Quarterly

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Replacement of Muller-Kauffmann's tetrathionate brilliant green bile broth by Rappaport-Vassiliadis' magnesium chloride malachite green broth in the standard method for the detection of salmonellae

Cited by 23 publications

References 8 publications

Efficiency of different enrichment and isolation procedures for the detection of Salmonella serotypes in edible offal

Efficiency of different enrichment and isolation procedures for the detection of Salmonella serotypes in edible offal

Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection

Standardisation of microbiological methods

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