Report of the committee on the genetic constitution of chromosomes 1 and 2

Povey, S.; Morton, N.E.; Sherman, S.L.

doi:10.1159/000132170

Cited by 40 publications

(14 citation statements)

References 49 publications

(75 reference statements)

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“…This result places the MCADase locus just distal to the loci NRAS, PGMI, NGFB, TSHB, and MSKI and immediately proximal to FUCA], UROD, AK2, UMPK, BLYM, and MYCL (35). Thus, the assigned position of the human MCADase gene makes it a reference point for a detailed linkage map of chromosome 1.…”

Section: Discussionmentioning

confidence: 88%

Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.

Matsubara¹,

Kraus²,

Yang‐Feng³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Rat liver mRNA encoding the precursor of medium-chain acyl-CoA dehydrogenase was purified to near homogeneity by polysome immunoadsorption using a polyclonal, monospecific antibody. A single-stranded, 32P-labeled cDNA probe was synthesized using the enriched mRNA as template and was used to screen directly 15,000 colonies from a total rat liver cDNA library constructed in pBR322. One clone [600 base pairs (bp)] was positively identified by hybrid-selected translation combined with mitochondrial processing of translated products. Using the isolated rat cDNA as probe, 43,000 colonies from a human liver cDNA library were screened. Three overlapping clones (1100 bp, 500 bp, and 400 bp) were isolated and positively identified by hybrid-selected translation. The largest human cDNA clone was subcloned into the transcription vector pGEM-2, which contains a bacteriophage T7 RNA polymerase promoter. In vitro transcription of this recombinant, followed by in vitro translation, showed that the cDNA clone coded for approximately 80% of the medium-chain acyl-CoA dehydrogenase protein. The sizes of rat and human mRNAs encoding the precursor of medium-chain acyl-CoA dehydrogenase were 2.2 and 2.4 kilobases long, respectively, as determined by blot hybridization analysis of electrophoretically fractionated poly(A)+ RNA. Southern blot analysis of DNAs from human-rodent somatic cell hybrids with an isolated human cDNA assigned the gene coding for this enzyme to the short arm of chromosome 1, band p31. The chromosomal assignment was confirmed by in situ hybridization of the probe to human metaphase cells. Direct screening of cDNA libraries using a highly enriched mRNA to generate a probe, as demonstrated in this study, may provide the most rapid and convenient approach to cDNA cloning of low-abundance mRNAs.

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Section: Discussionmentioning

confidence: 88%

Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.

Matsubara¹,

Kraus²,

Yang‐Feng³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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“…sources could not exclude all the doubts on the JK, CO and KEL assignments to chromosome 2 [Lewis et al, 1982;Povey et al, 1985]. The recent discovery of Geitvik et al [1986] on close linkage between the J K locus and the genomic probe L 2.7 (z = 8.46, 0 = 0.05) must therefore be consid ered of great importance for the definite JK gene localisation.…”

mentioning

confidence: 97%

“…Linkage analyses of locus pairs involving IGK, JK, CO, KEL and AHCY resulted in altogether negative lod scores, thereby dwindling the reported linkage rela tions.The Colton, Kidd and Kell blood group loci (CO, JK, KEL) have to date eluded secure chromosomal assignment [Lewis et al;1982;Povey et al, 1985], A number of reports favoured, however, their localisation to chromosome 2, be cause highly positive lod scores were found between the IGK locus (located on 2p 12) and the JK locus [z = 3.14,0 = 0.31; Sherman and Simpson, 1985) on the one hand and moderately positive JK:CO [z = 2.60; 0 = 0.32; Sherman and Simpson, 1985] and CO: K EL lod scores [z = 1.01,0 = 0.30; Lewis et al, 1982] on the other. …”

mentioning

confidence: 99%

“…In the first 6 locus pairs, also the available lod scores of Human Gene Mapping 8 (HGM 8) [Povey et al, 1985] or respectively those of Lewis et al [1982] were given for comparison with the last line (T, total) being the sum of lines three (J)and four. sylhomocysteine hydrolase, EC 3.3.1.1), especially because Bissbort et al [1983] have reported weakly positive KEL: AHCY lod scores (z = 0.42,0 = 0.05).…”

mentioning

confidence: 99%

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Linkage Relations of JK, CO, KEL and IGK with Each Other and with AHCY

Bender

Bissbort²,

Crone³

et al. 1988

Hum Hered

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“…A clone bank was prepared from the distal region of the short arm of human chromosome 2. This region comprises approximately 1.5% of the human genome and has few DNA markers (22) and the additional advantage of being easy to identify under phase-contrast microscopy without staining. The region includes the known oncogene N-myc, which is amplified in neuroblastomas, and may also contain the loci defining certain cases of autosomal dominant aniridia, coloboma (1), and cancer family syndrome (22 repeated until DNA had been collected from 106 chromosomes ( Fig.…”

mentioning

confidence: 99%

Microdissection of and microcloning from the short arm of human chromosome 2.

Bates¹,

Wainwright²,

Williamson³

et al. 1986

Mol. Cell. Biol.

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A bank of cloned DNA sequences from the distal half of the short arm of human chromosome 2 was generated by using microdissection and microcloning techniques. DNA was purified from 106 chromosomal fragments, manually dissected from peripheral lymphocytes in metaphase, and cloned into the EcoRI site of XgtlO. A total of 257 putative recombinants were recovered, of which 41% were found to contain human inserts. The mean insert size was 380 base pairs (median size, 83 base pairs), and fewer than 10% of the clones contained highly repetitive sequences. All single-copy sequences examined were shown to map to the short arm of chromosome 2 by using hybrid panels. This technique provides a rapid method of isolating probes specific to a human subchromosomal region to generate linked markers to genetic diseases for which the chromosomal location is known.

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Report of the committee on the genetic constitution of chromosomes 1 and 2

Cited by 40 publications

References 49 publications

Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.

Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.

Linkage Relations of JK, CO, KEL and IGK with Each Other and with AHCY

Microdissection of and microcloning from the short arm of human chromosome 2.

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