Macedonian Veterinary ReviewThe objectives of the present study were to examine the fatty acid (FA) profiles in serum and in the follicular fluid (FF) and the association between polyunsaturated fatty acid level (PUFA) and follicular growth dynamics following induced luteolysis in dairy cows. A total of 29 dairy cows (CL>25mm, follicle≈15mm) at d0 (start of the experiment) were submitted to ultrasound guided transvaginal follicular aspiration for FF collection from the largest follicle and were injected with 500 μg of cloprostenol. The cows were subdivided into Group A1 (n=11) and Group A2 (n=8) resuming follicular growth either from a secondary follicle less than or larger than 8.5mm, respectively, present at the moment of aspiration and Group A0 (n=10) not resuming follicular growth. Follicular development was monitored daily by ultrasonography until the next dominant follicle reached ≈15mm and was subsequently punctured in Group A1 and A2 (d1). Serum and FF samples for FA determination were taken at d0 from all cows and at d1 in Group A1 and A2. No differences were observed between the FA profile in serum nor in FF between sampling days. Regarding the PUFA levels, the serum linoleic acid (C18:2n6) levels at d0 and d1 were significantly higher than in FF, while alpha linolenic acid (C18:3n3) was lower in the serum than in FF, both at d0 and d1. At d0, a tendency for negative correlation between serum and the FF C18:2n6 with subsequent daily follicular growth rate was observed, while, at d1 there was a strong negative correlation between the serum C18:2n6 and daily growth rate (r=-0.71; p=0.0006). The present study revealed similarities of the FA profiles in the serum and in the FF and association between serum and FF PUFA content with the follicular dynamics after induced luteolysis.Key words: polyunsaturated fatty acids, ultrasound guided transvaginal follicular aspiration, cows, follicular growth
INTRODUCTIONIn the last decades, an intensive genetic selection for higher milk production has inevitably contributed to declining fertility in dairy cows (1). Several studies have shown a strong negative relationship between high milk production and fertility (2, 3) particularly during the period of negative energy balance. The latter occurs in the early postpartum period due to the incapability of the cows to meet their energy needs (4). Implementation of dietary fats in the daily ration in an effort to ameliorate negative energy balance could increase (at least part of) cow's energy demands, leading to enhanced reproductive performance (5, 6). However, it was suggested that specific fatty acids (FA), independent of energy status, might be involved and could affect follicle diameter (7) corpus luteum (CL) function (8), oocyte and embryo quality (9), steroidogenesis (10) and prostaglandin synthesis (11). In this respect, the two main families of polyunsaturated fatty acids (PUFA), omega-3 (n-3, linolenic acid, C18:3n3) and omega-6 (n-6, linoleic acid, C18:2n6) were reported to affect fertility (12), although there...