Rescue of a Cytopathic Paramyxovirus from Peripheral Blood Leukocytes in Subacute Sclerosing Panencephalitis

Robbins, Steven J.; Wrzos, Helena; Kline, Anita L.; Tenser, Richard B.; Rapp, Fred

doi:10.1093/infdis/143.3.396

Cited by 22 publications

(24 citation statements)

References 29 publications

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“…However, HPIV-1 and Sendai virus have been detected using PCR in tissue from the ofactory bulbs of mice (247,248). Animal PIV related to HPIV-2 have been found in patients with MS and subacute sclerosing panencephalitis (118,307). Two closely related paramyxoviruses (Hendra and Nipaha viruses) have been shown to cause severe encephalitis and death in children and adults (53,254,265).…”

Section: Clinical Syndromesmentioning

confidence: 99%

Parainfluenza Viruses

2003

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Human parainfluenza viruses (HPIV) were first discovered in the late 1950s. Over the last decade, considerable knowledge about their molecular structure and function has been accumulated. This has led to significant changes in both the nomenclature and taxonomic relationships of these viruses. HPIV is genetically and antigenically divided into types 1 to 4. Further major subtypes of HPIV-4 (A and B) and subgroups/genotypes of HPIV-1 and HPIV-3 have been described. HPIV-1 to HPIV-3 are major causes of lower respiratory infections in infants, young children, the immunocompromised, the chronically ill, and the elderly. Each subtype can cause somewhat unique clinical diseases in different hosts. HPIV are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. These viruses belong to the Paramyxoviridae family, one of the largest and most rapidly growing groups of viruses causing significant human and veterinary disease. HPIV are closely related to recently discovered megamyxoviruses (Hendra and Nipah viruses) and metapneumovirus

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Section: Clinical Syndromesmentioning

confidence: 99%

Parainfluenza Viruses

2003

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“…Simian virus 5 (SV5) was originally isolated from rhesus monkey kidney cell cultures (Hull et al, 1956) but has subsequently been isolated from tissues of other animals including humans (Hsiung, 1972;Robbins et al, 1981 ;Goswami et al, 1984) and dogs (Rosenberg et al, 1971 ;McCandlish et al, 1978). SV5 causes tracheo-bronchitis in dogs and is often associated with kennel cough (McCandlish et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics

Southern

Young

Heaney

et al. 1991

Journal of General Virology

178

122

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Two canine isolates of simian virus 5 (SV5), termed CPI+ and CPI-, were examined for their ability to react with a bank of monoclonal antibodies (MAbs) that had been previously raised against a human isolate of SV5. CPI-virus was originally isolated from the brain of a gnotobiotic dog infected with CPI+ virus and establishes persistent infections more readily than CPI+ in vitro. Of more than 50 MAbs tested, only one (P-k) reacted with CPI+ but not CPI-, enabling distinction between the two canine isolates. It had been shown previously that MAb P-k reacts with an epitope common to both the P and V proteins. In order to characterize further the epitope binding site of this MAb the P/V genes of CPI+ and CPI-were sequenced.There were four nucleotide differences between CPI+ and CPI-, three of which resulted in predicted amino acid substitutions. Synthetic peptides corresponding to regions encompassing these changes were made and radioimmune competition assays were used to identify the epitope binding site of MAb P-k. Sequence comparison of the P/V gene of CPI+ with the published sequence of a monkey isolate of SV5 (W3) revealed 14 nucleotide differences with five amino acid substitutions. The only amino acid substitution observed between CPI+, CPI-and W3 which altered the predicted secondary structures of the P and V proteins was a leueine to proline change that induced a predicted /I-turn and resulted in the loss of binding of MAb P-k.

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“…The origin of SV5 infections in monkeys is open to question (Hsiung, 1972) and it has been proposed that monkeys acquire infection via contact with humans after being brought into captivity (Tribe, 1966). On a number of occasions the isolation of SV5 from human tissue has been reported (Hsiung, 1972;Robbins et al, 1981), including on five separate occasions from the bone marrows of multiple sclerosis patients (Goswami et al, 1984;Mitchell et al, 1978). Limited seroepidemiological studies have indicated that a reasonably high proportion (20 to 40~) of people have antibodies that react with SV5 (Hsiung, 1972;Goswami et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Monoclonal Antibodies to Simian Virus 5 and Their Use in Revealing Antigenic Differences between Human, Canine and Simian Isolates

Randall

Young

Goswami

et al. 1987

Journal of General Virology

157

137

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SUMMARYHybridomas secreting monoclonal antibodies to simian virus 5 (SV5) were obtained following immunization of mice with purified preparations of a human isolate (LN) of SV5. Immune precipitation studies showed that these monoclonal antibodies had specificities for the haemagglutinin-neuraminidase (HN), fusion (F), nucleo-, matrix and phospho-(P) proteins of SV5. By use of a radioimmune competition assay the monoclonal antibodies to the HN protein were assigned to four groups, members of which recognized different antigenic sites on the protein. All the anti-HN antibodies and the anti-F antibody neutralized virus infectivity. The 54 monoclonal antibodies obtained were used to determine whether there were antigenic differences between five human, two canine and one simian isolate of SV5. Although most of the monoclonal antibodies reacted with all isolates, a few did reveal antigenic differences in the HN, F and P proteins. Furthermore, analysis by SDS-PAGE showed that while the electrophoretic mobilities of most of the virus polypeptides of these isolates were similar some differences could be detected. In particular the P protein showed the most marked mobility differences between the human, canine and simian isolates. Slight differences in the mobility of the F1 glycoprotein could also be visualized.

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Rescue of a Cytopathic Paramyxovirus from Peripheral Blood Leukocytes in Subacute Sclerosing Panencephalitis

Cited by 22 publications

References 29 publications

Parainfluenza Viruses

Parainfluenza Viruses

Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics

Isolation and Characterization of Monoclonal Antibodies to Simian Virus 5 and Their Use in Revealing Antigenic Differences between Human, Canine and Simian Isolates

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