Research into crown rot and wilt diseases of strawberries in Queensland

Paynter, Michelle L.; Gomez, Apollo; Ko, L.; Herrington, M. E.

doi:10.17660/actahortic.2016.1117.26

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…In recent years, strawberry crown rot has become a destructive disease in strawberry production. When the vascular bundle is infected, it becomes brown; strawberries eventually wilt due to the inability to absorb water and nutrients, causing considerable economic damage [2]. The main pathogens of strawberry crown rot reported worldwide are the Colletotrichum gloeosporioides complex species and C. acutatum complex species [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Involvement of CYP51A and CYP51B in Growth, Reproduction, Pathogenicity, and Sensitivity to Fungicides in Colletotrichum siamense

Yang

et al. 2023

Agronomy

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Strawberry crown rot is a serious fungal disease that poses a great threat to strawberry production in the growth cycle. The dominant pathogens of strawberry crown rot pathogens were different in different periods. The main pathogen of strawberry crown rot at the seedling stage is unclear. In this study, 74 Colletotrichum spp. were isolated from 100 strawberry plants at the seedling stage. Based on the morphological observations and phylogenetic analysis of multiple genes (ACT, CAL, CHS, GAPDH, and ITS), all 74 tested isolates were identified as C. gloeosporioides species complex, including 69 isolates of C. siamense and 5 isolates of C. fructicola. Colletotrichum siamense is the main pathogen of strawberry crown rot at the seedling stage in Zhejiang, China. The sterol demethylation inhibitors (DMIs) were used to control strawberry crown rot, and their target was the CYP51 gene. The role of the homologous CYP51 gene in growth, reproduction, pathogenicity, and sensitivity to DMI fungicides in C. siamense has not been determined. Our study found that the pathogenicity of CsCYP51A deletion mutants to strawberry leaves and stems was weakened. The hyphae growth rate of CsCYP51B deletion mutants was significantly slower than that of the wild type, but the sporulation and appressorium production rates increased. CsCYP51B deletion mutants had significantly increased pathogenicity to the stem. Deletion of CsCYP51A led to increased sensitivity to prothioconazole, ipconazole, hexaconazole, triadimefon, prochloraz, tebuconazole, metconazole, propiconazole, and difenoconazole. CsCYP51B deletion mutants were more insensitive. Our results indicate that the effect of the homologous CsCYP51 gene on hyphae growth, pathogenicity, and sensitivity to DMI fungicides differs.

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Section: Introductionmentioning

confidence: 99%

Involvement of CYP51A and CYP51B in Growth, Reproduction, Pathogenicity, and Sensitivity to Fungicides in Colletotrichum siamense

Yang

et al. 2023

Agronomy

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“…Strawberry crown rot is a major disease in most strawberry-growing areas, which can lead to serious economic losses. The crown is damaged, causing the above-ground part to be unable to absorb water and nutrients and eventual wilt [3]. Previous studies have shown that the primary pathogen of crown rot in Zhejiang Province is Colletotrichum siamense, followed by C. fructicola [4].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Characterization of Resistance to Pyraclostrobin in Colletotrichum spp. from Strawberry

Hu,

Zhang,

Xiao

et al. 2023

Agronomy

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Strawberry crown rot poses a significant menace to strawberry production during the seedling stage, and the main pathogen is Colletotrichum spp. Pyraclostrobin is one of the main fungicides that have been registered to control anthracnose caused by Colletotrichum spp. The diversity of pathogens and the risk of fungicide resistance may change from year to year. In order to explore the diversity of pathogens causing crown rot and evaluate the resistance risk of pathogens to pyraclostrobin in different years, crown rot samples were collected in Jiande, Zhejiang Province in 2019 and 2021, and the pathogens were identified. Based on the morphological identification and phylogenetic analysis based on ACT, CAL, CHS, GAPDH, and ITS, all 55 strains were identified as C. gloeosporioides species complexes, including 23 C. siamense isolates and 2 C. fructicola isolates in 2019, and all isolates were identified as C. siamense in 2021. C. siamense was the dominant pathogen of strawberry crown rot in 2019 and 2021. The resistance frequencies of the isolates collected in 2019 and 2021 to pyraclostrobin were 69.57% and 100%, respectively. In general, compared to that in 2019, the resistance frequencies of the pathogen to pyraclostrobin increased in 2021. In terms of fitness, there was no significant difference between resistant strains and sensitive strains in the mycelium growth rate, sporulation and spore germination rate. In addition, the resistant mutants exhibited positive cross-resistance to kresoxim-methyl and azoxystrobin. A sequential analysis of cytochrome b gene showed that C. siamense resistance to pyraclostrobin is linked to the G143A point mutation. Our study indicated that the risk of resistance a fungicide gradually increases with the increase in use years, and in order to reduce the emergence and spread of resistant populations, we should choose fungicides of different mechanisms of action for rotation to reduce the risk of resistance development.

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“…Colletotrichum spp. as the causing agents of the above diseases are able to cause irregular circular spots on leaves ( Karimi et al, 2019 ), invade the shortened crown and expands into an annular ring, making strawberry seedlings unable to absorb water and nutrients and killing seedlings ( Paynter et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Colletotrichum Spp. Diversity Between Leaf Anthracnose and Crown Rot From the Same Strawberry Plant

Hu¹,

Zhang²,

et al. 2022

Front. Microbiol.

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Leaf anthracnose (LA) and anthracnose crown rot (ACR) represent serious fungal diseases that pose significant threats to strawberry production. To characterize the pathogen diversity associated with above diseases, 100 strawberry plants, including varieties of “Hongjia,” “Zhangji,” and “Tianxianzui,” were sampled from Jiande and Zhoushan, the primary plantation regions of Zhejiang province, China. A total of 309 Colletotrichum isolates were isolated from crown (150 isolates) and leaves (159 isolates) of affected samples. Among these, 100 isolates obtained from the plants showing both LA and CR symptoms were selected randomly for further characterization. Based on the morphological observations combined with phylogenetic analysis of multiple genes (ACT, ITS, CAL, GAPDH, and CHS), all the 100 tested isolates were identified as C. gloeosporioides species complex, including 91 isolates of C. siamense, 8 isolates of C. fructicola causing both LA and ACR, and one isolate of C. aenigma causing ACR. The phenotypic characteristics of these isolated species were investigated using the BIOLOG phenotype MicroArray (PM) and a total of 950 different metabolic phenotype were tested, showing the characteristics among these isolates and providing the theoretical basis for pathogenic biochemistry and metabolism. The pathogenicity tests showed that even the same Colletotrichum species isolated from different diseased tissues (leaves or crowns) had significantly different pathogenicity toward strawberry leaves and crown. C. siamense isolated from diseased leaves (CSLA) was more aggressive than C. siamense isolated from rotted crown (CSCR) during the infection on “Zhangji” leaves. Additionally, C. fructicola isolated from affected leaf (CFLA) caused more severe symptoms on the leaves of four strawberry varieties compared to C. fructicola isolated from diseased crown (CFCR). For crown rot, the pathogenicity of CSCR was higher than that of CSLA.

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Research into crown rot and wilt diseases of strawberries in Queensland

Cited by 5 publications

References 6 publications

Involvement of CYP51A and CYP51B in Growth, Reproduction, Pathogenicity, and Sensitivity to Fungicides in Colletotrichum siamense

Involvement of CYP51A and CYP51B in Growth, Reproduction, Pathogenicity, and Sensitivity to Fungicides in Colletotrichum siamense

Diversity and Characterization of Resistance to Pyraclostrobin in Colletotrichum spp. from Strawberry

Colletotrichum Spp. Diversity Between Leaf Anthracnose and Crown Rot From the Same Strawberry Plant

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