Research Article Atherosclerosis: analysis of the eNOS (T786C) gene polymorphism.

Abstract

ABSTRACT. The coronary arteriosclerotic disease is the most common cardiovascular disease. Atherosclerosis affects large-and medium-sized arteries leading to severe thrombosis or artery stenosis that could evolve to myocardial infarction, ischemic stroke, ischemic injury of kidneys and intestines, and several other life-threatening clinical manifestations. Nitric oxide has been shown to be a promising therapeutic agent against cardiovascular diseases. The eNOS gene assumes several important functions, including regulation of vascular tone and regional blood flow, the suppression of vascular smooth muscle cell proliferation, and modulation of leukocyte-endothelium interactions. The T786C polymorphism is an important point mutation, where thymine is changed to cytosine. T786C significantly reduces the activity of the eNOS promoter gene. Two hundred and ninety-seven peripheral blood samples were collected from patients with the previous diagnosis of atherosclerotic disease based on clinical examination and confirmed by imaging methods. Results were compared using the chisquare test and the G-test. In the present study, the TC genotype was more frequent in both case and control groups with no statistically significant difference. Comparing the relation TC/TT and CC genotypes in the case and control groups, there was no statistically significant difference. No significant difference was found when genotypes were analyzed regarding gender and smoking. Our results suggest a strong tendency of the T allele, in single or double dose, to be associated with atherosclerosis that was not confirmed by the scientific data.

Therapeutic Design of Peptide Modulators of the Interaction Between eNOS and p53 in Atherosclerosis

“…NO produced by the activity of eNOS modulate, beyond other functions, the degree of constriction experienced by a blood vessel, cell cycle progression, senescence or apoptosis, immune system cells activity and platelet aggregation [51][52][53][54][55]. Moreover, availability of NO influences cancer, genomic stability and cardiovascular diseases [31,39,56,57]. Regarding the p53 protein, which is coded by the TP53 gene, is known as the guardian of the genome.…”

“…High cholesterol levels, hypertension, diabetes, smoking and other environmental factors lead to a severe pro-inflammatory and a prothrombotic endothelial state [23][24][25][26][27][28]. In addition, genetic polymorphisms influence endothelial dysfunction because several genes and the proteins they code for, exert crucial functions in regulating vascular endothelial stability [29][30][31]. The eNOS gene regulates the levels of nitric oxide, which is vital for several intracellular biological functions, such as vasodilation, vascular homeostasis, protection of arteries against injuries, cellular growth, signaling pathways and immune response among others [32][33][34][35][36][37].…”

“…The eNOS gene regulates the levels of nitric oxide, which is vital for several intracellular biological functions, such as vasodilation, vascular homeostasis, protection of arteries against injuries, cellular growth, signaling pathways and immune response among others [32][33][34][35][36][37]. The eNOS gene has been investigated as a possible biomarker for non-invasive diagnostic and more efficient treatment of cardiovascular diseases [31,[38][39][40].…”

Research Article The <i>eNOS</i> T786C polymorphism is not related to atherosclerosis and cofactors in a Brazilian population

“…Especially in diabetics, it strengthens in this way, the knowledge that the regulation of eNOS becomes is critical for protection against atherosclerosis (Bonomini et al, 2008). In our previous work, our research group showed that the presence of multiple risk factors increased the deleterious effects of the C allele regarding the eNOS (T786C) gene polymorphism (Barbosa et al, 2017).…”

Pharmacogenetic Implications of eNOS Polymorphisms (Glu298Asp, T786C, 4b/4a) in Cardiovascular Drug Therapy

“…NO plays an important role in the normal physiology of the cardiovascular system, and dysfunctional NO signaling has been associated with development and progression of cardiovascular diseases -myocardial infarction, coronary spasm, intra-stent thrombosis, heart failure (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31).…”