Reproduction Techniques for Hardwood Cutting of &lt;i&gt;Tilia amurensis&lt;/i&gt;

Li, Yang; Wang, Hai Nan; Shen, H. Z.

doi:10.4028/www.scientific.net/amr.183-185.1672

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…The flowers are also used for herbal teas and tinctures, which are particularly popular in Europe and in North American herbal medicine practices. Tilia species have taken hold as a crop of interest for both the honey and timber industries, underscoring a need for the development of efficient methods for large-scale propagation (Yoon et al 2005;Yang et al 2011;Panchev 2019;Yang et al 2020).…”

Section: Introductionmentioning

confidence: 99%

High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim

Kim

Park

et al. 2021

J Plant Biotechnol

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This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5~1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

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Section: Introductionmentioning

confidence: 99%

High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim

Kim

Park

et al. 2021

J Plant Biotechnol

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Ortet Age Effect, Anatomy and Physiology of Adventitious Rooting in Tilia mandshurica Softwood Cuttings

Jin

et al. 2022

Forests

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Tilia mandshurica is recognized as one of the most valuable timber, ornamental and nectariferous tree species, and its cutting propagation is very difficult. To evaluate the influence of ortet age on the rooting of T. mandshurica cuttings and the relationship between the variations of nutrients, enzyme activities, endogenous hormones and the formation of adventitious roots, a series of softwood cuttings of T. mandshurica were collected from 2-, 7-, 15- and 20-year-old healthy ortets, respectively. The rooting percentage, mean root number and total root length were investigated after 60 days of cultivation. Phenotypic and anatomical changes and the variations of organic nutrients, enzyme activities and endogenous hormones were measured during adventitious root formation. The results showed that ortet age effect existed in T. mandshurica softwood cuttings. The adventitious rooting of 2-year-old ortets was superior to that of 7-, 15- and 20-year-old ortets. No root primordium was visible in T. mandshurica softwood cuttings before cultivation, and the root primordia were induced after planting. The adventitious roots derived from vascular cambium and callus in cuttings. Soluble sugar increased during root primordium induction and decreased in adventitious root elongation. Soluble protein decreased during root primordium induction and increased in adventitious root elongation. Polyphenol oxidase (PPO) activity increasing and peroxidase (POD) and 3-indoleacetic acid oxidase (IAAO) activities decreasing were conducive to root primordium induction. High 3-indoleacetic acid (IAA) and gibberellin (GA3) level could promote root primordium induction and low IAA and GA3 level was beneficial to adventitious root elongation. Abscisic acid (ABA) and zeatin riboside (ZR) inhibited root primordium induction and adventitious root elongation. IAA, ABA and ZR coordinated with each other in the process of root primordium induction and adventitious root formation. IAA/ABA increasing could promote root primordium induction, and IAA/ZR increasing was beneficial to root primordium induction and adventitious root elongation. The soluble sugar content, PPO activity, IAA/ABA and IAA/ZR of softwood cuttings from 2-year-old ortets were relatively high, which may be the reason for the better adventitious rooting. The present study provides a reference to build a propagation by cuttings technology of linden trees.

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Reproduction Techniques for Hardwood Cutting of <i>Tilia amurensis</i>

Cited by 2 publications

References 4 publications

High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim

High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim

Ortet Age Effect, Anatomy and Physiology of Adventitious Rooting in Tilia mandshurica Softwood Cuttings

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