The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression

Dutta, Pranabananda; Li, Willis X.

doi:10.1371/journal.pone.0180026

Cited by 4 publications

(14 citation statements)

References 52 publications

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“…Clones expressing both tara and ban did not have an obvious decrease in En expression ( Fig. 3E′), although a reduction in tara has previously been linked to result in increased En expression (34,35). Thus, tara likely regulates ci expression independently of en.…”

Section: Resultsmentioning

confidence: 84%

The Hippo pathway coactivator Yorkie can reprogram cell fates and create compartment-boundary–like interactions at clone margins

2020

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During development, tissue-specific patterns of gene expression are established by transcription factors and then stably maintained via epigenetic mechanisms. Cancer cells often express genes that are inappropriate for that tissue or developmental stage. Here, we show that high activity levels of Yki, the Hippo pathway coactivator that causes overgrowth in Drosophila imaginal discs, can also disrupt cell fates by altering expression of selector genes like engrailed (en) and Ultrabithorax (Ubx). Posterior clones expressing activated Yki can down-regulate en and express an anterior selector gene, cubitus interruptus (ci). The microRNA bantam and the chromatin regulator Taranis both function downstream of Yki in promoting ci expression. The boundary between Yki-expressing posterior clones and surrounding wild-type cells acquires properties reminiscent of the anteroposterior compartment boundary; Hedgehog signaling pathway activation results in production of Dpp. Thus, at least in principle, heterotypic interactions between Yki-expressing cells and their neighbors could activate boundary-specific signaling mechanisms.

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Section: Resultsmentioning

confidence: 84%

The Hippo pathway coactivator Yorkie can reprogram cell fates and create compartment-boundary–like interactions at clone margins

2020

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“…To understand the role of tara in the testis niche, we knocked down tara in the niche using a UAS‐taraRNA i transgene construct that produces dsRNA against tara . As we have previously shown, this UAS‐tara RNA i transgene, when driven by a Tubulin‐Gal4 , results in a 90% reduction in endogenous tara transcripts without affecting Pc levels 19 . We thus took advantage of the UAS‐tara RNA i transgene to knockdown tara in different cell types with different Gal4 lines.…”

Section: Resultsmentioning

confidence: 99%

“…However, neither Nos‐Gal4 > UAS‐taraRNAi nor Upd‐Gal4 > UAS‐taraRNAi showed any appreciable GSC loss phenotype (Figure 3A,B). We also employed the Flp‐FRT technique to create germline clones homozygous for tara loss‐of‐function allele tara 03881 19 . We observed that, multiple tara null GSCs (marked by loss of GFP; N = 21/32 testes) survived even after 10 days post clone induction, comparable to wild‐type control GSCs (N = 19/34 testes) (Figure 3C; Table S2), in agreement with the tara RNAi experiment carried out with the germline Nos‐Gal4 .…”

Section: Resultsmentioning

confidence: 99%

“…As a member of the PcG complex, 19 Tara might be involved in transcriptional repressing of components or targets of the Notch pathway. Indeed, we have shown that Tara can repress the Notch target gene cut (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

“…GBE‐Su(H)‐lacZ , Esg‐Gal4 , and Esg‐Gal4 , Gal80 ts from Henri Jasper (University of Rochester). UAS‐taraRNAi (Transformant ID: 34362) was from the Vienna Drosophila RNAi Center (VDRC; Vienna, Austria) (RNAi knockdown efficiency was previously verified 19 ). UAS‐taraFlag was generated in the lab using the pUAST‐3xFlag vector (Drosophila Genomics Resource Center), as previously described 19 …”

Section: Methodsmentioning

confidence: 99%

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DrosophilaSERTAD domain protein Taranis is required in somatic cells for maintenance of male germline stem cells

Dutta

Nath

et al. 2020

Developmental Dynamics

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Background Polycomb proteins are essential for maintaining stem cell identity across different stem cell niches. However, how they function to maintain stem cell niches is not fully understood. Results Here we show that the SERTAD protein Taranis (Tara), which is a Polycomb‐trithorax group protein, is expressed in the adult testis niche and plays a role in its maintenance in Drosophila. We found that tara is expressed in early cyst cells, likely including somatic cyst stem cells (CySCs) of Drosophila male testis tip region, which houses both germline and somatic cyst stem cells along with the hub cells, forming the stem cell niche. Consistent with its expression, we found that, while loss of tara in germline cells only had minimal effects, tara knockdown in all cells or only in somatic cells of the niche reduced the number of not only somatic cells, but also germline stem cells (GSCs). We further found that Tara might antagonize Notch signaling in CySCs to maintain the stem cell niche. Conclusions Our studies suggest that Tara might function in somatic CySCs for GSC maintenance in the Drosophila testis.

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TARANIS interacts with VRILLE and PDP1 to modulate the circadian transcriptional feedback mechanism inDrosophila

Akpoghiran

Afonso

Zhang

et al. 2023

Preprint

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The molecular clock that generates daily rhythms of behavior and physiology consists of interlocked transcription-translation feedback loops. InDrosophila, the primary feedback loop involving the CLOCK-CYCLE transcriptional activators and the PERIOD-TIMELESS transcriptional repressors is interlocked with a secondary loop involving VRILLE (VRI) and PAR DOMAIN PROTEIN 1 (PDP1), a repressor and activator ofClocktranscription, respectively. Whereas extensive studies have found numerous transcriptional, translational, and post-translational modulators of the primary loop, relatively little is known about the secondary loop. In this study, we demonstrate that TARANIS (TARA), aDrosophilahomolog of the TRIP-Br/SERTAD family of transcriptional coregulators, functions with VRI and PDP1 to modulate the circadian period and rhythm strength inDrosophila. Overexpression of TARA lengthens the circadian period, whereastaramutants exhibit reduced rhythmicity, a faster molecular clock, and reduced expression of the PDF neuropeptide. We find that TARA can form a physical complex with VRI and PDP1, enhancing their repressor and activator functions, respectively. The conserved SERTA domain of TARA is required to regulate the transcriptional activity of VRI and PDP1, and its deletion leads to reduced locomotor rhythmicity. Consistent with TARA’s role in enhancing VRI and PDP1 activity, overexpressingtarahas a similar effect on the circadian period and rhythm strength as simultaneously overexpressingvriandPdp1. Together, our results suggest that TARA modulates circadian behavior by enhancing the transcriptional activity of VRI and PDP1.Statement of SignificanceInternal molecular clocks generating circadian rhythms of around 24 hours broadly impact behavior and physiology, and circadian dysfunction is associated with various neurological and metabolic diseases. TheDrosophilacircadian clock is a valuable model for understanding the molecular mechanisms underlying daily rhythms. In this study, we identify a conserved gene,taranis, as a novel regulator of theDrosophilamolecular clock. We show that TARANIS modulates circadian behavior by physically interacting with and enhancing the transcriptional activity of clock proteins VRILLE and PDP1. Since mammalian homologs of VRILLE and PDP1 also function in the molecular clock, our results have implications for understanding the mammalian circadian clock.

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The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression

Cited by 4 publications

References 52 publications

The Hippo pathway coactivator Yorkie can reprogram cell fates and create compartment-boundary–like interactions at clone margins

The Hippo pathway coactivator Yorkie can reprogram cell fates and create compartment-boundary–like interactions at clone margins

DrosophilaSERTAD domain protein Taranis is required in somatic cells for maintenance of male germline stem cells

TARANIS interacts with VRILLE and PDP1 to modulate the circadian transcriptional feedback mechanism inDrosophila

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