In 2020, due to the COVID-19 pandemic, animal physiology practical classes at The University of Queensland, were conducted fully online using Lt, a cloud-based platform, ensuring that students were able to achieve the intended course outcomes. However, in 2021, practical classes had to be redesigned to cater for both on-campus and online deliveries and this project investigated the continued use of Lt in animal physiology practical classes focusing on student satisfaction and performance for both on-campus and online students. Student satisfaction with the redesigned practicals were high across all constructs - overall structure, pre-lab materials and incorporation of group work (on-campus students) while student performance outcomes showed an improved performance compared to before the use of Lt and showed no significant differences in the performance of the on-campus versus online cohorts. The findings gave confidence in using Lt as a foundation for delivering animal science practicals equitably for both cohorts.