Reproductive status of Tribolium castaneum (Coleoptera: Tenebrionidae) affects its response to infection by Steinernema feltiae (Rhabditida: Steinernematidae)

Kramarz, Paulina; Małek, Dariusz; Gaweł, Maria; Drobniak, Szymon M.; Homa, Joanna

doi:10.14411/eje.2016.039

Cited by 1 publication

(1 citation statement)

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“…A few studies investigated the infection of EPNs on PO activity of host insects (Brivio et al 2002;Walter et al 2008;Ebrahimi et al 2014aEbrahimi et al , 2014bKramarz et al 2016). PO is the key component of insect humeral immune system.…”

Section: Plasma Phenoloxidase Activitymentioning

confidence: 99%

Effect of entomopathogenic nematode, Steinernema feltiae, on survival and plasma phenoloxidase activity of Helicoverpa armigera (Hb) (Lepidoptera: Noctuidae) in laboratory conditions

Ebrahimi

Shiri

Dunphy

2018

Egypt J Biol Pest Control

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Lethal effect of the entomopathogenic nematode, Steinernema feltiae, against prepupae of Helicoverpa armigera (Hb) (Lepidoptera: Noctuidae) in soil was evaluated. Plasma phenoloxidase activity of H. armigera prepupae against S. feltiae also was evaluated. In brief, 20-25 infective juveniles (IJs) of S. feltiae in 20 μl Ringer's solution were injected into the hemolymph of H. armigera prepupae in different time intervals. In order to evaluate the effect of the Ringer's solution, it was also injected into the larvae in a separate experiment as positive control. A third group of non-injected insects was used as negative control. Hemolymph of the insects was collected in different time intervals (0 to 24 h post injection). Phenoloxidase (PO) activity of H. armigera hemolymph was determined spectrophotometrically, using L-Dopa as substrate. The LC 20 , LC 50 , and LC 80 values were 4.5, 19, and 76 IJs per insect, respectively. PO activity assay demonstrated higher levels of PO unit in nematode-injected insects compared to control groups. Nematode-injected insects showed the highest plasma PO activity 8 h post-injection.

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