In vitro Characterization of a Trichoplusia ni Single Nucleocapsid Nuclear Polyhedrosis Virus

Influence of Baculovirus‐Host Cell Interactions on Complex N‐Linked Glycosylation of a Recombinant Human Protein

“…Accordingly, the cessation of host protein synthesis early in the AcMNPV replication cycle was reasoned to have a substantive effect on the structures and enzymatic activities associated with the endoplasmic reticulum and Golgi apparatus. Replication of TnSNPV and AcMNPV in Tn4h cells was similar with respect to the onset of viral DNA replication and production of budded virions (17). However, on the basis of a delayed cessation of host protein synthesis with TnSNPV infections as compared to AcMNPV infections, it was speculated that normal host processes, such as glycosylation, might proceed for longer periods post-infection with TnSNPV infections.…”

“…With AcMNPV infections, there is a cessation of host protein synthesis within 20 h post-infection. Following TnSNPV infection of Tn-4h cells, host protein synthesis continues for more than 36 h post-infection (17). In an attempt to retain the high expression levels achieved with the baculovirus expression vector system and perhaps retain co-and posttranslational processing capabilities for a longer time period post-infection, a recombinant TnSNPV expressing SEAP was constructed.…”

Evaluation of seven viral isolates as potential biocontrol agents against Pseudoplusia includens (Lepidoptera: Noctuidae) caterpillars

“…Therefore, the heterogeneity observed among the isolates here analyzed could be related with those bands present in submolar quantities on the gels. On the other hand, when our REN fragment gels were compared to other published REN fragment gels, since we have not yet PsinSNPV genome sequence data, the REN patterns of the PsinSNPV isolates were distinct from those other already known SNPV, such as Trichoplusia ni SNPV (Bilimoria, 1983;Davis and Wood, 1996;Erlandson et al, 2007), Buzura suppressaria SNPV (Liu et al, 1993), Orgyia antique SNPV (Richards et al, 1999), Orgya pseudotsugata SNPV (Leisy et al, 1986), Orgyia ericae SNPV (Yang et al, 2006), Helicoverpa (Heliothis) zea SNPV (Granados et al, 1981;McIntosh and Ignoffo, 1983), Heliothis armigera SNPV (Sudhakar and Mathavan, 1999), T. orichalcea SNPV (Cheng and Carner, 2000), A. honmai SNPV (Ishii et al, 2003;Takahashi et al, 2008). However, this comparison was not exhaustive and we still do not know whether the virus described here is a new virus or a variant of a previously described virus.…”

Genetic variation and virulence of Autographa californica multiple nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus isolates

“…LT 50 values ranged from approximately 69.3 hr (PlxyMNPV-CL3) to 100.3 hr (RoMNPV-R1) p. i. Prior studies in which the activity of a single isolate of AcMNPV was compared to the activity of a single isolate of TnSNPV yielded results in which either AcMNPV (Vail et al, 1971) or TnSNPV (Davis and Wood, 1996) killed larvae at lower doses. A study comparing a number of different AcMNPV and TnSNPV isolates revealed neither a consistent trend among the isolates nor a significant difference between AcMNPV and TnSNPV in bioassays against 2nd and 4th instar T. ni larvae (Erlandson et al, 2007).…”