Reproducible Electrodeposition Technique for Immobilizing Glucose Oxidase

Electrospray Deposition as a Method for Mass Fabrication of Mono- and Multicomponent Microarrays of Biological and Biologically Active Substances

“…Applying an attracting potential to certain electrodes and a repelling one to the rest of them will result in a preferred deposition of the charged ES products onto the former electrodes. Although this method looks similar to the known electrophoretic deposition of proteins from solution, use of ESD is advantageous since the lack of a direct contact with protein solutions prevents adsorption of solutes onto the electrodes, thus decreasing the cross-contamination of the deposits.…”

AC-electrophoretic deposition of glucose oxidase

“…It was reported that a large amount of GOx could be deposited in the oxygen evolution region (above 1.0 V) (versus AgCl/Ag) (Johnson et al, 1994). Im et al (1995) and Matsumoto et al (2002) reported that the changes in local pH would be the reason for deposition of large amount of enzyme.…”

“…Depending on the applied potential, two approaches can be distinguished: (i) Deposition of the enzyme on the substrate using low applied currents or voltages Matsumoto et al, 2002;Shin and Kim, 1996a,b;Bharathi and Nogami, 2001). This category also includes co-deposition of the enzyme in the presence of other proteins such as collagen and bovine serum albumin (Wang and Vieth, 1973;Johnson et al, 1994), co-deposition of enzyme with platinum salts to form platinum black surface (Johnson, 1991a;Wang et al, 2000;Ikariyama et al, 1987;Gajovic et al, 2000), codeposition of enzyme with polymeric monomers such as pyrrole or phenol (Fortier et al, 1990;Bartlett and Copper, 1993;Curulli and Palleschi, 1997;Vidal et al, 1999;Cosnier, 2000). (ii) Deposition of the enzyme by electrophoresis using high applied currents or voltages (Johnson, 1991a,b;Sakagami et al, 1981).…”

Controlled electrophoretic deposition of multifunctional nanomodules for bioelectrochemical applications

“…Slowly this technique has been gaining increasing interest in the field of biomedical applications . Electrochemically controlled co-deposition of enzymes with other proteins such as collagen and bovine serum albumin (BSA) has been reported (Johnson et al, 1994). Using this electrophoretic deposition approach, controlled immobilization of GOX on a platinum electrode has been achieved in the presence of a non-ionic detergent, Triton X-100 producing a multilayer films (Matsumoto et al, 2002).…”