Nagle, EF, Nagai, T, Beethe, AZ, Lovalekar, MT, Zera, JN, Connaboy, C, Abt, JP, Beals, K, Nindl, BC, Robertson, RJ, and Lephart, SM. Reliability and validity of a pool-based maximal oxygen uptake test to examine high-intensity short-duration freestyle swimming performance. J Strength Cond Res 33(5): 1208–1215, 2019—A modality-specific swimming protocol to assess maximal oxygen uptake (Vo
2max
sw
) is essential to accurately prescribe and monitor swimming conditioning programs. Consequently, there is a need for a reliable and valid graded intensity swimming pool test to accurately assess Vo
2max
sw
using indirect calorimetry. The purpose of this study was to assess (a) reliability of an intensity self-regulated swimming pool test of Vo
2max
sw
and (b) validity of a Vo
2max
sw
test using performance swim (PS) time as the criterion. Twenty-nine men (n = 15) and women (n = 14) (age, 23 ± 6.4 years; body mass index, 23.5 ± 3.0 kg·m−2) performed 2 swimming pool Vo
2max
sw
trials (Vo
2max
sw
A and Vo
2max
sw
B), and 2 PS tests (45.7 m [31.20 ± 4.5 seconds] and 182 m [159.2 ± 25.5 seconds]). For test-retest reliability (trials A vs. B), strong correlations (p < 0.05) were found for Vo
2max
sw
(ml·kg−1·min−1) (r = 0.899), O2 pulse (ml O2·beat−1) (r = 0.833), and maximum expired ventilatory volume (L·min−1) (r = 0.785). For performance validity, moderately strong correlations (p < 0.05) were found between Vo
2max
sw
A and 45.7-m (r = −0.543) and 182-m (r = −0.486) swim times. The self-regulated graded intensity swimming pool protocol examined presently is a reliable and valid test of Vo
2max
sw
. Studies should consider the suitability of a Vo
2max
sw
test for military personnel, clinical populations, and injured athletes.