Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. I. Rays and Skates

García-Salinas, Pablo; Gallego, Víctor; Asturiano, Juan F.

doi:10.3390/ani11071888

Cited by 7 publications

(6 citation statements)

References 37 publications

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“…Thus, the aim of this study is to offer an anatomical guide intended to be useful during sperm obtention procedures, as well as propose preliminary indications to be considered during artificial insemination. This practical guide complements previous work focused on the anatomy of batoids (rays, skates, and close species) and the use of reproductive techniques on them [ 47 ]. The tools presented in both studies are intended to delve into the development of reproductive techniques, such as sperm cryopreservation [ 29 ] or artificial insemination [ 25 ], some of the important steps toward successful and sustainable breeding programs.…”

Section: Introductionmentioning

confidence: 75%

Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. II. Sharks and Chimaeras

García-Salinas

Gallego

Asturiano

2021

Animals

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The chondrichthyan fishes, which comprise sharks, rays, and chimaeras, are one of the most threatened groups of vertebrates on the planet. Given this situation, an additional strategy for the protection of these species could be the ex situ conservation projects developed in public aquaria and research centers. Nevertheless, to increase sustainability and to develop properly in situ reintroduction strategies, captive breeding techniques, such as sperm extraction and artificial insemination, should be developed. These techniques are commonly used in other threatened species and could be also used in chondrichthyans. However, the different reproductive morphologies found in this group can complicate both processes. Therefore, a comparison of the reproductive anatomy of eight distinct chondrichthyans, with an emphasis on those important differences when performing sperm extraction or artificial insemination, is carried out herein. Sharks and chimaeras belonging to the Scyliorhinidae, Carcharhinidae, Centrophoridae, Etmopteridae, Hexanchidae, and Chimaeridae families were obtained from commercial fisheries, public aquaria, and stranding events. In addition, the process of obtaining viable sperm samples through cannulation, abdominal massage, and oviducal gland extraction is described in detail for both living and dead animals.

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Section: Introductionmentioning

confidence: 75%

Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. II. Sharks and Chimaeras

García-Salinas

Gallego

Asturiano

2021

Animals

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“…poecilura (Henderson et al ., 2013). Although the main function of the gland is still being discussed, it is known that its secretions have a positive effect on the activation of the sperm, thus increasing spermatozoa motility (García‐Salinas et al ., 2021b; Hug et al ., 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Semen collection in elasmobranchs can occur via abdominal massage or by using a cannula or catheter, inserted through the urogenital papilla (García‐Salinas et al ., 2021b; Wyfels et al ., 2021). Both methods require knowledge of the anatomy of the species being managed.…”

Section: Discussionmentioning

confidence: 99%

Andrology of shortnose guitarfish Zapteryx brevirostris (Müller & Henle, 1841) (Chondrichthyes, Trygonorrhinidae)

Camilo

Maganhe

Neto³

et al. 2023

Journal of Fish Biology

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The andrological study of a species involves the macro‐ and microscopic analyses of the internal reproductive organs and the evaluation of seminal parameters and ultrastructural characteristics of the spermatozoa. As in other vertebrates, the male reproductive tract in chondrichthyans consists of testes and reproductive ducts (efferent duct, epididymis, Leydig's gland, ductus deferens and seminal vesicle). In this study the authors used three adult specimens of Zapteryx brevirostris from wild capture kept at the Ubatuba Aquarium, Brazil. Semen was collected by abdominal massage over the location of the seminal vesicle, preceded by ultrasonographic evaluation. The semen collected was diluted 1:200 and subject to quantitative and morphological analyses. Ultrastructural analysis was performed using transmission and scanning electron microscopy. Correlation was observed between successful collection and ultrasonographic image of an engorged seminal vesicle, as well as testicles with easily delimitable margins and higher echogenicity. It was possible to identify free spermatozoa with helical filiform appearance, as well as spermatozeugmata. The average sperm concentration resulted in 5 million packets per millilitre and 140 million spermatozoa per millilitre. The sperm nucleus is described as follows: cone shaped, parachromatin sheath less dense than the chromatin of the nucleus, smooth depression of the nuclear fossa, abaxial axoneme 9 + 2 and accessory axonemal columns in positions 3 and 8 and oval shaped, with flattened inner surface in cross‐section. These results broaden the knowledge of the andrology of this species, contributing to ex situ breeding programmes.

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“…Upon arrival, the samples were frozen at −20°C until analysis. Sperm was collected from sexually active adult males through manual clasper pressure (1 ml) according to García-Salinas et al (2021) . The samples were then immediately transferred to sterile polypropylene microcentrifuge tubes (2 ml) and transported and stored using the same protocol described for UL.…”

Section: Methodsmentioning

confidence: 99%

Unraveling Metabolite Provisioning to Offspring Through Parental Fluids: A Case Study of the Brazilian Guitarfish, Pseudobatos horkelii

Wosnick

Leite²,

Giareta³

et al. 2022

Front. Physiol.

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Elasmobranchs have a very distinct metabolism, and many aspects related to the energetic dynamics of these animals remain poorly investigated. The reproductive period is particularly vulnerable for viviparous species, as part of the energy reserves of the parental biomass is reallocated for gamete production and embryo development. In this context, this study aimed to characterize parental metabolite provisioning to the offspring (both sperm and developing embryos) of the Brazilian Guitarfish, Pseudobatos horkelii, through glucose, β-hydroxybutyrate, triglycerides, and total cholesterol determinations in the uterine liquid (UL) and serum of pregnant females and in the seminal fluid (SF) and serum of males during the copulation period. No significant difference was observed for the analyzed markers between the UL and SF. Except for triglycerides, higher in female serum samples, all other energy markers were present at similar concentrations in the serum of both females and males. When comparing female UL and serum, significant differences were observed for triglycerides and total cholesterol. No differences were observed between SF and serum in males. The results indicate that all markers are being made available to offspring, possibly complementary to the yolk in the case of maternal liquid, and as an additional source for sperm mobilization required during egg fertilization in the case of the paternal fluid. Correlations between the markers in the parental matrices were also noted, compatible with the metabolic pathways activated during energy mobilization in vertebrates. Moreover, distinct marker predominance patterns were also noted for both UL and SF. Energy mobilization characterization directed to offspring through parental fluids aids in unraveling metabolic dynamics during the reproduction stage while also providing support for stress physiology studies to evaluate the indirect effects of parental allostatic overload in both sperm and developing embryos. Finally, energy mobilization assessments of parental fluids may also help elucidate how internal fertilization and viviparity evolved in this very distinct taxonomic group.

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Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. I. Rays and Skates

Cited by 7 publications

References 37 publications

Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. II. Sharks and Chimaeras

Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. II. Sharks and Chimaeras

Andrology of shortnose guitarfish Zapteryx brevirostris (Müller & Henle, 1841) (Chondrichthyes, Trygonorrhinidae)

Unraveling Metabolite Provisioning to Offspring Through Parental Fluids: A Case Study of the Brazilian Guitarfish, Pseudobatos horkelii

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