Reprogramming of Dermal Fibroblasts into Osteo-Chondrogenic Cells with Elevated Osteogenic Potency by Defined Transcription Factors

Wang, Yinxiang; Wu, Ming‐Hoi; Cheung, Martin; Sham, Mai Har; Akiyama, Haruhiko; Chan, Danny; Cheah, Kathryn S.E.

doi:10.1016/j.stemcr.2017.04.018

Cited by 18 publications

(8 citation statements)

References 44 publications

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“…Recently reported that fibroblast cells successfully reprogrammed into neuronal cells by single and multiple factors, [ 27 , 28 ]. Several studies successfully converted the mammalian (human and animals) fibroblast cells into cardiomyocytes, hepatocytes and pancreatic cells and so on [ 29 – 30 ]. Besides, dermal fibroblasts are used as a neurodegenerative disease marker too [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line

et al. 2018

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The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) cells were verified as fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH cells with a plasmid encoding, the SV40 large T-antigens and the transfected cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The cell proliferation rate of the fibroblast cells, PKW-LWHT was faster than the primary cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal fibroblast cell line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.

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Section: Discussionmentioning

confidence: 99%

Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line

et al. 2018

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“…Reprogrammed cells may also represent a breakthrough in the future obtaining of scalable cell sources capable of undergoing endochondral ossification. Specifically, dermal fibroblasts directly reprogrammed through into the chondrogenic lineage doxycycline-inducible human Sox9 were capable of promoting endochondral ossification in vivo [88, 89].…”

Section: Bone Development Mechanismsmentioning

confidence: 99%

Bone physiology as inspiration for tissue regenerative therapies

2018

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The development, maintenance of healthy bone and regeneration of injured tissue in the human body comprise a set of intricate and finely coordinated processes. However, an analysis of current bone regeneration strategies shows that only a small fraction of well-reported bone biology aspects has been used as inspiration and transposed into the development of therapeutic products. Specific topics that include inter-scale bone structural organization, developmental aspects of bone morphogenesis, bone repair mechanisms, role of specific cells and heterotypic cell contact in the bone niche (including vascularization networks and immune system cells), cell-cell direct and soluble-mediated contact, extracellular matrix composition (with particular focus on the non-soluble fraction of proteins), as well as mechanical aspects of native bone will be the main reviewed topics. In this Review we suggest a systematic parallelization of (i) fundamental well-established biology of bone, (ii) updated and recent advances on the understanding of biological phenomena occurring in native and injured tissue, and (iii) critical discussion of how those individual aspects have been translated into tissue regeneration strategies using biomaterials and other tissue engineering approaches. We aim at presenting a perspective on unexplored aspects of bone physiology and how they could be translated into innovative regeneration-driven concepts.

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“…Transdifferentiation is a process in which adult, mature and fully differentiated cells differentiate into another specific terminal cell type via the induction of lineage-specific transcription factors. Previous studies have revealed that fibroblasts can be converted into several lineages, including neurons (62), cardiomyocytes (63), hepatocytes (64) and osteoblasts (65,66) via the ectopic expression of multiple lineage-specific transcription factors or microRNAs (67). Importantly, this approach has also been applied in vivo using a number of lineage-specific transcription factors (68).…”

Section: Introductionmentioning

confidence: 99%

Comparison between curcumin and all‑trans retinoic acid in the osteogenic differentiation of mouse bone marrow mesenchymal stem cells

et al. 2019

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The use of bone marrow mesenchymal stem cells (BMSCs) has great potential in cell therapy, particularly in the orthopedic field. BMSCs represent a valuable renewable cell source that have been successfully utilized to treat damaged skeletal tissue and bone defects. BMSCs can be induced to differentiate into osteogenic lineages via the addition of inducers to the growth medium. The present study examined the effects of all-trans retinoic acid (ATRA) and curcumin on the osteogenic differentiation of mouse BMSCs. Morphological changes, the expression levels of the bone-associated gene markers bone morphogenetic protein 2, runt-related transcription factor and osterix during differentiation, an in vitro mineralization assay, and changes in osteocalcin expression revealed that curcumin supplementation promoted the osteogenic differentiation of BMSCs. By contrast, the application of ATRA increased osteogenic differentiation during the early stages, but during the later stages, it decreased the mineralization of differentiated cells. In addition, to the best of our knowledge, the present study is the first to examine the effect of curcumin on the osteogenic potency of mouse embryonic fibroblasts (MEFs) after reprogramming with human lim mineralization protein (hLMP-3), which is a positive osteogenic regulator. The results revealed that curcumin-supplemented culture medium increased hLMP-3 osteogenic potency compared with that of MEFs cultured in the non-supplemented medium. The present results demonstrate that enrichment of the osteogenic culture medium with curcumin, a natural osteogenic inducer, increased the osteogenic differentiation capacity of BMSCs as well as that of MEFs reprogrammed with hLMP-3.

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Reprogramming of Dermal Fibroblasts into Osteo-Chondrogenic Cells with Elevated Osteogenic Potency by Defined Transcription Factors

Cited by 18 publications

References 44 publications

Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line

Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line

Bone physiology as inspiration for tissue regenerative therapies

Comparison between curcumin and all‑trans retinoic acid in the osteogenic differentiation of mouse bone marrow mesenchymal stem cells

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