Repression of Dpp targets in the<i>Drosophila</i>wing by Brinker

Winter, Stephanie E.; Campbell, Gerard

doi:10.1242/dev.01538

Cited by 40 publications

(33 citation statements)

References 43 publications

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“…Either Atro has some activity in the absence of Sbb or C15 and En can use mechanisms other than recruitment of Gro and Atro to repress transcription. Many transcription factors have been shown to have the ability to repress by several mechanisms; for example, although Brk recruits both CtBP and Gro, it can repress some genes in the absence of both, using additional repression domains (Winter and Campbell 2004).…”

Section: Discussionmentioning

confidence: 99%

“…In reality, it is often more difficult to understand exactly why a particular transcription factor recruits more than one corepressor. For Brk, CtBP and Gro are largely redundant (Hasson et al 2001;Winter and Campbell 2004), while for Hairy there is evidence that it may selectively recruit each corepressor for repression of specific targets (Bianchi-Frias et al 2004).…”

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confidence: 99%

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Genetic Interactions Among scribbler, Atrophin and groucho in Drosophila Uncover Links in Transcriptional Repression

Wehn

Campbell

2006

Genetics

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In eukaryotes, the ability of DNA-binding proteins to act as transcriptional repressors often requires that they recruit accessory proteins, known as corepressors, which provide the activity responsible for silencing transcription. Several of these factors have been identified, including the Groucho (Gro) and Atrophin (Atro) proteins in Drosophila. Here we demonstrate strong genetic interactions between gro and Atro and also with mutations in a third gene, scribbler (sbb), which encodes a nuclear protein of unknown function. We show that mutations in Atro and Sbb have similar phenotypes, including upregulation of the same genes in imaginal discs, which suggests that Sbb cooperates with Atro to provide repressive activity. Comparison of gro and Atro/sbb mutant phenotypes suggests that they do not function together, but instead that they may interact with the same transcription factors, including Engrailed and C15, to provide these proteins with maximal repressive activity.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Genetic Interactions Among scribbler, Atrophin and groucho in Drosophila Uncover Links in Transcriptional Repression

Wehn

Campbell

2006

Genetics

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“…Generating Brk-VP16 construct DNA contains the first 173 aa of the Brk repressor, includes the DNA-binding domain (DBD) site, nuclear localisation sequence (NLS), but not the repression domain (construct A2 from (Winter and Campbell, 2004)) cloned into pUAST vector that contains VP16 activation domain (a gift from Dr Adi ZalsbergTechnion). 50 mg of Qiagen-purified DNA sent to Genetic Services Inc. (GSI) where they injected the DNA into mutant embryos lacking the gene W (for red eyes).…”

Section: Immunohistochemistry and Imagingmentioning

confidence: 99%

Inverse regulation of target genes at the brink of the BMP morphogen activity gradient

Ziv

Finkelstein

Suissa

et al. 2012

Journal of Cell Science

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Summary BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk). This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak. Here, we show that in contrast to the reciprocal repressor gradient mechanism, where Brk represses BMP targets in medial regions, target expression in lateral regions is downregulated by BMP signalling and activated by Brk. Brk induces lateral expression indirectly, apparently through repression of a negative regulator. Our findings provide a model explaining how the expression of an established BMP target is differentially and inversely regulated along the anterior-posterior axis of the wing disc.

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“…The mechanism of repression by Brinker does not rely on competition with Mad-Medea overlapping sites, but on the existence of adjacent binding sites for Brinker and Mad/Med (Barrio and de Celis, 2004). Additional factors such as the T-box transcription factor Optomotor blind, the trithorax protein Ash2, the activator complex Vestigial/Scalloped and the repressor Groucho are also involved in the regulation of sal in the wing blade (Guss et al, 2001;del Alamo Rodriguez et al, 2004;Angulo et al, 2004;Winter and Campbell, 2004;Hasson et al, 2005). The enhancer regulating salr expression in the wing blade has not yet been identified.…”

Section: Regulation Of Sall Gene Expressionmentioning

confidence: 99%

Regulation and function of Spalt proteins during animal development

Celis¹,

Barrio²

2009

Int. J. Dev. Biol.

124

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The genes of the spalt (sal) family play fundamental roles during animal development. The two members of this family in Drosophila, spalt (sal) and spalt-related (salr) encode Znfinger transcription factors that link the Decapentaplegic (Dpp)/BMP signalling pathway to the patterning of the wing. They are regulated by the Dpp pathway in the wing disc, and they were shown to mediate some of the morphogenetic activities of the Dpp/BMP4 secreted ligand. The sal genes were initially found by virtue of mutations that produce homeotic transformations in the head and tail of the Drosophila embryo. Since then, a number of other requirements have been associated to these genes in Drosophila, including morphogenesis of the respiratory system, cell fate specification of sensory organs and the differentiation of several photoreceptor cells, among others. Vertebrate sal orthologues (spalt-like/sall) have also important developmental roles during neural development and organogenesis, and at least two human sall genes are linked to the genetic diseases Townes Brocks Syndrome (TBS; SALL1 ) and Okihiro Syndrome (OS; SALL4). In this review, we will summarize the main characteristics of the sall genes and proteins, pointing out to the similarities in their developmental roles during Drosophila and vertebrate development.

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Repression of Dpp targets in theDrosophilawing by Brinker

Cited by 40 publications

References 43 publications

Genetic Interactions Among scribbler, Atrophin and groucho in Drosophila Uncover Links in Transcriptional Repression

Genetic Interactions Among scribbler, Atrophin and groucho in Drosophila Uncover Links in Transcriptional Repression

Inverse regulation of target genes at the brink of the BMP morphogen activity gradient

Regulation and function of Spalt proteins during animal development

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