Reprint of: Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities

Munné, Santiago; Alikani, Mina; Tomkin, Giles; Grifo, J.; Cohen, Jacques

doi:10.1016/j.fertnstert.2019.08.076

Cited by 262 publications

(58 citation statements)

References 27 publications

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“…In the 1990s, from the observation of day 3 embryos fragmentation, it was hypothesised that elimination of selected blastomeres may reflect embryo effort in restoring or maintaining viability when anomalies affect particular blastomeres as, for instance, limited diploid mosaicism, preventing their contribution to the inner cell mass or the trophectoderm of the blastocyst (Alikani et al, 1999;Munné et al, 1995). Nowadays, the technology implementation allows an indepth analysis of these issues.…”

Section: Discussionmentioning

confidence: 99%

Embryos with morphokinetic abnormalities may develop into euploid blastocysts

Lagalla¹,

Tarozzi²,

Sciajno³

et al. 2017

Reproductive BioMedicine Online

124

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Irregular cleavage divisions are expected to produce chromosomally deviant embryos. We investigated whether embryos from irregular cleavages could develop into euploid blastocysts, and, if so, whether any evidence existed of a self-correction mechanism of the embryo. We also investigated the role of different dynamic aspects of morula compaction in this process. A total of 791 embryos from 141 patients undergoing pre-implantation genetic screening were retrospectively analysed using a time-lapse imaging system, and multiple cell divisions were evaluated. A total of 276 embryos developed into blastocysts suitable for biopsy and chromosome screening through array-comparative genomic hybridization. As well as testing trophectoderm biopsy specimens for aneuploidy, excluded cells of 18 blastocysts, which developed from partially compacted morulas, were also analysed. Unique data on the developmental fate of embryos with cleavage abnormalities are presented, and a potential mechanism of 'aneuploidy rescue' is postulated through which mosaic embryos may form partially compacted morulas to exclude aneuploid cells. In addition, this process seems to be less efficient in older women. The data obtained also provide further evidence that excluded cells should not be used to infer the cytogenetic status of the embryo.

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Section: Discussionmentioning

confidence: 99%

Embryos with morphokinetic abnormalities may develop into euploid blastocysts

Lagalla¹,

Tarozzi²,

Sciajno³

et al. 2017

Reproductive BioMedicine Online

124

View full text Add to dashboard Cite

show abstract

“…However, as the age of female patients rises, so does the occurrence of chromosomal abnormalities in the embryos, deriving from defects inherent in the gamete itself or arising at fertilization and/or cleavage. As a result, the miscarriage rate has also increased (Angell, 1994;Eiben et al, 1990;Munné et al, 1995;Nybo Andersen et al, 2000). Cytogenetic analysis of the retained products of conception (POC) is therefore thought to be the most effective test for identifying the cause of miscarriage and recurrent abortions.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic analysis of the retained products of conception after missed abortion following blastocyst transfer: a retrospective, large-scale, single-centre study

Segawa

Kuroda²,

Kato³

et al. 2017

Reproductive BioMedicine Online

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Cytogenetic analysis of the retained products of conception (POC) is the most effective test for identifying miscarriage causes. However, there has been no large-scale study limited to blastocyst transfer. This study retrospectively reports the findings of 1030 cases in which POC analysis was performed after missed abortion following single blastocyst transfer performed at the Shinbashi Yume Clinic. We identified 19.4% as normal karyotypes and 80.6% as aneuploid. These cases broke down into: 62.3% trisomy; 7.8% double trisomy; 0.5% triple or quadruple trisomy; 1.3% monosomy 21; 3.2% monosomy X; 0.1% 47,XXY; 1.0% polyploidy; 1.0% mixed; 1.1% embryonic mosaicism; and 2.4% structural anomalies. In samples with normal karyotypes, 49.5% were female while 50.5% were male. The occurrence of trisomy and double trisomy were both significantly more frequent in the ≥38 years group than in the ≤37 years group (P < 0.01). Trisomy was significantly more frequently associated with fetal heartbeat (P < 0.01); double trisomy, polyploidy and normal karyotype were significantly more frequent with no fetal heartbeat (P < 0.01). There was no significant difference in the frequency of chromosomal abnormalities between the number of miscarriages or blastocyst quality. Thus, POC cytogenetic testing is highly valuable for ascertaining the cause of miscarriage.

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“…17,18 Other reports found that cleavage-arrested embryos at 24 h following thawing were nevertheless still able to show some signs of further cleavage up to 48 h after thawing and could be implanted, but these embryos may have had a higher rate of chromosomal aberrations and more frequently ended in clinical abortion. [19][20][21] In this study, there was only 1 canceled cycle in group A2 (at least 1 optimal embryo, overnight culture), but in group B2 (suboptimal embryos, overnight culture), 54 thawed cycles were canceled because no future cleavage was observed after overnight culture. This suggests that optimal embryos may have stronger development potential.…”

Section: Discussionmentioning

confidence: 74%

Influence of different post-thaw culture time on the clinical outcomes of different quality embryos

Wang

Chen

et al. 2019

Adv Clin Exp Med

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Background. In thawed embryo transfer cycles, the most common method is to transfer the embryos after 2 h of culture. Clinical outcomes of frozen-thawed cleavage embryo transfer cycles regarding the embryos status and the culture time of frozen-thawed cleavage embryos were limited and did not elucidate all unclear issues. Objectives. The objective of this study was to examine the clinical outcomes of frozen-thawed cleavage embryo transfer cycles according to the embryos status and the culture time (2 h or overnight). Material and methods. In this retrospective study (5-year period), 1,654 frozen-thawed embryos were analyzed. Firstly, frozen-thawed cleavage embryos were divided into 2 groups according to their status as follows: with at least 1 optimal embryo and without optimal embryos. Secondly, both of them were divided into 2 groups according to the culture time (2 h or overnight). Age of the female, infertility factors, clinical pregnancy, implantation rate, and live birth rate were compared. Results. There were no statistically significant differences in the pregnancy rate, the implantation rate, live birth rate, the miscarriage rate, and the ectopic pregnancy rate in each group. However, the implantation rate increased after 2 h of incubation (41. 1%) compared to overnight incubation (36.0%) in the group with at least 1 optimal day-3 embryo (p < 0.05). The cancellation rate in the suboptimal day-3 embryos group (9. 1%) was higher than in the group containing at least 1 optimal embryo (0.2%) for the long (overnight) culture (p < 0.05). Conclusions. The implantation rate can be improved in the optimal day-3 embryos transferred after 2 h of culture, but not for suboptimal day-3 embryos. Some unnecessary transfers can be avoided after overnight culture because of no further cleavage of the embryos.

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Reprint of: Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities

Cited by 262 publications

References 27 publications

Embryos with morphokinetic abnormalities may develop into euploid blastocysts

Embryos with morphokinetic abnormalities may develop into euploid blastocysts

Cytogenetic analysis of the retained products of conception after missed abortion following blastocyst transfer: a retrospective, large-scale, single-centre study

Influence of different post-thaw culture time on the clinical outcomes of different quality embryos

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