Reproductive effects of prenatal exposure to nonylphenol on zebrafish (Danio rerio)

Yang, Fangxing; Xu, Ying; Ye, Hui

doi:10.1016/j.cbpc.2005.10.012

Cited by 34 publications

(20 citation statements)

References 52 publications

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“…[24] Thus, the present data suggest that this estuary receives urban, industrial, and agricultural pollution, which are consistent with the emergence of endocrine disruption in fish. [25,26] Moreover, the data is in agreement with the above cited endocrine disruption effects in grey mullet. [10] Besides, our results show the importance of monitoring programs in Portuguese estuaries located near highly urbanized and industrialized areas.…”

Section: Development and Validation Of A Hplc-dad Methods 2743supporting

confidence: 93%

Development and Validation of a HPLC‐DAD Method for Determination of Several Endocrine Disrupting Compounds in Estuarine Water

Ribeiro

Tiritan

Rocha

et al. 2007

Journal of Liquid Chromatography & Related Technologies

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This paper presents the development and validation of an analytical procedure, which allows the simultaneous quantification of nine endocrine disrupters (EDCs) in polluted surface waters. The compounds selected for this study were the natural hormones (17b-estradiol and estrone), the synthetic hormone (17a-ethynylestradiol), the bisphenol A, the alkylphenols (4-octylphenol and 4-nonylphenol), and the phytoestrogens (daidzein, genistein, and biochanin A). Briefly, this method consisted of the preconcentration of water samples (2 L) in 500 mg Oasis HLB cartridges, followed by a cleanup step in 1 g silica cartridges, and analysis of all EDCs by High Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD). The chromatographic separation occurred in a RP-18 analytical column in a gradient mode of CH 3 CN:H 2 O (pH 2) at a flow rate of 1 mL/min. The validation parameters revealed that this method was highly specific for all assayed EDCs (.99%), the linearity of the calibration curves, obtained either by external patronization or by fortified matrix calibration techniques, always showed a correlation higher than 0.99 and their intermediate precision and repeatability, evaluated in terms of intra and inter assays, were optimal for all EDCs (RSD , 3%). The precision and accuracy, estimated as the recovery rates that occurred during the preconcentration and the cleanup processes showed, for all EDCs, excellent results (RSD , 3% and recoveries up to 116%), and preconcentration factors (enrichment) up to 10000. The limits of detection (LOD) and quantification (LOQ) were mathematically estimated from the calibration curves. As expected, these values were different for each of the nine assayed EDCs and were higher when calculated from the addition standard calibration curves. Here, 4-octylphenol was the compound with the lowest LOD and LOQ (3.8 ng/L and 12.0 ng/L) and 17a-ethynylestradiol the one with the highest levels for both cited parameters (18.0 ng/L and 54.5 ng/L). Finally, the performance of the method was checked with water samples from a highly polluted area in the Douro river estuary (Portugal). The field results revealed high levels of estrogens (up to 176 ng/L), bisphenol A (up to 5.1 mg/L), alkylphenols (up to 449 ng/L), and phytoestrogens (up to 74 ng/L), in accordance with the high local pollution load, and suggested that this method can be easily and successfully used in water monitoring surveys of, at least, highly polluted estuarine areas.

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Section: Development and Validation Of A Hplc-dad Methods 2743supporting

confidence: 93%

Development and Validation of a HPLC‐DAD Method for Determination of Several Endocrine Disrupting Compounds in Estuarine Water

Ribeiro

Tiritan

Rocha

et al. 2007

Journal of Liquid Chromatography & Related Technologies

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“…Atrazine (ATZ), one of the most extensively used herbicides, exerts numerous direct and indirect adverse effects including gonad differentiation, sex steroid dynamics, plasma Vtg concentration and aromatase activity in fish species [21e23]. Another estrogen-mimicking compound, nonylphenol (NP), a kind of nonionic surfactant, exerts numerous direct and indirect effects in fish species [24,25]. NP has also been shown to adversely impact sexual differentiation and reproduction in fish [26,27].…”

Section: Introductionmentioning

confidence: 99%

Effect of endocrine disrupting chemicals on the transcription of genes related to the innate immune system in the early developmental stage of zebrafish (Danio rerio)

Jin

Chen

Liu

et al. 2010

Fish & Shellfish Immunology

172

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“…Some APs also induced significantly a p53 gene expression, as demonstrated in copepod, indicating the involvement of p53 in such stress-responses (Hwang et al 2010). Recently, Yang et al (2006) also assumed that p53 gene would be closely related to the impacts of endocrine disrupting chemicals (EDCs) on ecosystem biota and human health. They were also determined as potent inhibitors of Ca 2+ -ATPase disrupting Ca 2+ homeostasis in fish, perhaps contributing to cell and tissue damage during key stages in the development and thereby influencing normal endocrine function later in life (Kirk et al 2003).…”

Section: Toxicity and Risk Factorsmentioning

confidence: 95%

Alkylphenol ethoxylates and alkylphenols – update information on occurrence, fate and toxicity in aquatic environment

Kovářová

Blahová

Divišová

et al. 2013

Polish Journal of Veterinary Sciences

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Alkylphenols and their precursors, alkylphenol etoxylates, are a group of manmade chemicals used mainly as surfactants in domestic and industrial applications worldwide. It has been well established that they have endocrine disruption activity, hepatotoxic, genotoxic and other negative effects on animal and human health. In spite of the effort to reduce their use, they persist in the environment not only in industrial but also in remote regions, and were detected in the variety of natural matrices including air, water, soil as well as food products, and human blood and urine worldwide. This article summarizes their occurrence, fate in natural conditions, and toxicity including mode of action. A subject of our concern was the aquatic environment as the most important reservoir and target of their deleterious impact.

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Reproductive effects of prenatal exposure to nonylphenol on zebrafish (Danio rerio)

Cited by 34 publications

References 52 publications

Development and Validation of a HPLC‐DAD Method for Determination of Several Endocrine Disrupting Compounds in Estuarine Water

Development and Validation of a HPLC‐DAD Method for Determination of Several Endocrine Disrupting Compounds in Estuarine Water

Effect of endocrine disrupting chemicals on the transcription of genes related to the innate immune system in the early developmental stage of zebrafish (Danio rerio)

Alkylphenol ethoxylates and alkylphenols – update information on occurrence, fate and toxicity in aquatic environment

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