Replication in Polytene Chromosomes

Rudkin, George T.

doi:10.1007/978-3-540-37164-9_3

Cited by 80 publications

(25 citation statements)

References 112 publications

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“…Chromosomal subdivision 89B, which does not contain heat shock genes, remains H4 acetylated during heat shock. lated H3 in polytene chromosomes, rather than isolation of phosphorylated H3 from whole cell extracts, gives us the advantage of examining phosphorylation of H3 in a nonmitotic environment (Rudkin 1972). To examine whether the heat shock-induced puffs contain N-terminal phosphorylated H3 molecules, polytene chromosomes were stained with antibodies specific for Ser 10 phosphorylated histone H3 (Hendzel et al 1997).…”

Section: Distribution Of Phosphorylated Histone H3 Changes In Responsmentioning

confidence: 99%

Phosphorylation of histone H3 correlates with transcriptionally active loci

Nowak¹,

Corcés²

2000

Genes Dev.

209

139

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Posttranslational modifications of the N-terminal tails of the core histones within the nucleosome particle are thought to act as signals from the chromatin to the cell for various processes. The experiments presented here show that the acetylation of histones H3 and H4 in polytene chromosomes does not change during heat shock. In contrast, the global level of phosphorylated H3 decreased dramatically during a heat shock, with an observed increase in H3 phosphorylation at the heat shock loci. Additional experiments confirm that this change in phosphorylated H3 distribution is dependent on functional heat shock transcription factor activity. These experiments suggest that H3 phosphorylation has an important role in the induction of transcription during the heat shock response.

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Section: Distribution Of Phosphorylated Histone H3 Changes In Responsmentioning

confidence: 99%

Phosphorylation of histone H3 correlates with transcriptionally active loci

Nowak¹,

Corcés²

2000

Genes Dev.

209

139

View full text Add to dashboard Cite

show abstract

“…6, 9) but at early stage chromosome morphology is not clear for all com plements. In this respect the polytene chromosomes of this taxon are differing from the dipteran giant chromosomes where under-replication of the heterochromatin is found associated usually (Rudkin 1972) and no satellite bands of salivary gland DNA at the usual loadings of the ultracentrifuge cell could be found (Gall et al 1974). Heterochromatin is considered as the site of satellite DNA in the chromo somes.…”

Section: Resultsmentioning

confidence: 89%

Giemsa-banding pattern of chromosomes in Phaseolus vulgaris L.

Bhattacharya¹

1978

CYTOLOGIA

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“…Pericentric heterochromatin is almost completely underreplicated in polytene chromosomes of Drosophila (Rudkin, 1965;Berendes and Keyl, 1967;Mulder et al, 1968). Unexpectedly, in otu mutant NC chromosomes, blocks of banded material were present in the heterochromatin region (Mal'ceva and Zhimulev, 1993).…”

Section: Discussionmentioning

confidence: 97%

“…For instance, euchromatin passes up to 10 replication cycles in salivary glands (SGs), whereas heterochromatin passes only 1-2 cycles (Rudkin, 1965;Berendes and Keyl, 1967;Mulder et al, 1968). However, the number of cycles can vary significantly in different heterochromatic regions.…”

mentioning

confidence: 99%

Abnormal tissue-dependent polytenization of a block of chromosome 3 pericentric heterochromatin inDrosophila melanogaster

Koryakov

Domanitskaya

Belyakin

et al. 2003

Journal of Cell Science

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Heterochromatic DNA sequences in the polytene chromosomes of Drosophila melanogaster salivary glands are under-replicated in wild-type strains. In salivary glands of SuUR and in the nurse cells of otumutants, under-replication is partly suppressed and a banded structure appears within the centric heterochromatin of chromosome 3. This novel banded structure in salivary gland chromosomes was called Plato Atlantis. In order to characterize the heterochromatic component of Plato Atlantis, we constructed a fine-scale cytogenetic map of deletions with break points within centric heterochromatin (Df(3L)1-16, Df(3L)2-66, Df(3R)10-65, Df(3R)4-75 and Df(3L)6B-29 + Df(3R)6B-29). Salivary gland chromosomes show that Df(3L)1-16 removes the complete Plato Atlantis, while Df(3L)2-66 deletes the most proximal 3L regions. These deletions therefore show a substantial cytological overlap. However, in the chromosomes of nurse cells, the same deficiencies remove distinct heterochromatic blocks,with the region of overlap being almost invisible. Satellite (AATAACATAG,AAGAG) and dodecasatellite DNAs mapped in a narrow interval in salivary glands but were found in three clearly distinguishable blocks in nurse cells. The 1.688 satellite was found at a single site in salivary glands but at two sites in nurse cells. We show that newly polytenized heterochromatic structures include blocks h47-h50d of mitotic heterochromatin in salivary glands, but the additional blocks h50p, h53 and h57 are also included in nurse cell chromosomes. Tissue specificity of the patterns of abnormal heterochromatic polytenization implies differential control of DNA replication in somatic and germline cells.

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Replication in Polytene Chromosomes

Cited by 80 publications

References 112 publications

Phosphorylation of histone H3 correlates with transcriptionally active loci

Phosphorylation of histone H3 correlates with transcriptionally active loci

Giemsa-banding pattern of chromosomes in Phaseolus vulgaris L.

Abnormal tissue-dependent polytenization of a block of chromosome 3 pericentric heterochromatin inDrosophila melanogaster

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