Replication kinetics of duck virus enteritis vaccine virus in ducklings immunized by the mucosal or systemic route using real-time quantitative PCR

Qi, Xuefeng; Yang, Xiaoyan; Cheng, Anchun; Wang, Mingshu; Guo, Yufei; Jia, Renyong

doi:10.1016/j.rvsc.2008.05.001

Cited by 22 publications

(21 citation statements)

References 12 publications

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“…Hence, the development of rapid methods for detection of the strain from clinical samples is important for pathogenic diagnosis and disease control. The dynamic quantitative detection of viral loads in tissues is useful for understanding the pathogenicity of viruses and determination of the target tissues collected for clinical virus detection (17,18). The results showed that high DHAV-C loads were detected in various tissues and organs at 24 h postinfection and suggested that DHAV-C is a pantropic virus and causes extensive tissue damage in ducklings.…”

Section: Discussionmentioning

confidence: 99%

Development of a Real-Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C

et al. 2012

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Recently, duck hepatitis A virus genotype C (DHAV-C), a causative agent of duck viral hepatitis, has been responsible for increasing economic losses in the duck industry in China and South Korea. In this study, a real-time PCR assay targeting the 2C gene for detecting DHAV-C was developed. The assay was confirmed to be specific and sensitive, and the minimum detection limit was 3.36 ؋ 10 3 copies per reaction, making this assay suitable for rapid diagnosis of DHAV-C infection from clinical samples. In addition, the dynamics of the viral loads in tissues of specific-pathogen-free (SPF) ducklings infected with DHAV-C were investigated using this method. The DHAV-C could be detected earliest in the liver within 12 h postinfection. Moreover, high viral loads were identified in the heart, liver, spleen, lung, kidney, bursa of Fabricius, thymus, pancreas, brain, and small intestine after 24 h postinfection. Taking the data collectively, the study described in this report is the first to have developed a realtime PCR method for detection of DHAV-C and thus contributes to pathogenicity research.

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Section: Discussionmentioning

confidence: 99%

Development of a Real-Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C

et al. 2012

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“…Currently, the virus is stored in the Avian Disease Research Center of the Sichuan Agricultural University of China (21). The DPV attenuated vaccine strain CHa is a modified live commercial vaccine (China Animal Husbandry Industry Co. Ltd.) that was the product of a DPV CHv isolate cultured in chicken embryo fibroblasts (CEF) for 80 serial passages (17).…”

Section: Methodsmentioning

confidence: 99%

“…The kinetics of the attenuated DPV vaccine strain CHa determined that subcutaneous administration had a larger effect on the vaccine virus distribution in tissue than the oral and nasal routes of administration did (17). The growth kinetics, back passages, residual virulence, excretion, and seroconversion of this strain have been investigated thoroughly (17)(18)(19). Briefly, the attenuated virus has a broad tissue tropism.…”

mentioning

confidence: 99%

An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection

Huang

Jia

Wang

et al. 2014

Clin Vaccine Immunol

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c Duck plague (DP) is a severe disease caused by DP virus (DPV). Control of the disease is recognized as one of the biggest challenges in avian medicine. Vaccination is an efficient way to control DPV, and an attenuated vaccine is the main routine vaccine. The attenuated DPV vaccine strain CHa is a modified live vaccine, but the systemic and mucosal immune responses induced by this vaccine have been poorly understood. In this study, the immunogenicity and efficacy of the vaccine were evaluated after subcutaneous immunization of ducks. CD4؉ and CD8 ؉ T cells were counted by flow cytometry, and humoral and mucosal Ig antibodies were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that high levels of T cells and Ig antibodies were present postimmunization and that there were more CD4 ؉ T cells than CD8 ؉ T cells. Titers of humoral IgG were higher than those of humoral IgA. Local IgA was found in each sample, whereas local IgG was found only in the spleen, thymus, bursa of Fabricius, harderian gland, liver, bile, and lung. In a protection assay, the attenuated DPV vaccine completely protected ducks against 1,000 50% lethal doses (LD 50 ) of the lethal DPV strain CHv via oral infection. These data suggest that this subcutaneous vaccine elicits sufficient systemic and mucosal immune responses against lethal DPV challenge to be protective in ducks. This study provides broad insights into understanding the immune responses to the attenuated DPV vaccine strain CHa through subcutaneous immunization in ducks.

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“…Currently, both conventional and quantitative PCR have been employed for the detection of DEV (Guo et al 2006;Yang et al 2006;Qi, Yang, Cheng, Wang, Guo, and Jia 2009). Beside diagnostics, the rapidity, sensitivity and specificity of these assays could provide a suitable tool towards understanding the epizootology of DP (Hansen et al 1999;Pritchard et al 1999;Hansen et al 2000;Sandhu & Shawky 2003;Cheng et al 2004).…”

Section: Molecular Diagnosis Of Devmentioning

confidence: 99%

“…Beside diagnostics, the rapidity, sensitivity and specificity of these assays could provide a suitable tool towards understanding the epizootology of DP (Hansen et al 1999;Pritchard et al 1999;Hansen et al 2000;Sandhu & Shawky 2003;Cheng et al 2004). DEV-specific DNA segments can be amplified from infected cell culture supernatant and tissues from esophagus, liver, kidney and spleen (Plummer et al 1998;Thayer & Beard 1998;Hansen et al 1999Hansen et al , 2000Pritchard et al 1999;Yang et al 2005;Xuefeng et al 2008;Liu et al 2009;Qi, Yang, Cheng, Wang, Guo, and Jia 2009;Wu et al 2011b;Lin et al 2013). Plummer et al (1998) diagnosed the DEV by targeting the highly conserved domain of the UL6 gene.…”

Section: Molecular Diagnosis Of Devmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

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Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%-100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.

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Replication kinetics of duck virus enteritis vaccine virus in ducklings immunized by the mucosal or systemic route using real-time quantitative PCR

Cited by 22 publications

References 12 publications

Development of a Real-Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C

Development of a Real-Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C

An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection

Duck virus enteritis (duck plague) – a comprehensive update

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