Replication of the Trichoplusia ni granulosis and nuclear polyhedrosis viruses in cell cultures

Granados, Robert R.; Derksen, Anja C.G.; Dwyer, Kathleen G.

doi:10.1016/0042-6822(86)90150-9

Cited by 63 publications

(41 citation statements)

References 14 publications

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“…Two insect cell lines, derived from Spodoptera frugiperda (Sf9) and Trichopulsia ni (Tn5 [BTI-Tn-5B1-4]), are frequently used (9,31). Tn5 cells have better secretion, which results in a higher productivity of recombinant proteins with the baculovirus expression system.…”

Section: Discussionmentioning

confidence: 99%

Latent Infection of a New Alphanodavirus in an Insect Cell Line

Scotti²,

Miyamura

et al. 2007

J Virol

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Insect BTI-TN-5B1-4 (Tn5) cells have been used extensively with recombinant baculoviruses to express foreign genes. When a recombinant baculovirus containing the hepatitis E virus capsid protein gene was used to infect Tn5 cells, unknown virus particles in addition to the anticipated hepatitis E virus-like particles were produced in the infected cells. The unknown virus particles were 35 nm in diameter and contained RNA that was highly homologous to full-length RNA1 (3,107 bp) and RNA2 (1,383 bp) genomic RNAs of flock house virus. Surprisingly, both RNAs seen in these induced nodavirus particles could be amplified from commercially available Tn5 cells without infection with or induction by a baculovirus. The nucleotide sequences from the purified nodavirus particles and the normal Tn5 cells were identical, demonstrating that the Tn5 cells themselves were latently infected with a nodavirus. However, the generation of nodavirus particles was significantly stimulated by infection with recombinant baculoviruses. Phylogenetic analysis suggested that this new nodavirus belongs to the genus Alphanodavirus in the family Nodaviridae.

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Section: Discussionmentioning

confidence: 99%

Latent Infection of a New Alphanodavirus in an Insect Cell Line

Scotti²,

Miyamura

et al. 2007

J Virol

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“…Slow growth rates have also been reported in the few other studies on GV-permissive cell lines. Four TnGV-permissive cell lines were shown to have a slower growth rate (1:5 split/week) than non-permissive lines (1 : 10 split/week) (Granados et al, 1986). Miltenburger et al (1984) selected two CpGV-permissive cell lines by weekly passage at split ratios of 1 : 4 and 1 : 2, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Difficulty has been experienced in selecting stable permissive cell lines for granulosis viruses (GVs). Only a few reports of in vitro GV infection have been published (Miltenburger et al, 1984;Naser et al, 1984;Naser, 1986;Granados et al, 1986;Dwyer et al, 1988) but the resulting cell lines had low susceptibility and/or were unstable, losing their virus-permissive character after serial passage. Thus, no cell line has so far been obtained with which it has been possible to carry out detailed studies on GV replication or GV molecular biology.…”

Section: Introductionmentioning

confidence: 99%

“…However, attempts to select virus-permissive cell lines from tissues in which GV replication is known to occur (fat body, intestine and epidermis) have also been unsuccessful (Miltenburger et al, 1984). Embryos have been shown to be the most successful source of GV-permissive cells and are derived from precursor cells of different origins (Miltenburger et al, 1984;Naser et al, 1984;Granados et al,, 1986;Dwyer et al, 1988). It appears that GVs are more fastidious in their host cell requirements since a much higher proportion of the primary cell lines that were tested for GV susceptibility were found to support NPV replication.…”

Section: Introductionmentioning

confidence: 99%

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Replication of Cydia pomonella granulosis virus in cell cultures

Winstanley¹,

Crook²

1993

Journal of General Virology

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Several primary cell lines that support the complete replication of Cydia pomonella granulosis virus have been established from one culture of C. pomonella embryonic cells. Virus passaged three times in cells and once in larvae showed no change in restriction enzyme fragment patterns. Stages in virus replication observed by electron microscopy resembled those from in vivo studies. Cell lines that were maintained at or below 21 °C retained susceptibility to virus over a period of 4 years whereas the same cell lines maintained at 27 °C gradually lost their susceptibility and eventually could not be infected at all.

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“…To date, only four SNPVs have been cultured in vitro: Helicoverpa (Heliothis) zea (HzSNPV) McIntosh & Ignoffo, 1981;Yamada et al, 1981), Orgyia ]eucostigma (OISNPV) (Sohi et al, 1984), Heliothis armigera (HaSNPV) (Zhu & Zhang, 1985) and Trichoplusia ni (TnSNPV) (Granados et aL, 1986). Of these four SNPVs, detailed biological characterization has been reported only for HzSNPV (Gettig, 1983;Gettig et al, 1987).…”

Section: Introductionmentioning

confidence: 99%