Reproductive toxicity by exposure to low concentrations of pesticides in Caenorhabditis elegans

Moya, Aldana; Tejedor, Daniela; Manetti, Mariana; Clavijo, Araceli; Pagano, Eduardo; Munarriz, Eliana; Kronberg, Florencia

doi:10.1016/j.tox.2022.153229

Cited by 11 publications

(5 citation statements)

References 96 publications

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“…However, it is difficult to elucidate the mechanism of reproductive toxicity of fluopimomide directly through RKNs, because RKNs require a host with a long life cycle to complete their onset cycle [33]. C. elegans has become a model to study the mechanism of reproductive toxicity instead of RKNs, owing to its short life cycle and large-scale offspring [19]. Thus, the reproductivetoxicity molecular mechanism of fluopimomide was further studied in C. elegans.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies reported that exposure to pesticides reduced brood size and induced reproductive toxicity in C. elegans [29,35]. Moya et al [19] showed that chlorpyrifos and 2,4-dichlorophenoxyacetic acid decreased the brood size, caused abnormal vitellogenin distribution in C. elegans.…”

Section: Discussionmentioning

confidence: 99%

“…Caenorhabditis elegans is a model in reproductive toxicity studies owing to its transparent body, low cost, and rapid reproduction [18]. In previous studies, C. elegans was used to determine the reproductive toxicity of many compounds [19,20]. Yu et al [21] employed C. elegans to determine the effect of long-term plastic pollution on reproductive toxicity, which found that a single long-term maternal nanoplastics exposure caused multiand trans-generational reproduction decline in the nematodes.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Reproductive Toxicity of Fluopimomide in Meloidogyne incognita and Caenorhabditis elegans

Liu,

Zhang

et al. 2023

Agronomy

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Fluopimomide is a new pesticide that is widely applied in agriculture; however, the effects and molecular mechanisms of fluopimomide in inhibiting nematode reproduction remain unknown. In this study, the effects of fluopimomide on the development and infection of Meloidogyne incognita and the reproductive toxicity in Caenorhabditis elegans were evaluated. Results showed that, in comparison to inoculated control, fluopimomide at 0.33, 0.67, and 1.0 mg/kg soil significantly (p < 0.05) delayed M. incognita development and decreased the reproduction in pot experiments. Fluopimomide notably reduced the galls index with a control effect of 78.6%, 67.9%, and 50.0%, respectively. In addition, a dose–response relationship existed between the brood size and germ cell number of C. elegans and fluopimomide concentrations. Compared with the control group, fluopimomide at 1.0 and 5.0 mg/L notably (p < 0.001) increased the number of cell corpses per gonad in the N2 strain of C. elegans by 8.8- and 14.4-fold, respectively. The number of cell corpses per gonad was similar between the fluopimomide treated worms and the control group in mutants of ced-3, ced-4, and ced-9. Further evidence revealed fluopimomide significantly enhanced the expression of cep-1, egl-1, and clk-2, while no obvious effects were observed in their mutants. Taken together, these results indicated that fluopimomide inflicted DNA damage and induced the core apoptosis pathway caused by germ-cell apoptosis, leading to the reduction of the brood size of C. elegans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Reproductive Toxicity of Fluopimomide in Meloidogyne incognita and Caenorhabditis elegans

Liu,

Zhang

et al. 2023

Agronomy

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“…Because of all these characteristics and its sensitivity to toxicants, C. elegans has established itself as an important model organism for research in toxicology (Leung et al, 2008). For instance, the toxicity assessment of pesticides, toluene also used C. elegans as a model organism (Behl et al, 2016; Meyer & Williams, 2014; Moya et al, 2022; Soares et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the toxicity assessment of pesticides, toluene also used C. elegans as a model organism (Behl et al, 2016;Meyer & Williams, 2014;Moya et al, 2022;Soares et al, 2022).…”

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confidence: 99%

Reprotoxicity induced by acute exposure to aqueous tuber extract of Peruvian Maca (Lepidium meyeniiWalp.) inCaenorhabditis elegans

Pilissão

Rodrigues

Porto

et al. 2023

J of Applied Toxicology

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Maca (Lepidium meyenii Walp.) has been used in folk medicine to treat fertility disturbances, a claim that has been evidenced in some studies. However, the clinical trials validating this use have shown paradoxical findings and then maca safety is not well known. This study investigated the effects and mechanisms by which maca affects the reproductive system using an in vivo model, the nematode Caenorhabditis elegans. Tuber maca powder, obtained from local commerce, was used to prepare the aqueous extract. Worms were acutely exposed to maca extracts (40, 120, 240, and 330 μg/μl), and 48 h after treatments, physiological and biochemical assays were conducted. Maca extract caused a significant decrease in total number of eggs and in the number of eggs per worm. These effects were associated to increased lipid peroxidation, reduced triacylglycerol levels, and also impaired vit‐2 (vitellogenin) expression, besides increase in the number of apoptotic germline cells. We have found quantifiable levels of alkaloids in this maca extract, which presence could be related to this toxicity. Collectively, our data suggest that maca extract exposure causes reproductive toxicity to worms that could be, at least in part, associated to both an increase in apoptosis of germline cells and also to a decrease in vitellogenin expression, needed for egg yolk production and, consequently, successful reproduction.

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Border cell population size and oxidative stress in the root apex of Triticum aestivum seedlings exposed to fungicides

Pyatina,

Shishatskaya,

Dorokhin

et al. 2024

Environ Sci Pollut Res

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Reproductive toxicity by exposure to low concentrations of pesticides in Caenorhabditis elegans

Cited by 11 publications

References 96 publications

Evaluation of the Reproductive Toxicity of Fluopimomide in Meloidogyne incognita and Caenorhabditis elegans

Evaluation of the Reproductive Toxicity of Fluopimomide in Meloidogyne incognita and Caenorhabditis elegans

Reprotoxicity induced by acute exposure to aqueous tuber extract of Peruvian Maca (Lepidium meyeniiWalp.) inCaenorhabditis elegans

Border cell population size and oxidative stress in the root apex of Triticum aestivum seedlings exposed to fungicides

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