Report of SARS-CoV-2 B1.1.7 Lineage in Morocco

Ouadghiri, Mouna; Aanniz, Tarik; Essabbar, Abdelomunim; Seffar, Myriam; Kabbaj, Hakima; Amin, Ghizlane El; Zouaki, Amal; Amzazi, Saaïd; Belyamani, Lahcen; Ibrahimi, Azeddine

doi:10.1128/mra.00240-21

Cited by 6 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the detection of the RdRp gene varies depending on the thermal cycler: with the QS5, the target was detected in all samples, while with the ABI and Exicycler, the target was not detected in 1/ 52 and 7/52 samples, respectively. Moreover, the S target of the MAScIR SARS-CoV-2 M 2.0 kit is not afected by the mutation of the Alpha, Delta, or Omicron variant since the Ct of the RdRp and S genes were identical, which was confrmed by the sequencing of our samples (Tables 5 and 6) [15,16]. Our entire dataset has been published on the Mendeley Data Repository [17].…”

Section: Discussionmentioning

confidence: 67%

Evaluation of the MAScIR SARS-CoV-2 M Kit 2.0 on the SARS-CoV-2 Infection

Zouaki

Kabbaj

Amin

et al. 2023

Advances in Virology

Self Cite

View full text Add to dashboard Cite

SARS-CoV-2 is a major public health problem worldwide. Since its emergence, several diagnostic kits have been developed to ensure rapid patient management. The aim of our study is to check the performance of the new Moroccan SARS-CoV-2 detection kit: MAScIR SARS-CoV-2 M 2.0. The following parameters were studied: repeatability, reproducibility, analytical specificity, analytical sensitivity, and comparison with the GeneFinder™ COVID-19 Plus RealAmp Kit. In addition, an external quality evaluation comprising five specimens was carried out as part of an international program for the external quality evaluation of sublaboratories of the WHO and the Laboratory Office of the National Institute of Hygiene of Morocco. The results of all parameters studied showed an analytical performance that complied with the requirements of the method verification/validation protocol adopted by the Central Laboratory of Virology and met the recommendations of COFRAC (French Accreditation Committee). During the current study, the sequencing of some randomly selected positive samples was performed, among which the carriers of the Alpha variant, the Delta variant, and the Omicron variant were detected. These results allowed us to deduce that this kit was valid for detecting these three variants.

show abstract

Section: Discussionmentioning

confidence: 67%

Evaluation of the MAScIR SARS-CoV-2 M Kit 2.0 on the SARS-CoV-2 Infection

Zouaki

Kabbaj

Amin

et al. 2023

Advances in Virology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Clade 19B continued this trend, demonstrating incremental genetic shifts that influenced the virus’s transmissibility and host interactions. Subsequently, after the first year, the Alpha variant (Lineage B.1.1.7) emerged in the United Kingdom, demonstrating increased transmissibility and rapid global spread [ 8 , 9 ]. Simultaneously, the Beta variant (Lineage B.1.351) was identified in South Africa, characterized by mutations that influenced virus-host interactions and immune evasion, marking significant shifts in the genomic landscape.…”

Section: Introductionmentioning

confidence: 99%

Temporal Dynamics and Genomic Landscape of SARS-CoV-2 After Four Years of Evolution

Essabbar,

El Mazouri,

Boumajdi

et al. 2024

Cureus

Self Cite

View full text Add to dashboard Cite

Introduction Since its emergence, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has undergone extensive genomic evolution, impacting public health policies, diagnosis, medication, and vaccine development. This study leverages advanced bioinformatics to assess the virus’s temporal and regional genomic evolution from December 2019 to October 2023. Methods Our analysis incorporates 16,575 complete SARS-CoV-2 sequences collected from 214 countries. These samples were comparatively analyzed, with a detailed characterization of nucleic mutations, lineages, distribution, and evolutionary patterns during each year, using the Wuhan-Hu-1 strain as the reference. Results Our analysis has identified a total of 21,580 mutations that we classified into transient mutations, which diminished over time, and persistent mutations with steadily increasing frequencies. This mutation landscape led to a notable surge in the evolutionary rate, rising from 13 mutations per sample in 2020 to 96 by 2023, with minor geographic variations. The phylogenetic analysis unveiled three distinct evolutionary branches, each representing unique viral evolution pathways. These lineages exhibited a tendency for a reduced duration of dominance with a shortening prevalence period over time, as dominant strains were consistently replaced by more fit variants. Notably, the emergence of the Alpha and Delta variants in 2021 was followed by the subsequent dominance of Omicron clade variants that have branched into several recombinant variants in 2022, marking a significant shift in the viral landscape. Conclusion This study sheds light on the dynamic nature of SARS-CoV-2 evolution, emphasizing the importance of continuous and vigilant genomic surveillance. The dominance of recombinant lineages, coupled with the disappearance of local variants, underscores the virus’s adaptability.

show abstract

“…Since the beginning of the COVID-19 pandemic, genomic analysis of Moroccan samples has revealed the existence of variants of concern (VOCs). The emergence of these variants has been accompanied by waves of infection; the first wave was associated with the dominance of the alpha variant (B.1.1.7) in early February 2021 [6], and the second wave was associated with the appearance of the delta variant (B.1.617.2) in June 2021.…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and genomic analysis of SARS-CoV-2 delta variant in Morocco

Mazouri

Bendani²,

Boumajdi³

et al. 2022

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

Introduction: Since the COVID-19 pandemic began in December 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has continuously evolved with many variants of concern emerging across the world. Methodology: In order to monitor the evolution of these variants in Morocco, we analyzed a total of 2130 genomes of the delta variant circulating around the world. We also included 164 Moroccan delta variant sequences in our analysis. Results: Our findings suggest at least four introductions from multiple international sources and a rise of a dominant delta sub-lineage AY.33 in Morocco. Moreover, we report three mutations in the N-terminal domain of the S protein specific to the Moroccan AY.33 isolates, T29A, T250I and T299I. The effect of these mutations on the secondary structure and the dynamic behavior of the S protein N-terminal domain was further determined. Conclusions: We conclude that these mutations might have functional consequences on the S protein of SARS-CoV-2.

show abstract

Report of SARS-CoV-2 B1.1.7 Lineage in Morocco

Cited by 6 publications

References 11 publications

Evaluation of the MAScIR SARS-CoV-2 M Kit 2.0 on the SARS-CoV-2 Infection

Evaluation of the MAScIR SARS-CoV-2 M Kit 2.0 on the SARS-CoV-2 Infection

Temporal Dynamics and Genomic Landscape of SARS-CoV-2 After Four Years of Evolution

Phylogeography and genomic analysis of SARS-CoV-2 delta variant in Morocco

Contact Info

Product

Resources

About