Reproducibility in the response of soil bacterial community-level physiological profiles from a land use intensification gradient

Gómez, Emma Laura Alfaro; Garland, Jay L.; Conti, M. E.

doi:10.1016/j.apsoil.2003.10.007

Cited by 98 publications

(65 citation statements)

References 37 publications

(54 reference statements)

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“…However, it is a sensitive, reproducible method (Gomez et al 2004) that shows significant matching with culture-independent methods like PLFA (Buyer and Drinkwater 1997;Grayston et al 2004). Using different methods to determine treatment effects on soil microbial diversity or community structure gives a more complete picture than one method.…”

Section: The Levels Of Mbc (3000 Mg Kgmentioning

confidence: 99%

Soil microbial response to wood ash or lime applied to annual crops and perennial grass in an acid soil of northwestern Alberta

Lupwayi¹,

Arshad²,

Azooz³

et al. 2009

Can. J. Soil. Sci.

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More than 90% of acid soils in western Canada are in Alberta, yet the use of agricultural lime is limited because it is expensive. Wood ash, a by-product of pulp and lumber mills, can be used for liming acid soils. We investigated the effects of amending an acid Luvisol with wood ash or lime on soil microbiological properties at Beaverlodge, Alberta. Both soil amendments were applied at a calcium carbonate rate of 6.72 t ha-1, which was 8.40 t ha-1 for wood ash and 7.47 t ha-1 for lime, in 2002. Soil microbial biomass C (MBC) and the functional diversity and community structures of soil bacteria (indicated by substrate utilization patterns) were measured from 2002 to 2005 under barley (Hordeum vulgare L.), canola (Brassica napus L.), field pea (Pisum sativum L.), and timothy grass (Phleum pratense L.). In the rhizosphere, wood ash increased soil MBC between 2.4-fold in 2002 and 1.3-fold in 2005, and lime increased MBC from 3.2-fold in 2002 to 1.3-fold in 2005. In bulk soil, the increases in MBC ranged from 3.0-fold in 2003 to 1.8-fold in 2005 for wood ash, and from 4.9-fold in 2002 to 2.0-fold in 2005 for lime. Crop effects on MBC were not consistent. Because annual crops were grown in rotation, it is possible that the results obtained in one crop were confounded by effects of the preceding crop. In 2003 and 2004, both amendments increased Shannon index (H’) of bacterial functional diversity in the rhizosphere, and similar results were observed in 2005 in bulk soil. Shifts in the functional structure of bacterial communities due to soil amendment were observed in bulk soil, and shifts due to crop effects were observed in the rhizosphere. In 2003, the average soil pH(CaCl2) increased from 4.91 in control treatments of different crops to 6.60 in lime-amended plots and 6.70 in wood ash-amended plots. In 2004, both wood ash and lime significantly increased soil C mineralization (up to 10 d incubation), but basal respiration (11-24 d incubation) was not affected. The large effect (up to about fivefold) of soil amendments on MBC implies that soil acidity is a major limiting factor for biological processes and the productivity of some Luvisolic soils in Alberta. Wood ash could be used to alleviate these limitations. Key words: C mineralization, microbial diversity, microbial biomass, soil amendment, soil acidity

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Section: The Levels Of Mbc (3000 Mg Kgmentioning

confidence: 99%

Soil microbial response to wood ash or lime applied to annual crops and perennial grass in an acid soil of northwestern Alberta

Lupwayi¹,

Arshad²,

Azooz³

et al. 2009

Can. J. Soil. Sci.

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“…(1) Calculated from absorbance values obtained from a set of 23 carbon sources as described Naiman et al, (2009) using the methodology described by Gómez et al, (2004). (2) 45 kg ha -1 of N applied as urea at sowing.…”

Section: Use Of Soil Microorganisms To Improve Plant Growth and Ecosymentioning

confidence: 99%

Use of Soil Microorganisms to Improve Plant Growth and Ecosystem Sustainability

Salamone¹

2012

The Molecular Basis of Plant Genetic Diversity

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“…Vários estudos têm mostrado que diferentes genótipos de plantas influenciam a comunidade microbiana presente na rizosfera, em razão da diferença na sinalização emitida pelas raízes, como exsudatos (Barea et al, 2005;Marschner et al, 2006). O perfil metabólico deve ser considerado indicador relativo de alterações nas comunidades microbianas dos solos (Gomez et al, 2004), e os seus resultados devem ser interpretados com cautela, pois as diferentes Tabela 2. Riqueza de substratos e índice de diversidade de Shannon, na profundidade de 0 a 10 cm, com base na utilização de fontes de C presentes nas microplacas Biolog EcoPlate pelas comunidades bacterianas, em Latossolo Vermelho-Amarelo sob vegetação nativa (cerrado stricto sensu), cultivado com milho e soja sob plantio direto (PD) e preparo convencional (PC) (1) .…”

Section: Resultsunclassified

“…Apesar de apresentar limitações, as microplacas Biolog têm sido usadas com sucesso para monitorar as mudanças nas comunidades bacterianas no solo e em outros ambientes naturais (Gomez et al, 2004;Ros et al, 2008). Portanto, ao se considerar os resultados obtidos, torna-se clara a influência das práticas agrícolas sobre a microbiota do solo.…”

Section: Resultsunclassified

Estrutura metabólica e genética de comunidades bacterianas em solo de cerrado sob diferentes manejos

Souza

Schlemmer

Alencar

et al. 2012

Pesq. agropec. bras.

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O objetivo deste trabalho foi avaliar a estrutura metabólica e genética de comunidades bacterianas em Latossolo de cerrado sob vegetação nativa ou cultivado em sistema de rotação soja/milho sob preparo convencional e plantio direto. Foram utilizadas microplacas EcoPlate para determinar o perfil e a diversidade metabólica das comunidades bacterianas, e eletroforese em gel com gradiente desnaturante (DGGE) para avaliar a estrutura genética. O teste estatístico de Mantel foi utilizado para avaliar a relação entre a estrutura metabólica e a genética. A comunidade bacteriana sob vegetação nativa apresentou perfil metabólico diferente do encontrado em solos cultivados. No solo cultivado com soja sob preparo convencional, o padrão de utilização das fontes de carbono diferenciou-se dos demais tratamentos. Com base nos resultados de DGGE, a comunidade bacteriana sob vegetação nativa apresentou 35% de similaridade com as de áreas cultivadas. Foram formados grupos distintos de comunidades bacterianas do solo entre as áreas sob preparo convencional e plantio direto. Houve correlação significativa de 62% entre as matrizes geradas pelas microplacas EcoPlate e pela DGGE. Variações no perfil metabólico estão relacionadas às variações na estrutura genética das comunidades bacterianas do solo.

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Reproducibility in the response of soil bacterial community-level physiological profiles from a land use intensification gradient

Cited by 98 publications

References 37 publications

Soil microbial response to wood ash or lime applied to annual crops and perennial grass in an acid soil of northwestern Alberta

Soil microbial response to wood ash or lime applied to annual crops and perennial grass in an acid soil of northwestern Alberta

Use of Soil Microorganisms to Improve Plant Growth and Ecosystem Sustainability

Estrutura metabólica e genética de comunidades bacterianas em solo de cerrado sob diferentes manejos

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