Reproductive effects of the endocrine disruptor fenarimol on a Baltic amphipod <i>Monoporeia affinis</i>

Jacobson, Therese; Sundelin, Brita

doi:10.1897/04-610r1.1

Cited by 13 publications

(12 citation statements)

References 43 publications

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“…A trend towards fewer fertilised females per male and number of fertilised females could also be seen. Although not all of them are statistically significant, the results presented here give support to earlier findings that fenarimol impairs reproduction of Monoporeia affinis (Jacobson & Sundelin 2006), with repercussions on the next generation. Most crustacean species have chemoreceptors in terms of aesthetascs on the segments of antennulae.…”

Section: Discussionsupporting

confidence: 88%

“…Mating begins in November; embryogenesis proceeds through the winter, and juveniles are released from the marsupium in February or March. M. affinis is semelparous; males die some weeks after mating, whilst spawned females can be observed a couple of months after juveniles are hatched and released from the marsupium (for details see Sundelin & Eriksson 1998, Jacobson & Sundelin 2006.…”

Section: Test Speciesmentioning

confidence: 99%

“…At the termination of the experiment, the amphipods were immediately analysed live under stereomicroscope (80 × magnification) for sexual maturation stage, presence and numbers of embryos, embryo developmental stage and abnormalities. Sexual maturation was determined after visual examination of external secondary sexual characteristics and gonad development (for a more detailed description, see Jacobson & Sundelin 2006). Dead embryos, dead broods and dead oocytes were classified as 'dead eggs'.…”

Section: Test Speciesmentioning

confidence: 99%

“…All samples were analysed according to Chang & O'Connor (1979). Adaptations of the protocol and quality controls are described in Jacobson & Sundelin (2006).…”

Section: Test Speciesmentioning

confidence: 99%

“…Ecdysteroids are cholesterol derivatives controlling moulting and reproduction in insects and crustaceans (Subramoniam 2000). In particular, fenarimol (at 4 µM) has been shown to impair reproduction and embryogenesis in the cladoceran Daphnia magna (Mu & Leblanc 2002) and (at 2 µM) reduce the fertilisation frequency and the ability of males to fertilise females in M. affinis (Jacobson & Sundelin 2006). Fenarimol has, to date, not been in commercial use in Sweden, and the amphipods used in the present study are not likely to have been pre-exposed to this fungicide.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Combined effects of temperature and a pesticide on the Baltic amphipod Monoporeia affinis

Jacobson¹,

Prevodnik²,

Sundelin³

2008

Aquat. Biol.

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Effects of elevated temperature, in combination with exposure to the fungicide fenarimol, on reproduction in the deposit-feeding Baltic amphipod Monoporeia affinis were investigated. Previously, fenarimol was found to cause endocrine disruption in other crustacean species, via the ecdysteroid system. M. affinis were exposed to elevated temperature and/or fenarimol in soft-bottom microcosms during sexual maturation and mating. Elevated temperature and fenarimol (0.7 mg l -1 ) acted synergistically and increased the number of females with dead eggs, with a more than 4-fold incidence compared to exposure to one of the stressors (24 vs. < 5%). Exposure to both stressors also resulted in a negative intrinsic rate of increase, which might indicate a population decline in the field. Elevated temperature impaired sexual maturation in males and females, lowered the number of fertilised females, reduced fecundity and altered embryogenesis. Exposure to fenarimol resulted in a 40% decrease in ecdysteroid levels in sexually mature males and an increase in heat shock protein 60 expression. Ecdysteroid levels were not affected by temperature in either sex or stage of sexual maturation. Our results suggest that increase in the water temperature due to, e.g., global warming would impair reproduction and possibly increase the sensitivity of M. affinis to toxicants.

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Section: Discussionsupporting

confidence: 88%

Section: Test Speciesmentioning

confidence: 99%

Section: Test Speciesmentioning

confidence: 99%

“…All samples were analysed according to Chang & O'Connor (1979). Adaptations of the protocol and quality controls are described in Jacobson & Sundelin (2006).…”

Section: Test Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Combined effects of temperature and a pesticide on the Baltic amphipod Monoporeia affinis

Jacobson¹,

Prevodnik²,

Sundelin³

2008

Aquat. Biol.

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Ovarian cycle and embryonic development in Gammarus fossarum: Application for reproductive toxicity assessment

Geffard

Xuereb

Chaumot

et al. 2010

Enviro Toxic and Chemistry

156

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Among freshwater invertebrates, Gammarus fossarum is an important test organism and is currently used in ecotoxicology for acute and chronic assays; nevertheless, reproductive toxicity test methods are not yet available for these species. In the present study, the reproductive cycle in Gammarus fossarum was characterized in order to propose a reproductive toxicity test encompassing molting, follicle growth, and embryonic development that will provide a better understanding of the mode of action of chemicals disrupting these hormone-regulated processes. A detailed description of the reproductive cycle in Gammarus fossarum was obtained. As in some amphipods, molt and reproductive cycles of G. fossarum females occur concurrently, lasting 30 d at 12°C. Each molt stage is characterized by a specific marsupial embryonic development stage and the size of developing follicles visible on the ovarian membrane. Based on these results, a 21-d reproductive toxicity test is proposed for this species. This new bioassay was applied to identify the specific impact of different stressors: cadmium, methomyl, nonylphenol, and a starvation diet. Good reproducibility was obtained for different endpoints under control conditions and throughout the experiments. Preliminary robust reference values or benchmarks were proposed for these endpoints. Cadmium was found to specially inhibit secondary vitellogenesis. Nonylphenol had a specific concentration-dependent effect on embryonic development, with an increase in the percent abnormality from a concentration of 0.05 µg/L. A restricted food diet led to a significant delay in the molt cycle, which in turn induced inhibition of secondary vitellogenesis.

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Review of the reproductive biology of amphipods and their endocrine regulation: Identification of mechanistic pathways for reproductive toxicants

Hyne¹

2011

Enviro Toxic and Chemistry

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Abstract-The reproductive biology of amphipods is reviewed to update the knowledge of the male and female reproductive processes of oogenesis and spermatogenesis as well as the endocrine systems of amphipods with the aim of advancing studies of reproductive toxicology. The ovarian and reproduction cycles of female gammaridean amphipods are closely correlated with the molt cycle, which is under direct control by the steroid hormone 20-hydroxyecdysone. The ability of males to copulate and subsequently for females to ovulate is restricted to the early postmolt period of the females. New developments in our understanding of the molt cycle and the endocrine regulatory pathways for reproduction using genomics techniques on other crustacean species are also discussed. The arthropod sterol ponasterone A or xenobiotics such as the fungicide fenarimol have been shown to elicit endocrine disruption in some crustaceans by acting as an agonist for 20-hydroxyecdysone at the ecdysone receptor or by inhibiting the synthesis of 20-hydroxyecdysone, respectively, resulting in disruption of molting and reproduction. Recent studies suggest that cadmium can inhibit secondary vitellogenesis in amphipods. Experimental approaches for examining the metabolic pathways associated with ecdysteroid hormonal signaling or metabolism, exoskeleton maintenance and molting, and the regulation of vitellogenin in amphipods are discussed. This information should aid in the identification of useful biomarkers for reproductive toxicity. Environ. Toxicol. Chem.

show abstract

Reproductive effects of the endocrine disruptor fenarimol on a Baltic amphipod Monoporeia affinis

Cited by 13 publications

References 43 publications

Combined effects of temperature and a pesticide on the Baltic amphipod Monoporeia affinis

Combined effects of temperature and a pesticide on the Baltic amphipod Monoporeia affinis

Ovarian cycle and embryonic development in Gammarus fossarum: Application for reproductive toxicity assessment

Review of the reproductive biology of amphipods and their endocrine regulation: Identification of mechanistic pathways for reproductive toxicants

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