Reproducible, High-Throughput Synthesis of Colloidal Nanocrystals for Optimization in Multidimensional Parameter Space

Chan, Emory M.; Xu, Chenxu; Mao, A.W.; Han, Gang; Owen, Jonathan S.; Cohen, Bruce E.; Milliron, Delia J.

doi:10.1021/nl100669s

Cited by 209 publications

(230 citation statements)

References 62 publications

Supporting

Mentioning

223

Contrasting

Unclassified

Order By: Relevance

“…Complete control of reaction parameters and conditions can be achieved with automated robotic systems, such as the WANDA system at the Lawrence Berkeley National Laboratory (Fig. 1A) (19). Batch-to-batch variability can be avoided with continuous-flow systems, and incorporation of "in-line" analytical platforms (e.g., absorbance and fluorescence spectroscopy) that feed information back to reaction controllers allows real-time tuning and optimization of products, which is impossible with batch synthesis (20).…”

Section: Nanoparticle Coresmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

905

686

View full text Add to dashboard Cite

Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Recent examples of success include the ultrasensitive detection of cancer biomarkers in human serum and in vivo sensing of methyl mercury. We identify five key materials challenges, including the development of robust mass-scale nanoparticle synthesis methods, and five broader challenges, including the use of simulations and bioinformatics-driven experimental approaches for predictive modeling of biosensor performance. The resultant generation of nanoparticle biosensors will form the basis of high-performance analytical assays, effective multiplexed intracellular sensors, and sophisticated in vivo probes.Evolution has given rise to organisms of staggering complexity. Our now extensive knowledge of biological systems pales in comparison to the remaining mysteries. Unraveling these requires tools that probe the molecular machinery of life and provide detailed feedback on complex networks of subtle interactions. Such tools are cornerstones of biomedical research and practice, and improvements in these lead directly to a better understanding of fundamental biology, monitoring of health, and diagnosis of disease. Colloidal nanoparticle biosensors are a class of biological probe that will not only yield improved biological sensing but also provide a step change in our ability to probe the biomolecular realm. Nanoparticles can act as high-performance sensors because nanomaterials exhibit unique and useful behaviors not present in their bulk form: for example, bright tunable fluorescence from semiconductor nanoparticles and localized surface plasmon resonance (LSPR) phenomena in metallic nanoparticles. These particles exhibit intense responses to incident light (or other stimuli), and the ability to modulate this response by interaction with target analytes makes them excellent biosensor signal transducers. Outputs can be quantitative or qualitative depending on the functionality of

show abstract

Section: Nanoparticle Coresmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

905

686

View full text Add to dashboard Cite

show abstract

“…Currently, the expression of immature-related surface antigens that are specific for pluripotent cells, such as alkaline phosphatase, TRA-1-60 and SSEA1 for the mouse, or SSEA3 and SSEA4 in human systems (Brambrink et al, 2008;Chan et al, 2010), is often evaluated to characterize a reprogrammed clone. However, the definition of fully reprogrammed cells based only on these early markers has promoted continuous debate due to their expression in clones that do not show reactivation of endogenous pluripotent genes.…”

Section: Defining Pluripotency In the Reprogramming Processesmentioning

confidence: 99%

Reprogramming cell fate to pluripotency: the decision-making signalling pathways

Sanges¹,

Cosma²

2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Pluripotency can be defined as the ability of individual cells to initiate all of the lineages of the mature organism in response to signals from the environment. It has long been assumed that during development, pluripotency is progressively and irreversibly lost through a mechanism that requires strict coordination of the signalling pathways involved in cell proliferation, differentiation and migration. However, recent breakthroughs have highlighted evidence that terminally differentiated cells can be reprogrammed into pluripotent stem cells, prompting a re-evaluation of the reversibility of cell differentiation. Generations of pluripotent cells can arise from somatic cells following ectopic expression of specific transcription factors; however, these factors might well not be the unique essential reprogramming factors. Furthermore, they can be the end-point targets of signalling pathways. Indeed, recent evidence shows that modulation of the Wnt/    -catenin, MAPK/ERK, TGF-     or PI3K/Akt signalling pathways strikingly enhances somatic-cell reprogramming. Nevertheless, we still know relatively little about the underlying mechanisms by which somatic cells de-differentiate to pluripotency. In this review, we provide an overview of the signalling pathways promoting the re-acquisition and maintenance of pluripotency and we discuss the possible mechanisms underlying nuclear reprogramming.

show abstract

“…Importantly, Yan et al pioneered the synthesis of lanthanide fluoride NCs via the thermal decomposition of metal trifluoroacetate precursors (31,32). Preparations of β-NaYF 4 -based UCNPs through decomposition of mixed trifluoroacetates (33,34) or through a two-step ripening process using the premade α-NaYF 4 NCs as precursors (35) have subsequently been reported. Despite these recent progresses, the crystal quality and monodispersity of the as-synthesized UCNPs using existing recipes are still far from ideal.…”

mentioning

confidence: 99%

Morphologically controlled synthesis of colloidal upconversion nanophosphors and their shape-directed self-assembly

Collins

Kang

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

424

379

View full text Add to dashboard Cite

We report a one-pot chemical approach for the synthesis of highly monodisperse colloidal nanophosphors displaying bright upconversion luminescence under 980 nm excitation. This general method optimizes the synthesis with initial heating rates up to 100°C∕ minute generating a rich family of nanoscale building blocks with distinct morphologies (spheres, rods, hexagonal prisms, and plates) and upconversion emission tunable through the choice of rare earth dopants. Furthermore, we employ an interfacial assembly strategy to organize these nanocrystals (NCs) into superlattices over multiple length scales facilitating the NC characterization and enabling systematic studies of shape-directed assembly. The global and local ordering of these superstructures is programmed by the precise engineering of individual NC's size and shape. This dramatically improved nanophosphor synthesis together with insights from shape-directed assembly will advance the investigation of an array of emerging biological and energy-related nanophosphor applications.doped nanocrystals | superlattice | lanthanides | luminescence R ecent advances in synthesis and controlled assembly of monodisperse colloidal nanocrystals (NCs) into superlattice structures have enabled their applications in optics (1), electronics (2), magnetic storage (3), etc. Single-and multicomponent superlattices composed of spherical NCs are increasingly studied and a rich family of structures is now accessible (4, 5), where the electronic and magnetic interactions between the constituents gives rise to new cooperative properties (6, 7). New synthetic approaches are yielding nonspherical NCs with physical properties unobtainable by simply tuning the size of the spheres (8-11), providing an even broader array of nanoscale building blocks. The size and shape dependence of NC's biological activity (12, 13) and toxicity (14) is also of intense interest. However, the challenge of precisely controlling particle shape while maintaining uniformity in size and surface functionality has limited studies of NC environmental health and safety just as it has hindered efforts to organize anisotropic building blocks and to establish methods to capture their unique properties in NC superlattice thin films.Lanthanide-doped nanophosphors are an emerging class of optical materials (15). These NCs often possess "peculiar" optical properties [e.g., quantum cutting (16) and photon upconversion (17)], allowing the management of photons that could benefit a variety of areas including biomedical imaging (18, 19) and therapy (20), photovoltaics (16, 21), solid state lighting (22), and display technologies (23). Colloidal upconversion nanophosphors (UCNPs) are capable of converting long-wavelength near-infrared excitation into short-wavelength visible emission through the long-lived, metastable excited states of the lanthanide dopants (24). In contrast to the Stokes-shifted emissions from semiconductor NCs or organic fluorophores and the multiphoton process employing fluorescent dyes, UCNPs offer several ...

show abstract

Reproducible, High-Throughput Synthesis of Colloidal Nanocrystals for Optimization in Multidimensional Parameter Space

Cited by 209 publications

References 62 publications

Colloidal nanoparticles as advanced biological sensors

Colloidal nanoparticles as advanced biological sensors

Reprogramming cell fate to pluripotency: the decision-making signalling pathways

Morphologically controlled synthesis of colloidal upconversion nanophosphors and their shape-directed self-assembly

Contact Info

Product

Resources

About