Report: <i>In vitro</i> antimicrobial susceptibility of <i>Propionibacterium acnes</i> isolated from acne patients in northern Mexico

González, R. de la Cruz; Welsh, Oliverio; Ocampo, Jorge; Hinojosa-Robles, Rosa Maria; Vera-Cabrera, Lucio; Delaney, Mary L.; Gómez, Minerva

doi:10.1111/j.1365-4632.2010.04506.x

Cited by 45 publications

(53 citation statements)

References 28 publications

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“…Several other reports about increasing AZM resistance have been published recently (1,12,26). However, we did not find any evidence of acquisition of resistance after exposure to sub-MICs of AZM or ERY (Table 3).…”

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confidence: 53%

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Maezono

Noiri

Asahi

et al. 2011

Antimicrob Agents Chemother

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Antibiotic resistance of biofilm-grown bacteria contributes to chronic infections, such as marginal and periapical periodontitis, which are strongly associated with Porphyromonas gingivalis. Concurrent azithromycin (AZM) administration and mechanical debridement improve the clinical parameters of periodontal tissue in situ. We examined the in vitro efficacy of AZM against P. gingivalis biofilms. The susceptibilities of adherent P. gingivalis strains 381, HW24D1, 6/26, and W83 to AZM, erythromycin (ERY), ampicillin (AMP), ofloxacin (OFX), and gentamicin (GEN) were investigated using a static model. The optical densities of adherent P. gingivalis cells were significantly decreased by using AZM and ERY at sub-MIC levels compared with those of the controls in all the strains tested, except for the effect of ERY on strain W83. AMP and OFX inhibited P. gingivalis adherent cells at levels over their MICs, and GEN showed no inhibition in the static model. The effects of AZM and ERY against biofilm cells were investigated using a flow cell model. The ATP levels of P. gingivalis biofilms were significantly decreased by AZM at concentrations below the sub-MICs; however, ERY was not effective for inhibition of P. gingivalis biofilm cells at their sub-MICs. Furthermore, decreased density of P. gingivalis biofilms was observed three-dimensionally with sub-MIC AZM, using confocal laser scanning microscopy. These findings suggest that AZM is effective against P. gingivalis biofilms at sub-MIC levels and could have future clinical application for oral biofilm infections, such as chronic marginal and periapical periodontitis.

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confidence: 53%

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Maezono

Noiri

Asahi

et al. 2011

Antimicrob Agents Chemother

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“…There is widespread resistance in P. acnes due to overuse of topical and systemic antibiotics for treatment of acne vulgaris [3,[8][9][10][11][12][13][14][15][16], as shown in Table 4. Various studies have used different interpretative criteria to estimate the resistance among P. acne strains to different anti-acne drugs.…”

Section: Discussionmentioning

confidence: 99%

In vitro antimicrobial susceptibility patterns of Propionibacterium acnes isolated from patients with acne vulgaris

Biswal

Gaind

Kumar

et al. 2016

J Infect Dev Ctries

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Introduction: Propionibacterium acnes has been implicated in the development of acne vulgaris. Rampant use of topical and systemic antibiotics for acne vulgaris has led to resistance due to selective pressure. This study aimed to determine antibiotic resistance of P. acnes. Methodology: A total of 102 samples were collected from acne lesions and cultured onto sheep's blood agar and brain-heart infusion agar supplemented with 5 g/L glucose and 2 mg/L furazolidone) (BHIg) under aerobic and anaerobic conditions. Species identification was done by conventional methods and the VITEK2 Compact system. The isolates were tested for penicillin, erythromycin, clindamycin, ciprofloxacin, nadifloxacin, and tetracycline by E-test, and minimum inhibitory concentration (MIC) of minocycline was determined by agar dilution on BHIg. MIC results were interpreted as per EUCAST (European Committee on Antimicrobial Susceptibility Testing) and CLSI (Clinical Laboratory Standards Institute) guidelines. Results: P. acnes was the most common anaerobe (66%) isolated. Resistance rates using EUCAST and CLSI breakpoints were 10.6% and 6.1%, 7.6% and 0%, 7.8% and 0% for erythromycin, clindamycin, and minocycline, respectively. Tetracycline resistance was observed in 9.2% isolates irrespective of the interpretative criteria used. MIC50 and MIC90 values for nadifloxacin (0.25 and 1 µg/mL) were found to be twofold lower than those for ciprofloxacin (0.5 and 1 µg/mL). Similarly, MIC50 and MIC90 values for minocycline (0.125 and 0.5 µg/mL) were also two-to threefold lower than those for tetracycline (0.38 and 1 µg/mL). Conclusions: To the best of our knowledge, this is the first study focusing on P. acnes resistance from India.

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“…In the present study, which is the first to report data on the antimicrobial susceptibility of P. acnes in Colombia, 40% of the isolates were resistant to at least one of the antibiotics tested. In the few other studies conducted in Latin America, the prevalence of isolates resistant to at least one antibiotic was not specified in Costa Rica 23 and was specified as 8% in Chile 24 and 76% in Mexico 25 …”

Section: Discussionmentioning

confidence: 99%

“…In a 10‐year study conducted in the UK between 1991 and 2000, erythromycin resistance was the most commonly found resistance in all years, reaching a peak of 58% in 1997 12 . High prevalences of erythromycin resistance relative to overall resistance were observed in Costa Rica (19%), 23 Chile (4%), 24 and Mexico (46%) 25 …”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of Propionibacterium acnes isolates from acne patients in Colombia

et al. 2013

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Report: In vitro antimicrobial susceptibility of Propionibacterium acnes isolated from acne patients in northern Mexico

Cited by 45 publications

References 28 publications

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

In vitro antimicrobial susceptibility patterns of Propionibacterium acnes isolated from patients with acne vulgaris

Antimicrobial susceptibility of Propionibacterium acnes isolates from acne patients in Colombia

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