Reprocible RF values in thin-layer adsorption chromatography

Dallas, M.S.J.

doi:10.1016/s0021-9673(00)99868-6

Cited by 80 publications

(6 citation statements)

References 15 publications

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“…Since it was difficult to reproduce RF values exactly from one run to another (Dallas, 1965), RF values were measured relative to those of authentic pigments derived from Phaeodactylum tricornutum, Dwialiella primolecta or other suitable organisms (see text). Since it was difficult to reproduce RF values exactly from one run to another (Dallas, 1965), RF values were measured relative to those of authentic pigments derived from Phaeodactylum tricornutum, Dwialiella primolecta or other suitable organisms (see text).…”

Section: Methodsmentioning

confidence: 99%

“…Identifications of separated pigments were carried out as follows: (i) Their RF values were compared with those of authentic pigments. Since it was difficult to reproduce RF values exactly from one run to another (Dallas, 1965), RF values were measured relative to those of authentic pigments derived from Phaeodactylum tricornutum, Dwialiella primolecta or other suitable organisms (see text).…”

Section: Methodsmentioning

confidence: 99%

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The pigments of some marine phytoplankton species

Riley

Wilson

1967

J. Mar. Biol. Ass.

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Text- fig. 1) Thin-layer chromatographic procedures using silica gel and glucose have been employed for the examination of the pigments of twenty-three species of marine phytoplankton, drawn from a number of classes. Several new xanthophylls have been found including one similar to fucoxanthin which is the principal pigment in an, as yet, undescribed member of the Haptophyceae. The pigment array of a species of Olisthodiscus sp. (Plymouth no. 239) differs considerably from that of members of the Xanthophyceae and this species is probably incorrectly classified.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The pigments of some marine phytoplankton species

Riley

Wilson

1967

J. Mar. Biol. Ass.

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“…Rod Conditioning and Solvent Focusing. In plate TLC the adsorption of water vapor or solvent vapor has long been known to affect Rf values (31). When analyzing seawater samples where there is a very limited quantitative knowledge of lipid class composition, it is obviously important to obtain reproducible R¡ values.…”

Section: Resultsmentioning

confidence: 99%

Determination of lipid class concentrations in seawater by thin-layer chromatography with flame ionization detection

Delmas¹,

Parrish²,

Ackman³

1984

Anal. Chem.

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“…Thin layer chromatography was carried out on plates 20 x 20 cm covered by a layer of MN Silica Gel G/UV254 (Machery, Nagel & Co.). These plates were stored at constant temperature and humidity before use (Dallas, 1965). Each steroid fraction was first applied (in methanol) as a streak on a 0 5 mm layer and was developed in benzene-methanol (9: 1).…”

Section: Methodsmentioning

confidence: 99%

The isolation of a substance very closely resembling the 18‐monoacetate of D‐aldosterone from the venous blood of activated muscle and from contracting muscle

Lockett¹,

Retallack²

1969

The Journal of Physiology

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SUMMARY1. A renally active substance can be extracted in detectable quantity from 150 g flash-frozen active but not from 150 g flash-frozen flaccid striped muscle. This substance is identical to the 18-monoacetate of Daldosterone (18-MA) in RF value in three solvent systems and in biological activity.2. The renally active substance is secreted into the blood stream by active but not by flaccid muscles.3. Bilateral stimulation of decentralized sciatic nerves causes diuresis, natriuresis, a rise in the clearances of creatinine and PAH without change in heart or respiratory rates, in cats under chloralose anaesthesia. These changes can be matched by i.v. infusion of ADH, 0 3-0 45 m-u./kg. min.4. The urinary changes caused by sciatic stimulation are not prevented by transaction of the spinal cord but are abolished by hypophysectomy.5. Hence, like 18-MA, the muscle substance liberates ADH from the neurohypophysis.

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Reprocible RF values in thin-layer adsorption chromatography

Cited by 80 publications

References 15 publications

The pigments of some marine phytoplankton species

The pigments of some marine phytoplankton species

Determination of lipid class concentrations in seawater by thin-layer chromatography with flame ionization detection

The isolation of a substance very closely resembling the 18‐monoacetate of D‐aldosterone from the venous blood of activated muscle and from contracting muscle

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