Requirement for CRIF1 in RNA interference and Dicer-2 stability

Lim, Su Jun; Scott, Anthony; Xiong, Xi; Vahidpour, Shabnam; Karijolich, John; Guo, Dongdong; Pei, Shanshan; Yu, Yi; Zhou, Rui; Li, Willis X.

doi:10.4161/rna.34381

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…Tara has been previously implicated in PcG/TrxG function [ 14 , 20 ]. We have formerly identified tara in a genetic screen as a potential new component of the RNAi pathway [ 21 ]. Since transcriptional silencing mediated by PcGs in certain scenarios requires RNAi components like Argonaute and Dicer [ 22 ], we wanted to examine whether Tara could potentially connect PcGs to the RNAi machinery.…”

Section: Resultsmentioning

confidence: 99%

The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression

Dutta

2017

PLoS ONE

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The Polycomb group (PcG) proteins have been implicated in epigenetic transcriptional repression in development, stem cell maintenance and in cancer. The chromodomain protein Polycomb (Pc) is a key member of the PcG. Pc binds to the histone mark, trimethylated histone 3 lysine 27 (H3K27me3), to initiate transcriptional repression. How PcG proteins are recruited to target loci is not fully understood. Here we show that the Drosophila SERTA domain protein Taranis (Tara) is involved in transcriptional regulation of Pc target genes. Embryos lacking Tara exhibit a partial homeotic transformation of cuticular the segments, a phenotype associated with the loss of Pc function. Moreover, Drosophila embryos homozygous for a tara hypomorphic allele also misexpress engrailed, a Pc-regulated gene, and this phenotype is associated with the loss of Pc binding to the cis response element in the engrailed enhancer. In relation to that, Pc recruitment is reduced on the salivary gland polytene chromosomes and specifically at the engrailed locus. These results suggest that Tara might be required for positioning Pc to a subset of its target genes.

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Section: Resultsmentioning

confidence: 99%

The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression

Dutta

2017

PLoS ONE

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“…S2 cells stably expressing miR-34 were generated by transfection with pRmHa-3- miR-34 and the selection marker plasmid pHS-neo using the calcium phosphate method, followed by selection in medium containing 400 μg/mL G418 (Calbiochem). dsRNA treatment was performed as described previously [87–89]. Briefly, ~2 × 10 6 S2 cells were seeded in 6-well plates for 24 h and then transfected with 3 μg of the appropriate dsRNA using the calcium phosphate protocol.…”

Section: Methodsmentioning

confidence: 99%

miR-34 Modulates Innate Immunity and Ecdysone Signaling in Drosophila

et al. 2016

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microRNAs are endogenous small regulatory RNAs that modulate myriad biological processes by repressing target gene expression in a sequence-specific manner. Here we show that the conserved miRNA miR-34 regulates innate immunity and ecdysone signaling in Drosophila. miR-34 over-expression activates antibacterial innate immunity signaling both in cultured cells and in vivo, and flies over-expressing miR-34 display improved survival and pathogen clearance upon Gram-negative bacterial infection; whereas miR-34 knockout animals are defective in antibacterial defense. In particular, miR-34 achieves its immune-stimulatory function, at least in part, by repressing the two novel target genes Dlg1 and Eip75B. In addition, our study reveals a mutual repression between miR-34 expression and ecdysone signaling, and identifies miR-34 as a node in the intricate interplay between ecdysone signaling and innate immunity. Lastly, we identify cis-regulatory genomic elements and trans-acting transcription factors required for optimal ecdysone-mediated repression of miR-34. Taken together, our study enriches the repertoire of immune-modulating miRNAs in animals, and provides new insights into the interplay between steroid hormone signaling and innate immunity.

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“…Much work over the past two decades has solidified the notion that regulatory and non-coding RNAs play a key role in regulating innate immunity. For example, virally derived small interfering RNAs can serve as guides for the RNA interference machinery to target viral RNAs for destruction [ 7 – 11 , 33 , 36 – 38 ]. Also, by targeting mRNAs encoding signaling proteins or inflammatory cytokines, mi cro RNA s ( miRNA s) can profoundly impact the magnitude and duration of the inflammatory response [ 39 – 43 ].…”

Section: Introductionmentioning

confidence: 99%

The circular RNA Edis regulates neurodevelopment and innate immunity

Xiong

Liang²,

Liu³

et al. 2022

PLoS Genet

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Circular RNAs (circRNAs) are widely expressed in eukaryotes. However, only a subset has been functionally characterized. We identify and validate a collection of circRNAs in Drosophila, and show that depletion of the brain-enriched circRNA Edis (circ_Ect4) causes hyperactivation of antibacterial innate immunity both in cultured cells and in vivo. Notably, Edis depleted flies display heightened resistance to bacterial infection and enhanced pathogen clearance. Conversely, ectopic Edis expression blocks innate immunity signaling. In addition, inactivation of Edis in vivo leads to impaired locomotor activity and shortened lifespan. Remarkably, these phenotypes can be recapitulated with neuron-specific depletion of Edis, accompanied by defective neurodevelopment. Furthermore, inactivation of Relish suppresses the innate immunity hyperactivation phenotype in the fly brain. Moreover, we provide evidence that Edis encodes a functional protein that associates with and compromises the processing and activation of the immune transcription factor Relish. Importantly, restoring Edis expression or ectopic expression of Edis-encoded protein suppresses both innate immunity and neurodevelopment phenotypes elicited by Edis depletion. Thus, our study establishes Edis as a key regulator of neurodevelopment and innate immunity.

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Requirement for CRIF1 in RNA interference and Dicer-2 stability

Cited by 8 publications

References 37 publications

The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression

The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression

miR-34 Modulates Innate Immunity and Ecdysone Signaling in Drosophila

The circular RNA Edis regulates neurodevelopment and innate immunity

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