Report on the ocurrence of Angiostrongylus costaricensis in southern Brazil, in a new intermediate host from the genus Sarasinula (Veronicellidae, Gastropoda)

Laitano, Antonio Carlo; Genro, Júlia Pasquali; Fontoura, Ricardo; Branco, Susana S. L.; Maurer, Rafael Lucyk; Graeff-Teixeira, Carlos; Milanez, José Maria; Chiaradia, Luís Antonio; Thomé, José Willibaldo

doi:10.1590/s0037-86822001000100015

Cited by 26 publications

(15 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the extent to which these types of mollusc are accessible to definitive hosts may vary. A broad intermediate host range has also been reported for other Angiostrongylus species, such as A. cantonensis [ 42 , 43 ] and A. costaricensis [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 94%

Distribution of Angiostrongylus vasorum and its gastropod intermediate hosts along the rural–urban gradient in two cities in the United Kingdom, using real time PCR

et al. 2016

View full text Add to dashboard Cite

BackgroundAngiostrongylus vasorum is a highly pathogenic metastrongylid nematode affecting dogs, which uses gastropod molluscs as intermediate hosts. The geographical distribution of the parasite appears to be heterogeneous or patchy and understanding of the factors underlying this heterogeneity is limited. In this study, we compared the species of gastropod present and the prevalence of A. vasorum along a rural–urban gradient in two cities in the south-west United Kingdom.MethodsThe study was conducted in Swansea in south Wales (a known endemic hotspot for A. vasorum) and Bristol in south-west England (where reported cases are rare). In each location, slugs were sampled from nine sites across three broad habitat types (urban, suburban and rural). A total of 180 slugs were collected in Swansea in autumn 2012 and 338 in Bristol in summer 2014. A 10 mg sample of foot tissue was tested for the presence of A. vasorum by amplification of the second internal transcribed spacer (ITS-2) using a previously validated real-time PCR assay.ResultsThere was a significant difference in the prevalence of A. vasorum in slugs between cities: 29.4 % in Swansea and 0.3 % in Bristol. In Swansea, prevalence was higher in suburban than in rural and urban areas. Comparing the sampled slug fauna, Arion rufus was found in greater numbers in Swansea than Bristol, and was commonly infected (prevalence 41 %). This, alongside the timing of slug collections in summer rather than autumn, could explain low infection prevalence in the Bristol sample. In the absence of Ar. rufus as a preferred host for A. vasorum, Ar. flagellus and Limacus maculatus appear to act as versatile hosts that are present in suburban and urban areas in Swansea (prevalence in Ar. flagellus 33 %; in L. maculatus 44 %) and in Bristol (prevalence in Ar. flagellus 0.9 %). These slug species might provide A. vasorum with an alternative vehicle to reach the final host, when the main host Ar. rufus is scarce or absent.ConclusionWe conclude that the composition of the slug fauna varies spatially, and that this could help explain patchiness in the prevalence of A. vasorum. A suburban peak was found in the prevalence of infection in intermediate hosts, perhaps explained by a higher density of competent intermediate and/or definitive hosts.

show abstract

Section: Discussionmentioning

confidence: 94%

Distribution of Angiostrongylus vasorum and its gastropod intermediate hosts along the rural–urban gradient in two cities in the United Kingdom, using real time PCR

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Mollusks from the Veronicellidae family are the main intermediate hosts in endemic areas in Costa Rica (Morera and Ash 1971), Ecuador (Morera et al 1983), Honduras (Kaminsky et al 1987), Nicaragua (Duarte et al 1992), and Brazil (Graeff-Teixeira et al 1989;Rambo et al 1997;Bonetti and Graeff-Teixeira 1998;Laitano et al 2001). However, several species belonging to other Pulmonata families were also found naturally infected (Graeff-Teixeira et al 1993;Rambo et al 1997), besides the families that were shown as potential hosts in experimental trials (Ubelaker et al 1980;Lima et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Montresor

Vidigal

Mendonça³

et al. 2008

Parasitol Res

View full text Add to dashboard Cite

Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with stage 1 larvae. Obtained stage 3 larvae were orally inoculated in mice, and after 30 days, adult worms and stage 1 larvae were recovered, demonstrating Omalonyx susceptibility and suitability to infection. To define the parasite migration routes, specimens of Omalonyx with 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 2 days, 5 days, 10 days, 12 days, 15 days, 20 days, 21 days, 25 days, 28 days, and 30 days of infection were fixed and serially sectioned. Histological sections were stained with hematoxylin-eosin. The results were compared to those described in S. marginata. Oral and cutaneous infections were noted. After the penetration, larvae were retained, mainly in the fibromuscular tissue, by hemocytes, or they spread to the whole organism through the circulation, following the anatomical structure of the vasculature. The perilarval hemocyte reaction in Omalonyx was more intense until stage 2 larva instar, decreasing in the presence of stage 3 larvae. Differences in some aspects of hemocyte reaction between S. marginata and Omalonyx exemplify interspecific peculiarities in snail response to the same parasite.

show abstract

“…Several other terrestrial molluscs have also been found to be naturally infected with this nematode in Brazil 2 , 3 , 8 , 19 and in other countries 9 , 13 , 14 . Among these species are S. linguaeformis and S. octona 2 , 3 , 8 , 9 , which, however, were not found to be infected in the present study. A possible reason for this is the lower abundance of these species in the sampled localities.…”

Section: Discussionmentioning

confidence: 99%

First records of molluscs naturally infected with Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) in Sergipe State, Northeastern Brazil, including new global records of natural intermediate hosts

Ramos-de-Souza

Thiengo

Fernandez

et al. 2018

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

Human neural angiostrongyliasis is an emerging infectious disease caused by nematode Angiostrongylus cantonensis. The present study investigated the presence of Angiostrongylus spp. in terrestrial molluscs collected from the following areas in the Metropolitan Region of Aracaju, Sergipe State, Brazil: Barra dos Coqueiros, Nossa Senhora do Socorro, Sao Cristovao and Aracaju. In total, 703 specimens representing 13 mollusc species were screened for Angiostrongylus spp. Larvae of Angiostrongylus spp. were found in three species. Larvae recovered from Achatina fulica were used for experimental infection in Wistar rats (Rattus norvegicus). For specific identification of nematodes, the mitochondrial cytochrome c oxidase subunit I (COI) was sequenced from both larvae and adults recovered from molluscs and rats, respectively. Infection with A. cantonensis was detected in all municipalities and in the following three host species: Bulimulus tenuissimus, Cyclodontina fasciata (Barra dos Coqueiros), and A. fulica (Aracaju, Nossa Senhora do Socorro and Sao Cristovao). Co-infections were also found with Caenorhabditis sp. and Strongyluris sp. larvae. This is the first study of the helminth fauna associated with the terrestrial malacofauna in Sergipe State, and confirms that these three snail species are involved in the transmission of A. cantonensis in the state. In addition, B. tenuissimus and C. fasciata are newly reported natural hosts of the parasite.

show abstract

Report on the ocurrence of Angiostrongylus costaricensis in southern Brazil, in a new intermediate host from the genus Sarasinula (Veronicellidae, Gastropoda)

Cited by 26 publications

References 4 publications

Distribution of Angiostrongylus vasorum and its gastropod intermediate hosts along the rural–urban gradient in two cities in the United Kingdom, using real time PCR

Distribution of Angiostrongylus vasorum and its gastropod intermediate hosts along the rural–urban gradient in two cities in the United Kingdom, using real time PCR

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

First records of molluscs naturally infected with Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) in Sergipe State, Northeastern Brazil, including new global records of natural intermediate hosts

Contact Info

Product

Resources

About