Replication of 5 S ribosomal genes precedes the appearance of early nuclear replication complexes

Cuadrado, Ángeles; Pelayo, Helvia R.; Giménez-Abián, M. I.; Jouve, N.; Torre, Consuelo de la

doi:10.1016/s0171-9335(98)80113-1

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…Mitotic cells were transformed into binucleates by a caffeine treatment in telophase that prevents the fusion of the Golgi vesicles in the cytokinetic plate. These cells maintain a symmetrical distribution of sister centromeres and telomeres in both nuclei, similar to that produced during sister-chromosome segregation in ana-telophase (Cuadrado et al 1998). After a 2-h-long caffeine treatment that labelled the telophases as binucleates, and a later 2-h recovery, cells were synchronised with HU and fed with BrdU, as in the previous experiments (Fig.…”

Section: Nuclear Polarisation Of Replication Focisupporting

confidence: 69%

“…In plant cells the activation of replication clusters occurs with a strict topological and temporal organisation (Kakeda and Yamagata 1992;Sparvoli et al 1994;Cuadrado et al 1998;Jasencakova et al 2000Jasencakova et al , 2001, as is the case in yeast and animal cells (Ma et al 1998;Leonhardt et al 2000;Gilbert 2001;Heun et al 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Labelling with BrdU and immunofluorescence detection Metabolic labelling of roots was carried out with 10 -4 M BrdU for 1 h, according to previous estimations (Cuadrado et al 1998). After treatment, the meristematic region of each root (0.5-1.5 mm from the root apex) was cut out, then fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS: 1.4 mM H 2 KPO 4 , 8 mM Na 2 HPO 4 , 2.7 mM KCl, 370 mM NaCl, pH 7.4) for 30 min at 4°C, and washed for 3·15 min in the same buffer.…”

Section: Flow-cytometry Analysismentioning

confidence: 99%

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Dynamics of replication foci and nuclear matrix during S phase in Allium cepa L. cells

Samaniego

Torre

Espina

2002

Planta

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The sequential organisation of replication foci during S phase in onion ( Allium cepa) and their relationship to the nuclear matrix were investigated. To discern their structural features and temporal firing sequence, immunodetection of 5-bromo-2'-deoxyuridine (BrdU) was carried out after in vivo feeding in synchronised cells released from a 14-h-long hydroxyurea block. Replication foci consisted of small replication granules, called replisomes, which clustered together. Analysis of synchronous binucleate cells that maintained in their two nuclei the specular symmetry of distribution of sister chromosomes in anaphase, showed that replication starts in small replication foci at the telomeric pole (pattern I), though the telomeres themselves formed large foci that were late-replicating. The rDNA replication foci (pattern II) also become replicated in early S phase. Replication of large foci, including the heterochromatin (IV), occurred in late S phase and finished at the centromeric nuclear pole (pattern V). Labelling of proliferating cell nuclear antigen (PCNA) in nuclear matrices, prepared from S-phase nuclei after extensive DNase digestion, demonstrated that replication foci were always stably anchored to the nuclear matrix. Thus, association with the nucleoskeleton is not exclusively mediated by the replicating or nascent DNA. The overlapping of patterns I, II and III in the nuclear matrix, in contrast to the results of BrdU localisation in nuclei, suggests that PCNA becomes associated with the nuclear matrix before the replication foci are operative, and remains bound during replication.

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Section: Nuclear Polarisation Of Replication Focisupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Flow-cytometry Analysismentioning

confidence: 99%

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Dynamics of replication foci and nuclear matrix during S phase in Allium cepa L. cells

Samaniego

Torre

Espina

2002

Planta

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The 5S rRNA genes in Alexandrium: their use as a FISH chromosomal marker in studies of the diversity, cell cycle and sexuality of dinoflagellates

et al. 2020

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Genome remodelling in three modern S. officinarumxS. spontaneum sugarcane cultivars

Cuadrado

Acevedo²,

Espina³

et al. 2004

Journal of Experimental Botany

108

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This study provides evidence that nuclear and chromosome remodelling has taken place in sugarcane, a vegetative crop with a complex genome derived from interspecific hybridizations between Saccharum officinarum and S. spontaneum. Detailed knowledge on the chromosomal compositions of the three clones analysed was acquired. (1) All hybrid cultivars were found to be aneuploid, affecting both parental genomes (having chromosomes in addition to full genomes), with chromosome numbers from 2n=102-106 in My5514 and up to 2n=113-117 in C236-51. (2) Comparative in situ hybridization showed that about 16% of these chromosomes are inherited from S. spontaneum and less than 5% are recombinant or translocated chromosomes containing sequences of both S. officinarum and S. spontaneum. (3) Differences between the observed DNA contents (estimated by flow cytometry) and those expected from the number of chromosomes, allowed the introgression of additional S. spontaneum or S. officinarum DNA pieces into the B42231 and C236-51 cultivars to be estimated. (4) Size heterogeneity between S. officinarum homologous chromosomes carrying the 18S-5.8S-25S and 5S ribosomal genes (identified by FISH with pTa71 and pTa794, respectively) confirms remodelling occurred by chromosomal interchange events, at least in these homologous chromosomes. (5) Simultaneous visualization of nucleoli and NORs showed that all 18S-5.8S-25S loci were potentially functional in the three clones, independent of their origin and size.

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Replication of 5 S ribosomal genes precedes the appearance of early nuclear replication complexes

Cited by 3 publications

References 28 publications

Dynamics of replication foci and nuclear matrix during S phase in Allium cepa L. cells

Dynamics of replication foci and nuclear matrix during S phase in Allium cepa L. cells

The 5S rRNA genes in Alexandrium: their use as a FISH chromosomal marker in studies of the diversity, cell cycle and sexuality of dinoflagellates

Genome remodelling in three modern S. officinarumxS. spontaneum sugarcane cultivars

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