Reproducing the Biomechanical Environment of the Chondrocyte for Cartilage Tissue Engineering

Statham, Patrick; Jones, Elena; Jennings, Louise; Fermor, Hazel L.

doi:10.1089/ten.teb.2020.0373

Cited by 28 publications

(17 citation statements)

References 159 publications

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“…Chondrocytes as seed cells for constructing tissue-engineered cartilage conform with the physiological state and are the first choice for experimental research and clinical applications [ 35 ]. The in vitro expansion of autologous chondrocytes for the repair of full-thickness cartilage defects of the knee and ankle joints has been used in clinical practice and has been approved by the United States Food and Drug Administration with satisfactory clinical results [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Tissue-Engineered Cartilage Using Decellularized Scaffolds and Chondrocytes

et al. 2022

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In this paper, we aim to explore the application value of tissue engineering for the construction of artificial cartilage in vitro. Chondrocytes from healthy porcine articular cartilage tissue were seeded on articular cartilage extracellular matrix (ACECM) scaffolds and cultivated. Type II collagen immunofluorescent staining was used to assess secretion from the extracellular matrix. Chondrocytes, which were mainly polygonal and cobblestone-shaped, were inoculated on ACECM-oriented scaffolding for 7 days, and the neo-tissue showed translucent shape and toughness. Using inverted and fluorescence microscopy, we found that chondrocytes on the scaffolds performed well in terms of adhesion and growth, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds had good biocompatibility. The inflammatory cell detection, cellular immune response assay and humoral immune response assay showed porcine ACECM scaffolds were used for xenotransplantation without significant immune inflammatory response. All these findings reveal that ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Fabrication of Tissue-Engineered Cartilage Using Decellularized Scaffolds and Chondrocytes

et al. 2022

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“…[89] Limitations include poor characterization of decellularized scaffold composition unless analyzed using proteomic analysis, [64] and poor mechanical properties. [64] The success of decellularized scaffolds in cartilage tissue engineering may be improved by recellularization of the scaffold prior to implantation [90].…”

Section: Tissue Engineering Approaches Using Natural Scaffoldsmentioning

confidence: 99%

Cellular therapy and tissue engineering for cartilage repair

Zelinka¹,

Roelofs²,

Kandel³

et al. 2022

Osteoarthritis and Cartilage

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“…Within their ECM, chondrocytes are surrounded by a narrow (2–4 μm thick) pericellular matrix (PCM) that is both biochemically and biomechanically (Young’s modulus 23–59 kPa) distinct from the ECM (Young’s modulus ≈500 kPa) and together with the ensconced cells is referred to as a chondron ( Mow and Guo, 2002 ; Guilak et al, 2005 ; Gao et al, 2014 ; Chery et al, 2021 ). The extra- and pericellular matrix zones transmit the depth-dependent dynamic mechanical stimuli, comprising a combination of compression, hydrostatic pressure, shear stress, osmotic stress, and tensile strain, to the mechanosensitive chondrocytes, which in turn adjust cartilage metabolism depending on the magnitude, frequency, strain rate and nature of the applied load ( Natenstedt et al, 2015 ; Gilbert et al, 2021 ; Statham et al, 2021 ). The bidirectional reciprocity in cartilage mechano-signaling enables chondrocytes to sense load application, including altered loading patterns, and in turn adjust matrix composition in response to mechanical cues.…”

Section: The (Patho-)physiological Articular Microenvironment As Blue...mentioning

confidence: 99%

“…Mechanosensory activation (e.g., Piezos, TRPs or integrins, etc.) during the osteoarthritic onset and progression is a critical, to date largely ignored, mechanosignaling aspect of microsystems, which must be closely investigated ( Statham et al, 2021 ). Consequently, to improve existing models, we propose to apply anatomical and (bio)mechanical considerations for the next-generation microsystems.…”

Section: Bioengineering Roadmap—requirements For the Next Generation ...mentioning

confidence: 99%

A Progress Report and Roadmap for Microphysiological Systems and Organ-On-A-Chip Technologies to Be More Predictive Models in Human (Knee) Osteoarthritis

Rothbauer

Reihs

Fischer

et al. 2022

Front. Bioeng. Biotechnol.

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Osteoarthritis (OA), a chronic debilitating joint disease affecting hundreds of million people globally, is associated with significant pain and socioeconomic costs. Current treatment modalities are palliative and unable to stop the progressive degeneration of articular cartilage in OA. Scientific attention has shifted from the historical view of OA as a wear-and-tear cartilage disorder to its recognition as a whole-joint disease, highlighting the contribution of other knee joint tissues in OA pathogenesis. Despite much progress in the field of microfluidic systems/organs-on-a-chip in other research fields, current in vitro models in use do not yet accurately reflect the complexity of the OA pathophenotype. In this review, we provide: 1) a detailed overview of the most significant recent developments in the field of microsystems approaches for OA modeling, and 2) an OA-pathophysiology-based bioengineering roadmap for the requirements of the next generation of more predictive and authentic microscale systems fit for the purpose of not only disease modeling but also of drug screening to potentially allow OA animal model reduction and replacement in the near future.

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Reproducing the Biomechanical Environment of the Chondrocyte for Cartilage Tissue Engineering

Cited by 28 publications

References 159 publications

Fabrication of Tissue-Engineered Cartilage Using Decellularized Scaffolds and Chondrocytes

Fabrication of Tissue-Engineered Cartilage Using Decellularized Scaffolds and Chondrocytes

Cellular therapy and tissue engineering for cartilage repair

A Progress Report and Roadmap for Microphysiological Systems and Organ-On-A-Chip Technologies to Be More Predictive Models in Human (Knee) Osteoarthritis

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