2020
DOI: 10.1590/1984-3143-ar2019-0115
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Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models

Abstract: Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). … Show more

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Cited by 4 publications
(5 citation statements)
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“…There have been reports using SCID mice as recipient mice, which are more severely immunodeficient than KSN/slc mice 5 , 17 . There is also a comparative study using BALB/c nude mice and C57BL/6 SCID mice, where goat embryonic ovaries were transplanted, concluding that angiogenesis in the transplanted ovaries was more evident in SCID mice, but no difference was observed in the number of follicles 18 . Since SCID mice are more difficult to maintain as well as expensive, we selected the nude mice to obtain oocytes.…”
Section: Discussionmentioning
confidence: 99%
“…There have been reports using SCID mice as recipient mice, which are more severely immunodeficient than KSN/slc mice 5 , 17 . There is also a comparative study using BALB/c nude mice and C57BL/6 SCID mice, where goat embryonic ovaries were transplanted, concluding that angiogenesis in the transplanted ovaries was more evident in SCID mice, but no difference was observed in the number of follicles 18 . Since SCID mice are more difficult to maintain as well as expensive, we selected the nude mice to obtain oocytes.…”
Section: Discussionmentioning
confidence: 99%
“…ewe, rabbit). Organs from adult or prepubertal animals and embryonic organs can be used depending on the species Pimentel et al, 2020;Gastal et al, 2017). The gonads should be immediately rinsed in 70% ethanol, and then saline solution (0.9 percent NaCl) supplemented with antibiotics (penicillin/streptomycin).…”
Section: Mammalian Speciesmentioning
confidence: 99%
“…Cellular integrity and quality of frozen-thawed ovarian tissue has been explored using tissue culture and the terminal deoxynucleotidyl transferase nick end labelling (TUNEL) assay (Gastal et al, 2017). Similarly, histological analysis with hematoxylin and eosin staining is used to compare the status of the gonads in different maturation phases Pimentel et al, 2020).…”
Section: In Vitro Test In Mammalian Speciesmentioning
confidence: 99%
“…Subjecting one group of (non-threatened) animals (typically immunodeficient and sterilized mice or their equivalent) to highly invasive surgery, such as inserting testicular cell aggregates or cell cultures beneath the kidney capsule, is ethically dubious if the objective is to protect a different group of animals, even though they may be considered to be at heightened risk of extinction (see [ 129 , 133 , 134 , 135 ] for examples of these techniques). Careful ethical justification is needed when proposing such steps for species conservation, including the valid statistically based reassurance that the offspring are not adversely affected because of the varied technologies required.…”
Section: Stem Cells Conservation and Ethicsmentioning
confidence: 99%