Repletion of antioxidant status by EGCG and retardation of oxidative damage induced macromolecular anomalies in aged rats

Kumaran, Vadivel Senthil; Arulmathi, Karpagavinayagam; Srividhya, Ravichandran; Kalaiselvi, Periandavan

doi:10.1016/j.exger.2007.10.017

Cited by 71 publications

(33 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…30) Recently, Senthil et al have reported the antioxidant effects of EGCG in aged rats. 31) In addition, EGCG can protect several organs from lipid peroxidation injury. However, there was no previous report on the protective effects of EGCG against D-gal-induced brain injury in mice.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of (-)-Epigallocatechin-3-gallate on Aging Mice Induced by D-Galactose

Zhao

Wei

et al. 2009

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Aging is a complicated multifactorial process in which a progressive decline in the physiologic function of organisms occurs. Oxidative stress and reactive oxygen species (ROS) have been proposed to be major cause of aging and other aging-related neurodegenerative conditions such as Alzheimer's disease.1,2) Aging-related oxidative damage in the brain and memory impairment mainly result from an imbalance between ROS generation and antioxidant enzyme activities. The overproduction of ROS or decrease in antioxidants in the brain can cause lipid peroxidation, nuclear and mitochondrial DNA damage, and protein oxidation and finally affect the normal functions of organisms. [3][4][5][6] D-Galactose (D-gal) is a reducing sugar normally present in the body. When present at levels greater than its normal content, it can be oxidized into aldehydes and hydrogen peroxide (H 2 O 2 ) by galactose oxidase. 7,8) It has been shown that D-galtreated animal models show aging-related changes including the impairment of spatial learning and memory, object novelty preference, and locomotor activity, 9,10) and increased production of ROS and lowered activities of antioxidant enzymes. 11,12) Other studies demonstrated that long-term subcutaneous injection of D-gal in mice induced decreased immune responses and increased cell karyopyknosis, apoptosis, and caspase-3 protein levels in hippocampal neurons.11,13) Therefore mice continuously injected with D-gal have been extensively used for pharmacologic research on brain aging.(Ϫ)-Epigallocatechin-3-gallate (EGCG) is a member of the catechin family and a major polyphenolic constituent of green tea.14) It was reported that EGCG has potent ironchelating, antioxidant, antiinflammatory, anticancer, and neuroprotective activities. [15][16][17][18][19] In particular, EGCG has been shown to have neuroprotective effects by elevating the a-secretase activity of amyloid precursor protein (APP) and conversion to soluble APP-alpha (sAPP-a) and reducing amyloid beta (Ab)-induced neurotoxicity in a 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) mouse model, the "Swedish" APP transgenic (Tg2576) mouse model of Alzheimer's disease, and N2a cells stably transfected with "Swedish" mutant human APP. 20,21) Furthermore, some studies have recently demonstrated that the neuroprotective mechanisms of EGCG are partly due to increasing activities of antioxidant enzymes and decreasing advanced glycation endproduct (AGE)-induced damage in aged rat brain or neuronal cells. 22,23) However, no study has been performed to evaluate whether EGCG has protective effects on aging mice induced by D-gal and its mechanisms of action. In the present study, we induced the aging mice model with D-gal (150 mg/kg/d, 6 weeks subcutaneously (s.c.)) and observed whether EGCG (2 mg/kg/d or 6 mg/kg/d, 4 weeks intragastrically (i.g.)) had potent antioxidant and antiapoptotic neuroprotective effects using behavioral testing and measurements of the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), con...

show abstract

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of (-)-Epigallocatechin-3-gallate on Aging Mice Induced by D-Galactose

Zhao

Wei

et al. 2009

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…EGCG decreased the hepatic levels of lipid peroxides (50% decrease) and protein carbonyls (39% decrease) [38]. EGCG treatment also increased the hepatic levels of both small moecule antioxidants and antioxidant enzymes compared to control rats.…”

Section: Role Of Antioxidant Effects In the Cancer Preventive Activitmentioning

confidence: 99%

The antioxidant and pro-oxidant activities of green tea polyphenols: A role in cancer prevention

Lambert

Elias

2010

Archives of Biochemistry and Biophysics

745

512

View full text Add to dashboard Cite

Green tea (Camellia sinensis) is rich in catechins, of which (−)-epigallocatechin-3-gallate (EGCG) is the most abundant. Studies in animal models of carcinogenesis have shown that green tea and EGCG can inhibit tumorigenesis during the initiation, promotion and progression stages. Many potential mechanisms have been proposed including both antioxidant and pro-oxidant effects, but questions remain regarding the relevance of these mechanisms to cancer prevention. In the present review we will discuss the redox chemistry of the tea catechins and the current literature on the antioxidant and pro-oxidative effects of the green tea polyphenols as they relate to cancer prevention. We report that although the catechins are chemical antioxidants which can quench free radical species and chelate transition metals, there is evidence that some of the effects of these compounds may be related to induction of oxidative stress. Such pro-oxidant effects appear to be responsible for the induction of apoptosis in tumor cells. These pro-oxidant effects may also induce endogenous antioxidant systems in normal tissues that offer protection against carcinogenic insult. This review is meant point out understudied areas and stimulate research on the topic with the hope that insights into the mechanisms of cancer preventive activity of tea polyphenols will result.

show abstract

“…Administering EGCG to aged rats reduced oxidative stress, and the EGCG-treated animals had decreased levels of lipid peroxidation and protein carbonylation, as well as increased levels of antioxidants and antioxidant enzymes in the liver, skeletal muscle, and brain [12,13]. Also, supplementation of tea catechins (500 mg/day) in the diets of healthy human volunteers for 4 weeks resulted in an 18 % decrease in plasma oxidized low-density lipoprotein compared with the control [14].…”

Section: Egcg As An Antioxidant or A Pro-oxidantmentioning

confidence: 92%

Polyphenols in Health and Disease

Zhang

2015

Cell Biochem Biophys

View full text Add to dashboard Cite

Deadly diseases, such as cardiovascular diseases and cancer, remain the major health problems worldwide. Research in cardiovascular diseases and genome-wide association studies were successful in indentifying the gene loci associated with these threatening diseases. Yet, a substantial number of casual factors remain unexplained. Over the last decade, a better understanding of molecular and biochemical mechanisms of cardiac diseases led to developing a rationale for combining various protective agents, such as polyphenols, to target multiple signaling pathways. The present review article summarizes recent advances of the use of polyphenols against diseases, such as cardiac diseases.

show abstract

Repletion of antioxidant status by EGCG and retardation of oxidative damage induced macromolecular anomalies in aged rats

Cited by 71 publications

References 52 publications

Neuroprotective Effects of (-)-Epigallocatechin-3-gallate on Aging Mice Induced by D-Galactose

Neuroprotective Effects of (-)-Epigallocatechin-3-gallate on Aging Mice Induced by D-Galactose

The antioxidant and pro-oxidant activities of green tea polyphenols: A role in cancer prevention

Polyphenols in Health and Disease

Contact Info

Product

Resources

About