Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo

Bosselman, R A; Hsu, Rou-Yin; Boggs, Tina; Hu, Sylvia; Bruszewski, Joan; Ou, Susan; Souza, Lawrence M.; Kozar, Leland G.; Martin, Francis H.; Nicolson, Margery

doi:10.1128/jvi.63.6.2680-2689.1989

Cited by 51 publications

(9 citation statements)

References 41 publications

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“…The most successful method for generating transgenic birds has been the use of retroviral vectors (Salter et al, 1987; Petitte and Mozdziak, 2002). Bosselmann et al (1989a,b, 1990) generated transgenic lines of chickens by using a replication‐defective reticuloendotheliosis virus to overexpress the chicken growth hormone gene in embryos. Transgenic birds carrying the lacZ gene have been produced by infecting primordial germ cells with a replication‐defective spleen necrosis‐derived retroviral vector encoding the lacZ gene and transplanting the transformed primordial germ cells into early recipient embryos (Vick et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Development of transgenic chickens expressing bacterial β‐galactosidase

Mozdziak

Borwornpinyo

McCoy

et al. 2003

Developmental Dynamics

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Replication-defective retroviral vectors are efficient vehicles for the delivery of exogenous genes, and they may be used in the generation of transgenic animals. The replication-defective retroviral SNTZ vector carrying the lacZ gene with a nuclear localized signal was injected into the subgerminal cavity of freshly laid eggs. Subsequently, the eggs were allowed to hatch, and the chickens were screened for the lacZ gene by using the polymerase chain reaction. Eight of 15 male chickens that survived to sexual maturity contained the lacZ gene in their semen. Subsequently, these males were mated with wild-type female chickens. From one of the eight lacZ-positive G 0 males, two lacZ-positive male chickens were produced from a total of 224 G 1 progeny for a germline transmission rate of 0.89%. Both G 1 male chickens carrying the lacZ gene were mated with wild-type female chickens and 46.5% of the G 2 progeny contained the lacZ gene, which is consistent with the expected Mendelian 50% ratio for a heterozygous dominant allele. The product of the lacZ gene, nuclear localized ␤-galactosidase, was expressed in primary myoblast cultures derived from G 2 chickens, and it was also expressed in whole G 2 chicken embryos. Developmental Dynamics 226:439 -445, 2003.

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Section: Introductionmentioning

confidence: 99%

Development of transgenic chickens expressing bacterial β‐galactosidase

Mozdziak

Borwornpinyo

McCoy

et al. 2003

Developmental Dynamics

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“…An exogenous gene was first successfully introduced into the chicken genome using a retroviral vector to produce transgenic chickens (Salter et al . 1986; Bosselman et al . 1989a,b).…”

Section: Introductionmentioning

confidence: 99%

Detection of the EGFP sequence in breast muscle of 3‐year‐old chicken after transfection using sonoporation

Matsubara

Tagami

Matsunaga

et al. 2011

Animal Science Journal

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In our continuing effort to generate transgenic chickens, sonoporation was chosen to insert an exogenous gene into the chicken genome. An EGFP expression vector (pCAG-EGFPac) and microbubbles were injected into the central disc of stage-X blastoderm or the germinal crescent of stage-4 embryos, followed by ultrasonic vibration. Nineteen chicks out of 108 treated embryos hatched, six females and six males out of these 19 chicks grew to sexual maturity and two females and three males lived for 3 years. Genomic DNA from 17 out of 35 gonads from embryos and chicks that died before sexual maturity was EGFP-positive by PCR. No EGFP sequence was detected in the genomic DNA of 322 embryos from six sexually mature females and the semen from four sexually mature males by PCR. When genomic DNA was obtained from various tissues of five 3-year-old chickens, the EGFP sequence was amplified from the genomic DNA of the breast muscle of a female (No. 85). The above sequence was subjected to DNA sequencing and verified to be the EGFP sequence. These results showed that sonoporation is an effective tool for the transduction of exogenous genes into chicken embryos for the generation of transgenic chickens.

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“…1. Строение SNV (вирус некроза селезенки) и ретровирусных векторов, созданных на его основе: а -строение ретрови русов на примере SNV [5]; б -сплайсирующийся вектор pjD216 NeoHy [5]; в -вектор с внутренним промотором рМЕ 111 [16]; г -бицистронный вектор с IRES-последовательностя ми pjD214HyBi [5]; gag, рої, env-гены, кодирующие вирус ные белки; LTR -длинные концевые повторы; U3 -уникаль ный участок LTR; R -терминальный повтор; attR-attL -тер минальный сайт прикрепления (правый и левый); pbs -праймер-связывающий сайт; sd -донорный и sa -акцепторный сайты сплайсинга; ppt -полипуриновый тракт;…”

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“…В репликативно-дефектном векторе кодирую щая область заменена на «транспортируемую» ДНК, маркерные гены и гены устойчивости к антибиотикам для селекции в культуре клеток. В качестве «транспортируемой» ДНК используют как небольшие фрагменты генома (ген a-актина ске летных мышц курицы [13,14]), так и кДНК последовательности [15,16]. Преимуществом по следних является их небольшой размер, способ ность транскрибироваться с промотора в 5-LTR Рис.…”

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“…Чтобы усилить транскрипцию второго гена, его помещают под собственным внутренним промото ром. В качестве внутреннего промотора чаще всего используют Simian virus 40 (SV40) и тимидинкиназный промотор вируса герпеса (HSV) [16,[25][26][27][28]. Вектор этого типа М 111 (рис.…”

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