Replication of mumps virus in mouse: transient replication in lung and potential of systemic infection

Abstract

A mumps virus strain, which replicated in mouse lung after aerosol inhalation, was obtained by selective replication of a wild strain in L929 cells and by further passaging in mice by intraperitoneal inoculation. All of infected mice survived and rechallenge of the survived mice with the same virus resulted in no virus growth in the lung. Treatment of infected mice with antiserum against interferon (IFN) or asialo GM1 delayed virus clearance from lung. Mice at 5 weeks of age were also sensitive to the virus as well as those at 1 week. When injected intravenously, the virus could grow not only in lung but also in salivary glands, heart and spleen. Furthermore, the virus replicated in liver, spleen, pancreas and testis after intraperitoneal inoculation. Antibody response of mice infected by aerosol inhalation was slower than that of intraperitoneally infected ones in either IgG or IgM production. These results indicated that the adapted virus replicated in mouse lung by a natural route of infection and had a potential to cause systemic infection in mouse.

Mumps Virus Decreases Testosterone Production and Gamma Interferon-Induced Protein 10 Secretion by Human Leydig Cells

“…Inflammation of the testis leads to hypofertility and testicular atrophy, which causes sterility that is usually transient but permanent in rare cases (8). Mumps virus replicates in the testes of mice (14), and a morphological study of the testes of patients infected with mumps virus showed degeneration of the seminiferous epithelium (4). The disruption of spermatogenesis may be a consequence of a drop in testosterone secretion by Leydig cells.…”

Establishing a small animal model for evaluating protective immunity against mumps virus

“…Based on previous work by others, mumps viruses do not productively replicate in mice nor cause any symptomatology [17, 18]. Similar problems with other RNA viruses, such as Junin virus, Dengue, and Ebola has been overcome by using mice lacking the IFN-α/β receptor (IFN-α/βR −/− ) [19–21].…”

Infection of Mice, Ferrets, and Rhesus Macaques with a Clinical Mumps Virus Isolate

“…Mice, as cost-efficient animals, are models for many human pathogens. However, mumps viruses are not known to replicate well or cause illness in adult mice (7,12,13). One mouse fibroblast cell-adapted mumps virus strain was reported to transiently replicate in the lungs of mice and might have circulated in the animal during infection (13).…”

“…However, mumps viruses are not known to replicate well or cause illness in adult mice (7,12,13). One mouse fibroblast cell-adapted mumps virus strain was reported to transiently replicate in the lungs of mice and might have circulated in the animal during infection (13). In most studies, mice were used to evaluate immune responses induced by different attenuated strains of mumps virus (12) or to evaluate memory B cell responses after MMR vaccination (14).…”