Replication Process of the Parvovirus H-1 I. Kinetics in a Parasynchronous Cell System

Rhode, Solon L.

doi:10.1128/jvi.11.6.856-861.1973

Cited by 93 publications

(54 citation statements)

References 25 publications

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“…The full spectrum of replicative events -from adsorption of the parental virus to intranuclear accumulation of progeny virions -takes 6-8 h in a fully susceptible, replication-competent cell [38,39]. As mentioned earlier, 'competence' for replication of autonomous parvoviruses or dependoviruses thereby appears to be conferred to the cells by quite different mechanisms.…”

Section: Helper Functions In the Replication Of Autonomous Paruouirusesmentioning

confidence: 99%

“…In the case of autonomous parvoviruses, circumstantial observations in infected animals and experimental studies in cell cultures identified 'cycling' of cells, i.e., their active passage through the division cycle, as a major factor [38,40,41]. Using cell cultures in which the division cycles of individual cells had been synchronized by various treatments, the critical cellular function(s) then could be narrowed down to occur during duplication of the cellular genome (S-phase) [38,39,42]. Most investigators also agree that the critical function(s) become available in late S-phase.…”

Section: Helper Functions In the Replication Of Autonomous Paruouirusesmentioning

confidence: 99%

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Parvoviruses: agents of distinct pathogenic and molecular potential

Siegl

Tratschin

1987

FEMS Microbiology Letters

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Parvoviruses are small, spherical and extremely stable particles with a genome of linear single‐stranded DNA. They are widespread in nature and thus have been in virtually every animal species and in man. Many parvoviruses were initially isolated by chance and could not be associated with clinical disease. Today, however, a considerable number of these agents are known to cause a broad spectrum of syndromes including fetal death, malformations, dwarfism, cerebellar hypolasia and ataxia, enteritis, panleukopenia, aplastic anemia, and immunologic disorders. Biological, pathological, and molecular studies revealed that all these syndromes are explainable on the basis of two predominant requirements for productive cytolytic parvovirus replication: the availability of cellular helper function(s) provided exclusively by mitotically active cells in the phase of cellular DNA replication, and by apparently specific, yet so far undefined cellular functions

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Section: Helper Functions In the Replication Of Autonomous Paruouirusesmentioning

confidence: 99%

Section: Helper Functions In the Replication Of Autonomous Paruouirusesmentioning

confidence: 99%

Parvoviruses: agents of distinct pathogenic and molecular potential

Siegl

Tratschin

1987

FEMS Microbiology Letters

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“…Minute virus of mice (MVM), a member of the helperindependent parvovirus group (12), contains a linear singlestranded DNA molecule of 5,081 nucleotides (3) that replicates in the S-phase nucleus of susceptible rodent cells grown in tissue culture (10,12,15,17). The termini of MVM single-stranded (SS) DNA are folded in a base-paired hairpin configuration (1,2,4,5) and play an essential role in viral DNA replication.…”

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confidence: 99%

Characterization of a metastable, partially replicated dimeric intermediate of minute virus of mice

Faust¹,

Gloor²

1984

J Virol

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Intracellular, replicative-form DNA of minute virus of mice was characterized by agarose gel electrophoresis, velocity sedimentation, electron microscopy, restriction endonuclease digestion, and sensitivity to the single-stranded nuclease S1. This analysis demonstrated the presence in murine cells infected with minute virus of mice of a 10.0-kilobase pair dimer replicative form, a 5-kilobase pair monomer replicative form, as well as a 5-kilobase viral single-stranded DNA species. Two additional viral DNA species that migrated in 0.5% agarose gels with apparent sizes of 8.0 and 5.5 kilobase pairs were also observed. Further investigation indicated that the 8.0-kilobase pair DNA represents a novel class of metastable, partially replicated, dimeric intermediates. This finding has important implications for the mechanism of parvovirus DNA replication.

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“…The absence of rMVMpmediated transgene expression in these cells is therefore not surprising, on account of the well-known S-phase dependence of the parvoviral life cycle. [26][27][28][29] In the other, proliferating normal cells, the low level of transgene expression clearly indicates that rMVMp selectivity also depends on other cell properties, such as the differentiation state of the host cells (and especially, on whether they have undergone neoplastic transformation). This is precisely what makes such vectors interesting for tumor cell-targeted gene therapy.…”

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confidence: 99%

True

2000

Gene Ther

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A recombinant MVMp of the fibrotropic strain of minute virus of mice (MVMp) expressing the chloramphenicol acetyltransferase reporter gene was used to infect a series of biologically relevant cultured cells, normal or tumor-derived, including normal melanocytes versus melanoma cells, normal mammary epithelial cells versus breast adenocarcinoma cells, and normal neurons or astrocytes versus glioma cells. As a reference cell system we used normal human fibroblasts versus the SV40-transformed fibroblast cell line NB324K. After infection, we observed good expression of the reporter gene in the different tumor cell types, but only poor expression if any in the corresponding normal cells. We also constructed a recombinant MVMp expressing the green fluorescent protein reporter gene and assessed by flow cytometry the efficiency of gene transduction into the different target cells. At a multiplicity of infection of 30, we observed substantial transduction of the gene into most of the tumor cell types tested, but only marginal transduction into normal cells under the same experimental conditions. Finally, we demonstrated that a recombinant MVMp expressing the herpes simplex virus thymidine kinase gene can, in vitro, cause efficient killing of most tumor cell types in the presence of ganciclovir, whilst affecting normal proliferating cells only marginally if at all. However, in the same experimental condition, breast tumor cells appeared to be resistant to GCV-mediated cytotoxicity, possibly because these cells are not susceptible to the bystander effect. Our data suggest that MVMp-based vectors could prove useful as selective vehicles for anticancer gene therapy, particularly for in vivo delivery of cytotoxic effector genes into tumor cells.

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Replication Process of the Parvovirus H-1 I. Kinetics in a Parasynchronous Cell System

Cited by 93 publications

References 25 publications

Parvoviruses: agents of distinct pathogenic and molecular potential

Parvoviruses: agents of distinct pathogenic and molecular potential

Characterization of a metastable, partially replicated dimeric intermediate of minute virus of mice

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