Reprogramming of metabolism in immune-mediated cells

Wada, Jun

doi:10.1007/s13340-017-0321-3

Cited by 7 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All these expensive events are supported by the burst of energy represented, at 3HPLT, released by proteins (red cluster) related to energetic homeostasis (Fig 4). In fact, processes such as phagocytosis, which requires actin polymerization, vacuole maturation and acidification, and antimicrobial proteins production, require a large amounts of energy [65][66][67]. Based on our results we have shown that this is provided by the up-modulation of proteins like glucose-6-phosphate isomerase, pyruvate kinase, malate dehydrogenase and enolase.…”

Section: Discussionmentioning

confidence: 62%

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

et al. 2020

View full text Add to dashboard Cite

BackgroundThe immune system of echinoderm sea urchins is characterised by a high degree of complexity that is not completely understood. The Mediterranean sea urchin Paracentrotus lividus coelomocytes mediate immune responses through phagocytosis, encapsulation of nonself particles, and production of diffusible factors including antimicrobial molecules. Details of these processes, and molecular pathways driving these mechanisms, are still to be fully elucidated.

show abstract

Section: Discussionmentioning

confidence: 62%

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…1B). Indeed, these cells have been known to be dependent on glycolysis for energy production and contain fewer mitochondria [26][27][28][29][30], suggesting that the different expression levels of metabolic enzymes may reflect distinct metabolic state of the cells to some extent.…”

Section: Resultsmentioning

confidence: 99%

Threonine dehydrogenase regulates neutrophil homeostasis but not H3K4me3 levels in zebrafish

Li,

Wang,

Zhang

et al. 2024

The FEBS Journal

View full text Add to dashboard Cite

l‐threonine dehydrogenase (Tdh) is an enzyme that links threonine metabolism to epigenetic modifications and mitochondria biogenesis. In vitro studies show that it is critical for the regulation of trimethylation of histone H3 lysine 4 (H3K4me3) levels and cell fate determination of mouse embryonic stem cells (mESCs). However, whether Tdh regulates a developmental process in vivo and, if it does, whether it also primarily regulates H3K4me3 levels in this process as it does in mESCs, remains elusive. Here, we revealed that, in zebrafish hematopoiesis, tdh is preferentially expressed in neutrophils. Knockout of tdh causes a decrease in neutrophil number and slightly suppresses their acute injury‐induced migration, but, unlike the mESCs, the level of H3K4me3 is not evidently reduced in neutrophils sorted from the kidney marrow of adult tdh‐null zebrafish. These phenotypes are dependent on the enzymatic activity of Tdh. Importantly, a soluble supplement of nutrients that are able to fuel the acetyl‐CoA pool, such as pyruvate, glucose and branched‐chain amino acids, is sufficient to rescue the reduction in neutrophils caused by tdh deletion. In summary, our study presents evidence for the functional requirement of Tdh‐mediated threonine metabolism in a developmental process in vivo. It also provides an animal model for investigating the nutritional regulation of myelopoiesis and immune response, as well as a useful tool for high‐throughput drug/nutrition screening.

show abstract

“…Undoubtedly, dysregulation of immune system contributes to the metabolic complications, while the altered energy metabolism will exacerbate immune abnormalities in return. It has been confirmed that metabolism reprogramming is essential for the differentiation of immune cells ( Wada, 2017 ). Taking glycometabolism as an example, glycolysis is always accelerated in inflammatory cells, because inflammation is a highly energy-consuming event, and glycolysis can generate ATP very fast ( Zhao et al, 2020 ).…”

Section: Discussionmentioning

confidence: 98%

α-Mangostin Alleviated HIF-1α-Mediated Angiogenesis in Rats With Adjuvant-Induced Arthritis by Suppressing Aerobic Glycolysis

Jiang

Cheng

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

A previously validated anti-rheumatic compound α-mangostin (MAN) shows significant metabolism regulatory effects. The current study aimed to clarify whether this property contributed to its inhibition on synovial angiogenesis. Male wistar rats with adjuvant-induced arthritis (AIA) were orally treated by MAN for 32 days. Afterwards, biochemical parameters and cytokines in plasma were determined by corresponding kits, and glycometabolism-related metabolites were further accurately quantified by LC-MS method. Anti-angiogenic effects of MAN were preliminarily assessed by joints based-immunohistochemical examination and matrigel plug assay. Obtained results were then validated by experiments in vitro. AIA-caused increase in circulating transforming growth factor beta, interleukin 6, hypoxia inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in blood and local HIF-1α/VEGF expression in joints was abrogated by MAN treatment, and pannus formation within matrigel plugs implanted in AIA rats was inhibited too. Scratch and transwell assays revealed the inhibitory effects of MAN on human umbilical vein endothelial cells (HUVECs) migration. Furthermore, MAN inhibited tubule formation capability of HUVECs and growth potential of rat arterial ring-derived endothelial cells in vitro. Meanwhile, MAN eased oxidative stress, and altered glucose metabolism in vivo. Glycolysis-related metabolites including glucose 6-phosphate, fructose 6-phosphate, 3-phosphoglyceric acid and phosphoenolpyruvic acid in AIA rats were decreased by MAN, while the impaired pyruvate-synthesizing capability of lactate dehydrogenase (LDH) was recovered. Consistently, MAN restored lipopolysaccharide-elicited changes on levels of glucose and LDH in HUVECs culture system, and exerted similar effects with LDH inhibitor stiripentol on glycometabolism and VEGF production as well as tubule formation capability of HUVECs. These evidences show that MAN treatment inhibited aerobic glycolysis in AIA rats, which consequently eased inflammation-related hypoxia, and hampered pathological neovascularization.

show abstract

Reprogramming of metabolism in immune-mediated cells

Cited by 7 publications

References 12 publications

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

Threonine dehydrogenase regulates neutrophil homeostasis but not H3K4me3 levels in zebrafish

α-Mangostin Alleviated HIF-1α-Mediated Angiogenesis in Rats With Adjuvant-Induced Arthritis by Suppressing Aerobic Glycolysis

Contact Info

Product

Resources

About