Reprogramming of mouse fibroblasts into induced pluripotent stem cells with Nanog

Moon, Jung Hwan; Yun, Wonjin; Kim, Jihyun; Hyeon, Solji; Kang, Phil Jun; Park, Gyuman; Kim, Aeree; Oh, Sejong; Whang, Kwang Youn; Kim, Dong Wook; Yoon, Byung Sun; You, Seungkwon

doi:10.1016/j.bbrc.2012.12.149

Cited by 21 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have already confirmed that the lysine residues of histones H3 and/or H4 linked to Nanog gene turn out to be hypersensible to the regulatory functions of acetylation and deacetylation modifications (Hattori et al, ; Moon et al, ; Staszkiewicz et al, ). It is beyond any doubt that Nanog is thought to be one of the predominant transcriptional factors involved in the mechanisms underlying synergistic inter‐protein communication within the framework of Oct4‐Sox2‐Nanog transcriptional network that has been found to be inevitable to induce and maintain the cellular pluripotency and stemness properties (Moon et al, ; Sumer et al, ; Theunissen et al, ). Hattori et al () have explored that lysine moieties of histones H3 and H4 are extensively acetylated at the Nanog locus in ESCs, giving rise to remarkably elevated quantitative profiles of Nanog gene transcripts.…”

Section: Discussionmentioning

confidence: 99%

Expression of pluripotency‐related genes is highly dependent on trichostatin A‐assisted epigenomic modulation of porcine mesenchymal stem cells analysed for apoptosis and subsequently used for generating cloned embryos

Samiec

Romanek

Lipiński

et al. 2019

Animal Science Journal

View full text Add to dashboard Cite

The present study sought to examine whether trichostatin A (TSA)‐assisted epigenetic transformation of porcine bone marrow (BM)‐derived mesenchymal stem cells (BM‐MSCs) affects the transcriptional activities of pluripotency‐related genes (Oct4, Nanog, c‐Myc, Sox2 and Rex1), multipotent stemness‐related gene (Nestin) and anti‐apoptotic/anti‐senescence‐related gene (Survivin). Epigenetically transformed or non‐transformed BM‐MSCs that had been transcriptionally profiled by qRT‐PCR and had been analysed for different stages of apoptosis progression provided a source of nuclear donor cells for the in vitro production of cloned pig embryos. TSA‐mediated epigenomic modulation has been found to enhance the multipotency extent, stemness and intracellular anti‐ageing properties of porcine BM‐MSCs. This has been confirmed by the relative abundances for Nanog, c‐Myc Rex1, Sox2 and Survivin mRNAs in TSA‐exposed BM‐MSCs that turned out to be significantly higher than those of TSA‐unexposed BM‐MSCs. Additionally, TSA‐assisted epigenomic modulation of BM‐MSCs did not impact the caspase‐8 activity, Bax protein expression and the incidence of TUNEL‐positive cells. In conclusion, the considerably elevated quantitative profiles of Sox2, Rex1, c‐Myc, Nanog and Survivin mRNA transcripts seem to trigger improved reprogrammability of TSA‐treated BM‐MSC nuclei in cloned pig embryos that thereby displayed remarkably increased blastocyst formation rates as compared to those noticed for embryos derived from TSA‐untreated BM‐MSCs.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of pluripotency‐related genes is highly dependent on trichostatin A‐assisted epigenomic modulation of porcine mesenchymal stem cells analysed for apoptosis and subsequently used for generating cloned embryos

Samiec

Romanek

Lipiński

et al. 2019

Animal Science Journal

View full text Add to dashboard Cite

show abstract

“…Epithelial BxPC-3 cells were compared with more morphologically diverse Panc-1 cells, and the comparison identified Panc-1 cells, which had higher Snail1 expression and were more poorly differentiated than BxPC-3 cells, as CSC high with a larger ALDH high population [15]. Stem cells' pluripotent capabilities are maintained in part by the polycomb complex protein BMI-1 (Bmi-1), homeobox protein Nanog, sex-determining region Y-box 2 (Sox2), and octamer-binding transcription factor 4 (Oct4) [157][158][159]. Snail1 silencing resulted in a decrease in ALDH, Sox-2, Oct-4, and invasive properties.…”

Section: Snail1 and Cancer Stem Cellsmentioning

confidence: 99%

Central role of Snail1 in the regulation of EMT and resistance in cancer: a target for therapeutic intervention

Kaufhold

Bonavida

2014

J Exp Clin Cancer Res

131

149

View full text Add to dashboard Cite

Snail1 is the founding member of the Snail superfamily of zinc-finger transcription factors, which also includes Snail2 (Slug) and Snail3 (Smuc). The superfamily is involved in cell differentiation and survival, two processes central in cancer research. Encoded by the SNAI1 gene located on human chromosome 20q13.2, Snail1 is composed of 264 amino acids and usually acts as a transcriptional repressor. Phosphorylation and nuclear localization of Snail1, governed by PI3K and Wnt signaling pathways crosstalk, are critical in Snail1’s regulation. Snail1 has a pivotal role in the regulation of epithelial-mesenchymal transition (EMT), the process by which epithelial cells acquire a migratory, mesenchymal phenotype, as a result of its repression of E-cadherin. Snail1-induced EMT involves the loss of E-cadherin and claudins with concomitant upregulation of vimentin and fibronectin, among other biomarkers. While essential to normal developmental processes such as gastrulation, EMT is associated with metastasis, the cancer stem cell phenotype, and the regulation of chemo and immune resistance in cancer. Snail1 expression is a common sign of poor prognosis in metastatic cancer, and tumors with elevated Snail1 expression are disproportionately difficult to eradicate by current therapeutic treatments. The significance of Snail1 as a prognostic indicator, its involvement in the regulation of EMT and metastasis, and its roles in both drug and immune resistance point out that Snail1 is an attractive target for tumor growth inhibition and a target for sensitization to cytotoxic drugs.

show abstract

“…For example, Moon et al . (2013) reported that Nanog, the core guardian of ESC pluripotency , together with Bmi1 can reprogramme mouse fibroblasts into iPSCs, in the absence of Oct4 . Ectopic expression of E‐cadherin can also compensate for exogenous Oct4 requirement and generates iPSCs in concert with Sox2, Klf4 and c‐Myc .…”

Section: Alternative Reprogramming Factors For Ipscsmentioning

confidence: 99%

“…Initially, researchers presumed that Oct4 presence was essentially indispensable for inducing pluripotency, however, recent reports have contradicted this notion. For example, Moon et al (2013) reported that Nanog, the core guardian of ESC pluripotency (77,78), together with Bmi1 can reprogramme mouse fibroblasts into iPS-Cs, in the absence of Oct4 (79). Ectopic expression of E-cadherin can also compensate for exogenous Oct4 requirement and generates iPSCs in concert with Sox2, Klf4 and c-Myc (80).…”

Section: Alternative Factors For Oct4/sox2/klf4mentioning

confidence: 99%

Inducing pluripotency in vitro: recent advances and highlights in induced pluripotent stem cells generation and pluripotency reprogramming

et al. 2015

View full text Add to dashboard Cite

Induced pluripotent stem cells (iPSCs) are considered patient-specific counterparts of embryonic stem cells as they originate from somatic cells after forced expression of pluripotency reprogramming factors Oct4, Sox2, Klf4 and c-Myc. iPSCs offer unprecedented opportunity for personalized cell therapies in regenerative medicine. In recent years, iPSC technology has undergone substantial improvement to overcome slow and inefficient reprogramming protocols, and to ensure clinical-grade iPSCs and their functional derivatives. Recent developments in iPSC technology include better reprogramming methods employing novel delivery systems such as non-integrating viral and non-viral vectors, and characterization of alternative reprogramming factors. Concurrently, small chemical molecules (inhibitors of specific signalling or epigenetic regulators) have become crucial to iPSC reprogramming; they have the ability to replace putative reprogramming factors and boost reprogramming processes. Moreover, common dietary supplements, such as vitamin C and antioxidants, when introduced into reprogramming media, have been found to improve genomic and epigenomic profiles of iPSCs. In this article, we review the most recent advances in the iPSC field and potent application of iPSCs, in terms of cell therapy and tissue engineering.

show abstract

Reprogramming of mouse fibroblasts into induced pluripotent stem cells with Nanog

Cited by 21 publications

References 10 publications

Expression of pluripotency‐related genes is highly dependent on trichostatin A‐assisted epigenomic modulation of porcine mesenchymal stem cells analysed for apoptosis and subsequently used for generating cloned embryos

Expression of pluripotency‐related genes is highly dependent on trichostatin A‐assisted epigenomic modulation of porcine mesenchymal stem cells analysed for apoptosis and subsequently used for generating cloned embryos

Central role of Snail1 in the regulation of EMT and resistance in cancer: a target for therapeutic intervention

Inducing pluripotency in vitro: recent advances and highlights in induced pluripotent stem cells generation and pluripotency reprogramming

Contact Info

Product

Resources

About